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Marker-assisted development and characterization of a set of Triticum aestivum lines carrying different introgressions from the T. timopheevii genome

Molecular Breeding volume 31, pages 123–136 (2013)Cite this article

Abstract

A set of common wheat introgression lines carrying one or two introgressions from Triticum timopheevii was produced by means of marker-assisted backcross selection. The starting material consisted of two BC1F20 (T. aestivum*2/T. timopheevii) lines with resistance to leaf rust, stem rust, powdery mildew, spot blotch, and loose smut and containing multiple 1At, 2At, 2G, 3AtL, 3GL, 4GL, 5AtL, 5GL, and 6G T. timopheevii chromosome fragments. The two lines were crossed with, and backcrossed three times to common wheat cultivar Saratovskaya 29. In total, 275 BC2F1 and BC3F2 plants were characterized by microsatellite markers and in situ hybridization. Molecular and cytological analyses revealed 38 plants with a single introgression from chromosomes 2G, 5GL, or 6G of T. timopheevii and 72 plants, each with two introgressions, among them three plants carrying a T. timopheevii translocation involving the D genome (2DS.2GL). It was observed that the lengths of fragments introgressed from the At genome were more than halved in the BC2 generation, while the lengths of 2G and 5GL introgressed fragments were only slightly reduced after the third backcross. The introgression lines were tested for resistance to the native Puccinia triticina population of the Western Siberian region of Russia. Lines with a single introgressed 5GL region carrying the major leaf rust resistance locus, QLr.icg-5B, were completely resistant. The presence of two minor resistance loci, QLr.icg-2A and QLr.icg-1A, suppressed disease development and reduced the number of urediniospores by up to 25 % but did not lead to a hypersensitive response. The introgression lines therefore constitute promising sources of new resistance to Puccinia triticina.

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Acknowledgments

This work was supported by the German Research Foundation (DFG, project RO 1055/9-1), and Federal Targeted Program of the Russian Federation (state contract no. P409).

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Authors and Affiliations

  1. Institute of Cytology and Genetics, Siberian Branch, Russian Academy of Sciences, pr. Lavrentieva 10, 630090, Novosibirsk, Russia

    Ekaterina M. Timonova, Irina N. Leonova & Elena A. Salina

  2. Leibniz Institute of Plant Genetics and Crop Plant Research (IPK), Corrensstr. 3, 06466, Gatersleben, Germany

    Marion S. Röder

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  1. Ekaterina M. Timonova
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  2. Irina N. Leonova
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  3. Marion S. Röder
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  4. Elena A. Salina
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Correspondence to Irina N. Leonova.

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Ekaterina M. Timonova and Irina N. Leonova have contributed equally to this work.

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Timonova, E.M., Leonova, I.N., Röder, M.S. et al. Marker-assisted development and characterization of a set of Triticum aestivum lines carrying different introgressions from the T. timopheevii genome. Mol Breeding 31, 123–136 (2013). https://doi.org/10.1007/s11032-012-9776-x

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Keywords

  • Foreground selection
  • Introgression lines
  • SSR
  • In situ hybridization
  • Leaf rust resistance
  • Major and minor resistance loci