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Physical localization of a novel blue-grained gene derived from Thinopyrum bessarabicum

Molecular Breeding volume 31, pages 195–204 (2013)Cite this article

Abstract

Blue wheat grain contains different groups of pigments that can be used for making specialty foods or as food colorants. Thinopyrum bessarabicum, a wild relative of wheat, carries a blue-grained gene on chromosome 4J. In this study, we analyzed the mitotic chromosomes of 159 F7 lines derived from the cross between Triticum aestivum cv. Chinese Spring (CS) and a CS–Th. bessarabicum amphiploid by using multi-color fluorescence in situ hybridization, genomic in situ hybridization, and newly developed chromosome 4J-specific DNA markers. Intact chromosome 4J and various 4J chromosomal segments were identified in the 159 lines. The blue-grained gene of Th. bessarabicum was physically localized to the region between the centromere and FL0.52 on chromosome arm 4JL. The chromosomal location of this gene differed from the location of previously reported blue-grained genes. In addition, a strong dosage effect was observed with this gene. These results suggest that the blue-grained gene in Th. bessarabicum represents a novel gene locus for blue aleurone, designated BaThb. The wheat lines and 4J chromosome-specific molecular markers developed in this study will facilitate the introgression and utilization of BaThb for wheat nutritional quality improvement.

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Acknowledgments

The authors sincerely thank Dr. Mujeeb-Kazi from CIMMYT, Mexico for providing seeds of the amphiploid of wheat Chinese Spring and Th. bessarabicum, Dr. Gill from the Plant Pathology Department, Kansas State University, Manhattan, KS, USA, for providing clones of pSc119.2 and pAs1, and Dr. Xiwen Cai from the Department of Plant Sciences, North Dakota State University for critical review. This project was supported by the National Natural Science Foundation of China (No. 31170302) and the National 863 Program of China (2012AA101105).

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Authors and Affiliations

  1. State Key Laboratory of Crop Genetics and Germplasm Enhancement, Nanjing Agricultural University, Nanjing, 210095, China

    Yuefeng Shen, Jian Shen,  Dawadondup, Lifang Zhuang, Yanzhi Wang, Jing Pu, Yigao Feng, Xiue Wang & Zengjun Qi

  2. Institute of Agriculture, Tibet Academy of Agricultural and Animal Science, Lhasa, 850032, China

    Dawadondup

  3. Heartland Plant Innovations Inc., 217 Southwind Place, Manhattan, KS, 66502, USA

    Chenggen Chu

Authors
  1. Yuefeng Shen
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  2. Jian Shen
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  3. Dawadondup
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  4. Lifang Zhuang
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  5. Yanzhi Wang
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  6. Jing Pu
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  7. Yigao Feng
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  9. Xiue Wang
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  10. Zengjun Qi
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Correspondence to Zengjun Qi.

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Yuefeng Shen and Jian Shen equally contributed to this manuscript.

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Shen, Y., Shen, J., Dawadondup et al. Physical localization of a novel blue-grained gene derived from Thinopyrum bessarabicum . Mol Breeding 31, 195–204 (2013). https://doi.org/10.1007/s11032-012-9783-y

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  • DOI: https://doi.org/10.1007/s11032-012-9783-y

Keywords

  • Triticum aestivum
  • Thinopyrum bessarabicum
  • Marker-assisted selection
  • Fluorescence in situ hybridization
  • Chromosome variation
  • Seed color