Abstract
Durum wheat is the second-most widely grown wheat species, and is primarily used in the production of pasta and couscous. The grain utilization of durum wheat is partly related to its very hard kernel texture because of the lack of the D genome and consequentially the Puroindoline genes. Our previous study reported the transformation of durum wheat with the Puroindoline a (Pina) gene. Here, we characterized the transgenic durum wheat lines expressing the Pina gene, and studied the effects of PINA on grain texture and other kernel characteristics. SDS-PAGE and Western blotting results demonstrated that starch-bound PINA levels of Pina-overexpressing lines were lower than that of Pina-positive control, common wheat cv. Chinese Spring, suggesting a weak association of PINA protein with starch granules in the absence of Pinb. Grain hardness analysis and flour milling tests indicated that the overexpression of PINA resulted in decreased grain hardness and increased flour yield in transgenic durum wheat lines. The agronomic performance of the transgenic and control lines was also examined and it was found that no significant differences in measured traits were observed between Pina-overexpressing and control lines in the 2-year field trials. Since grain hardness strongly affects milling and end-use qualities, the development of medium–hard-textured durum wheat lines is not only of significance for our knowledge of grain hardness and Puroindolines, but also has practical implications for plant breeders and food technologists for the expansion of utilization of durum wheat.
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Acknowledgments
The authors acknowledge funding by the National Science and Technology Major Project of China (2013ZX08002-004; 2013ZX08010-004), the National Natural Science Foundation of China (No. 30871524 and No. 31071403), the National Natural Science Foundation of Hubei, China (No. 2010CBD02403 and No. 2011CDB370), Wuhan Municipal S & T research project (201120922286), and the Fundamental Research Funds for the Central Universities, HUST (2011TS150).
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Yin Li and Xiang Mao contributed equally to this article.
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Li, Y., Mao, X., Wang, Q. et al. Overexpression of Puroindoline a gene in transgenic durum wheat (Triticum turgidum ssp. durum) leads to a medium–hard kernel texture. Mol Breeding 33, 545–554 (2014). https://doi.org/10.1007/s11032-013-9971-4
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DOI: https://doi.org/10.1007/s11032-013-9971-4