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Efficient and fine mapping of RMES1 conferring resistance to sorghum aphid Melanaphis sacchari

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Abstract

Melanaphis sacchari causes serious damage to sorghum (Sorghum bicolor (L.) Moench) growth, development and productivity in many countries. A dominant gene (RMES1) conferring resistance to M. sacchari has been found in the grain sorghum variety Henong 16 (HN16), but fine mapping of the RMES1 locus remains to be reported. In this study, genetic populations segregating for RMES1 were prepared with HN16 and BTx623 as parental lines. The latter had been used for sorghum genome sequencing but was found to be susceptible to M. sacchari in this work. A total of 11 molecular markers were mapped to the short arm of chromosome 6 harboring RMES1. The closest markers flanking the RMES1 locus were Sb6m2650 and Sb6rj2776, which delimited a chromosomal region of about 126 kb containing five predicted genes. The utility of the newly identified DNA markers for tagging RMES1 in molecular breeding of M. sacchari resistance, and further efforts in cloning RMES1, are discussed.

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Acknowledgments

This study was supported by grants from Hebei Province Natural Science Foundation (C2010000758) and the State Key Laboratory of Plant Cell and Chromosome Engineering (PCCE-2008-KF-02). We thank Professor Yinghua Huang (USDA-ARS Plant Science Research Laboratory, USA), Drs Ruiheng Du and Guoqing Liu (Institute of Millet Crops, Hebei Academy of Agricultural and Forestry Sciences, China), Jianghui Cui (Agricultural University of Hebei, China), and Wei Xue (Baoding Vocational and Technical College, China) for constructive suggestions on our work.

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Authors and Affiliations

  1. The State Key Laboratory of Plant Cell and Chromosome Engineering, Institute of Genetics and Developmental Biology, Chinese Academy of Sciences, Beijing, 100101, China

    Faming Wang, Yonghua Han, Yutao Shao, Zhenying Dong, Yang Gao, Kunpu Zhang, Xin Liu & Daowen Wang

  2. The State Key Laboratory of Agro-Biotechnology, China Agricultural University, Beijing, 100193, China

    Songmin Zhao & Dawei Li

  3. Graduate University of Chinese Academy of Sciences, Yuquan Road, Beijing, 100039, China

    Yutao Shao

  4. College of Agronomy, Agricultural University of Hebei, Baoding, 071001, China

    Jinhua Chang

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  1. Faming Wang
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  2. Songmin Zhao
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  3. Yonghua Han
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  4. Yutao Shao
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  7. Kunpu Zhang
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  8. Xin Liu
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  9. Dawei Li
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  10. Jinhua Chang
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  11. Daowen Wang
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Correspondence to Jinhua Chang or Daowen Wang.

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Faming Wang and Songmin Zhao contributed equally to this work.

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Wang, F., Zhao, S., Han, Y. et al. Efficient and fine mapping of RMES1 conferring resistance to sorghum aphid Melanaphis sacchari . Mol Breeding 31, 777–784 (2013). https://doi.org/10.1007/s11032-012-9832-6

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  • DOI: https://doi.org/10.1007/s11032-012-9832-6

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