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Molecular Breeding

, Volume 34, Issue 3, pp 789–803 | Cite as

QTL characterization of resistance to leaf rust and stripe rust in the spring wheat line Francolin#1

  • Caixia Lan
  • Garry M. Rosewarne
  • Ravi P. Singh
  • Sybil A. Herrera-Foessel
  • Julio Huerta-Espino
  • Bhoja R. Basnet
  • Yelun Zhang
  • Ennian Yang
Article

Abstract

Growing resistant wheat varieties is a key method of controlling two important wheat diseases, leaf rust and stripe rust. We analyzed quantitative trait loci (QTL) to investigate adult plant resistance (APR) to these rusts, using 141 F5 RILs derived from the cross ‘Avocet-YrA/Francolin#1’. Phenotyping of leaf rust resistance was conducted during two seasons at Ciudad Obregon, Mexico, whereas stripe rust was evaluated for two seasons in Toluca, Mexico, and one season in Chengdu, China. The genetic map was constructed with 581 markers, including diversity arrays technology and simple sequence repeat. Significant loci for reducing leaf rust severity were designated QLr.cim-1BL, QLr.cim-3BS.1, QLr.cim-3DC, and QLr.cim-7DS. The six QTL that reduced stripe rust severity were designated QYr.cim-1BL, QYr.cim-2BS, QYr.cim-2DS, QYr.cim-3BS.2, QYr.cim-5AL, and QYr.cim-6AL. All loci were conferred by Francolin#1, with the exception of QYr.cim-2DS, QYr.cim-5AL, and QYr.cim-6AL, which were derived from Avocet-YrA. Closely linked markers indicated that the 1BL locus was the pleiotropic APR gene Lr46/Yr29. QYr.cim-2BS was a seedling resistance gene designated as YrF that conferred intermediate seedling reactions and moderate resistance at the adult plant stage in both Mexican and Chinese environments. Significant additive interactions were detected between the six QTL for stripe rust, but not between the four QTL for leaf rust. Furthermore, we detected two new APR loci for leaf rust in common wheat: QLr.cim-3BS.1 and QLr.cim-7DS.

Keywords

Adult plant resistance (APR) genes Brown rust Triticum aestivum Puccinia triticina Yellow rust 

Notes

Acknowledgments

We thank the Grains Research and Development Corporation (GRDC) of Australia for funding this study. We are also very thankful to Dr. Jing Feng, who help to evaluate seedling tests of the population with Chinese stripe rust races CYR31 and CYR32 in China. Editing by Emma Quilligan is also appreciated.

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Copyright information

© Springer Science+Business Media Dordrecht 2014

Authors and Affiliations

  • Caixia Lan
    • 1
  • Garry M. Rosewarne
    • 1
    • 2
  • Ravi P. Singh
    • 1
  • Sybil A. Herrera-Foessel
    • 1
  • Julio Huerta-Espino
    • 3
  • Bhoja R. Basnet
    • 1
  • Yelun Zhang
    • 4
  • Ennian Yang
    • 2
  1. 1.International Maize and Wheat Improvement Center (CIMMYT)MexicoMexico
  2. 2.Key Laboratory of Biology and Genetic Breeding in Wheat (Southwest), Crop Research InstituteSichuan Academy of Agricultural ScienceChengduPeople’s Republic of China
  3. 3.Campo Experimental Valle de México INIFAPChapingo, Edo. de MéxicoMexico
  4. 4.Institute of Cereal and Oil CropsHebei Academy of Agricultural and Forestry Sciences/Hebei Laboratory of Crop Genetics and BreedingShijiazhuangPeople’s Republic of China

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