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Quantitative trait loci regulating sugar moiety of acylsugars in tomato

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Abstract

Acylsugars are broad-spectrum insect resistance sugar esters produced at very high levels by some accessions of the wild tomato, Solanum pennellii. Transferring acylsugar production from S. pennellii LA716 to cultivated tomato through traditional breeding developed the benchmark acylsugar breeding line CU071026. The base moiety of acylsugars (sucrose vs. glucose) can vary among S. pennellii accessions. Additionally the accession S. pennellii LA716 produces almost exclusively acylglucoses, but the breeding line CU071026 derived from S. pennellii LA716 produces exclusively acylsucroses. This study uses a BC1F1 and a BC1F2 population derived from the cross CU071026 × (CU071026 × S. pennellii LA716) to identify and confirm the action of three quantitative trait loci (QTL) on chromosomes 3, 4, and 11. The QTL on chromosomes 3 and 11 are both required for acylglucose production, while addition of the chromosome 4 QTL affects the level of acylglucose produced in the presence of the QTL on chromosomes 3 and 11. A three-way interaction between these acylglucose QTL was confirmed with a post hoc ANOVA. Identification of these three QTL provides a blueprint for breeding to shift acylsucrose production to acylglucose production in tomato breeding lines. The implications of these QTL and two additional QTL affecting total acylsugar level in the BC1F2 are discussed.

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Acknowledgments

We would also like to thank Jean-Luc Jannink for valuable discussions on epistasis. This study was funded by the Hatch funds (NYC-149440) and the Cornell University Vegetable Breeding Institute.

Conflict of interest

The authors have declared no conflict of interests.

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Authors and Affiliations

  1. Department of Plant Breeding and Genetics, Cornell University, 313 Bradfield Hall, Ithaca, NY, 14853, USA

    Brian M. Leckie

  2. Department of Plant Breeding and Genetics, Cornell University, 301 Bradfield Hall, Ithaca, NY, 14853, USA

    Darlene M. De Jong

  3. Department of Plant Breeding and Genetics, Cornell University, 303 Bradfield Hall, Ithaca, NY, 14853, USA

    Martha A. Mutschler

Authors
  1. Brian M. Leckie
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  2. Darlene M. De Jong
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  3. Martha A. Mutschler
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Correspondence to Martha A. Mutschler.

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Leckie, B.M., De Jong, D.M. & Mutschler, M.A. Quantitative trait loci regulating sugar moiety of acylsugars in tomato. Mol Breeding 31, 957–970 (2013). https://doi.org/10.1007/s11032-013-9849-5

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  • DOI: https://doi.org/10.1007/s11032-013-9849-5

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