Skip to main content

Camelina mutants resistant to acetolactate synthase inhibitor herbicides

Molecular Breeding volume 30, pages 1053–1063 (2012)Cite this article

Abstract

Camelina (Camelina sativa L.) is a low-input oilseed crop of recent interest for sustainable biofuel production. As a relatively new crop in modern agriculture, considerable agronomic and regulatory problems need to be overcome. A common and troublesome problem is sensitivity to residues of acetolactate synthase (ALS) inhibitor herbicides in soils. To develop resistance to those residues, camelina seed were mutagenized by exposure to 0.3% ethyl methane sulfonate and screened at the M2 generation for increased resistance to imazethapyr and sulfosulfuron. Five lines with resistance were identified and characterized. Four mutants, identified in a screen for imazethapyr resistance (IM1, IM6, IM10, and IM18), appeared phenotypically identical and were controlled by the same co-dominant gene. One mutant identified in a screen for sulfosulfuron resistance was phenotypically different but also appears to be controlled by a single co-dominant gene. Further analysis with the IM1 and SM4 mutants confirmed they had increased resistance to imazethapyr, sulfosulfuron, and flucarbazone, with the resistance in the SM4 mutant being the highest. Compared to the wild type, doses of approximately 200 times more imazethapyr, 30 times more sulfosulfuron, and seven times as much flucarbazone were required to reduce plant growth by 50%. Sequence analysis of ALS genes from the SM4 line identified at least eight different genes or alleles. An allele associated with the highest levels of resistance was created by a single base substitution creating an amino acid shift previously found to cause ALS inhibitor resistance in yeast and tobacco.

This is a preview of subscription content, access via your institution.

Access options

Buy single article

Instant access to the full article PDF.

USD 39.95

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Fig. 1
Fig. 2

References

  • Bernasconi P, Woodworth AR, Rosen BA, Subramanian MV, Siehl DL (1995) A naturally occurring point mutation confers broad range tolerance to herbicides that target acertolactate synthase. J Biol Chem 270:17381–17385

    Article  PubMed  CAS  Google Scholar 

  • Chaleff RS, Mauvals CJ (1984) Acetolactate synthase is the site of action of two sulfonylurea herbicides in higher plants. Science 224:1443–1445

    Article  PubMed  CAS  Google Scholar 

  • Chaleff RS, Ray TB (1984) Herbicide resistant mutants from tobacco culture. Science 223:1148–1151

    Article  PubMed  CAS  Google Scholar 

  • Croughan TP (1998) Inventor; board of supervisors of Louisiana State University, assignee. Herbicide resistant rice. U.S. patent 5,773,704

  • Duggleby RG, Pang SS (2000) Acetohydroxyacid synthase. J Biochem Mol Biol 33:1–36

    CAS  Google Scholar 

  • Duggleby RG, McCourt JA, Guddat LW (2008) Structure and mechanism of inhibition of plant acetohydroxyacid synthase. Plant Physiol Biochem 46:309–324

    Article  PubMed  CAS  Google Scholar 

  • Eliason R, Schoenau JJ, Szmigielski AM, Laverty WM (2004) Phyotoxicity and persistence of flucabazone-sodium in soil. Weed Sci 52:857–862

    Article  CAS  Google Scholar 

  • Gehringer A, Friedt W, Lühs W, Snowdon RJ (2006) Genetic mapping of agronomic traits in false flax (Camelina sativa subsp. sativa). Genome 49:1555–1563

    Article  PubMed  CAS  Google Scholar 

  • Gerwick BC, Subramanian MV, Loney-Gallant VI (1990) Mechanism of action of the 1, 2, 4-triazolo[1, 5-a]pryimidines. J Pestic Sci 29:357–364

    Article  CAS  Google Scholar 

  • Hart SE, Saunders JW, Penner D (1992) Chlorsulfuron resistant sugar beet: cross-resistance and physiological basis of resistance. Weed Sci 40:378–383

    CAS  Google Scholar 

  • Haughn GW, Somerville CR (1986) Sulfonylurea-resistant mutants of Arabidopsis thaliana. Mol Gen Genet 204:430–434

    Article  CAS  Google Scholar 

  • Hutcheon C, Ditt RF, Beilstein M, Comai L, Schroeder J, Goldstein E, Shewmaker CK, Nguyen T, De Rocher J, Kiser J (2010) Polyploid genome of Camelina sativa revealed by isolation of fatty acid synthesis genes. BMC Plant Biol 2010(10):233–248

    Article  Google Scholar 

  • Jung S, Le DT, Yoon S, Yoon MY, Kim YT, Choi J (2004) Amino acid residues conferring herbicide resistance in tobacco acetohydroxyacid synthase. Biochem J 383:53–61

    Article  PubMed  CAS  Google Scholar 

  • Laplante J, Rajcan I, Tardif FJ (2009) Multiple allelic forms of acetohydroxyacid synthase are responsible for herbicide resistance in Setaria viridis. Theor Appl Genet 119:577–585

    Article  PubMed  CAS  Google Scholar 

  • Lee Y, Chang AK, Duggleby RG (1999) Effect of mutagenesis synthase at serine 653 of Arabidopsis thaliana acetohydroxyacid synthase on the sensitivity to imidazolinone and sulfonylurea herbicides. FEBS Lett 452:341–345

    Article  PubMed  CAS  Google Scholar 

  • Neff MM, Turk E, Kalishman M (2002) Web-based primer design for single nucleotide polymorphism analysis. Trends Genet 18:613–615

    Article  PubMed  CAS  Google Scholar 

  • Newhouse KE, Singh BK, Shaner DL, Stidham MA (1991) Mutations in corn (Zea mays L.) conferring resistance to imidazolinones. Theor Appl Genet 83:65–70

    Article  CAS  Google Scholar 

  • Ott KH, Kwagh GW, Stockton GW, Sidorov V, Kakefude G (1996) Rational molecular design and genetic engineering of herbicide resistant crops by structure modeling and site-directed mutagenesis of acetohyroxyacid synthase. J Mol Biol 263:359–368

    Article  PubMed  CAS  Google Scholar 

  • Pang SS, Duggleby RD, Guddat LW (2002) Crystal structure of yeast acetohydroxyacid synthase: a target for herbicidal inhibitors. J Mol Biol 317:249–262

    Article  PubMed  CAS  Google Scholar 

  • Ponziak CJ, Huci PJ (2004) Genetic analysis of imidazolinone resistance in mutation-derived lines of common wheat. Crop Sci 44:23–30

    Article  Google Scholar 

  • Ponziak CJ, Birk IT, O’Donoughue LS, Menard C, Huci PJ, Singh BK (2004) Physiological and molecular characterization of mutation-derived imidazolinone resistance in spring wheat. Crop Sci 44:1434–1443

    Article  Google Scholar 

  • Rajasekaran K, Grula JW, Anderson DM (1996) Selection and charachterization of mutant cotton (Gossypium hirsutum L.) cell lines ressistant to sulfonylurea and imidazolinone herbicides. Plant Sci 199:115–124

    Article  Google Scholar 

  • Sabastian SA, Fader GM, Ulrich DR, Forney DR, Chaleff RS (1989) Semidominant soybean mutation for resistance to sulfonylurea herbicides. Crop Sci 29:1403–1408

    Article  Google Scholar 

  • Seefeldt SS, Jensen JE, Fuerst EP (1995) Log-logistic analysis of herbicide dose-response relationships. Weed Technol 9:218–227

    Google Scholar 

  • Senseman SA (2007) Herbicide handbook. Weed Science Society of America, Lawrence, KS

  • Shaner DL, Anderson PC, Stidham MA (1984) Imidazolinones: potent inhibitors of acetohydroxyacid synthase. Plant Physiol 76:545–546

    Article  PubMed  CAS  Google Scholar 

  • Singh BK, Stidham MA, Shaner DL (1988) Assay of acetohydroxyacid synthase. Anal Biochem 171:173–179

    Article  PubMed  CAS  Google Scholar 

  • Stidham MA (1991) Herbicides that inhibit acetohydroxyacid sythase. Weed Sci 39:428–434

    CAS  Google Scholar 

  • Swanson EB, Harrgesel MJ, Arnoldo MJ, Sippell M, Wong RSV (1989) Microspore mutagenesis and selection: canola plants with field resistance to imadozlinone. Theor Appl Genet 78:525–530

    Article  CAS  Google Scholar 

  • Tan S, Evans RR, Dahmer ML, Singh BKShaner DL (2005) Imidazolionone-tolerant crops: history, current status, and future. Pest Manag Sci 61:246–257

    Article  PubMed  CAS  Google Scholar 

  • Thill DC (1997) Sulfonylurea herbicide resistance in plants. US Patent RE35661

  • Tranel PJ, Wright TR (2002) Resistance of weeds to ALS-inhibiting herbicides: what have we learned? Weed Sci 50:100–712

    Article  Google Scholar 

  • Tranel PJ, Wright TR, Heap IM (2011) ALS mutations from herbicide-resistant weeds. http://www.weedscience.org/mutations/mutdisplay.aspx. Last accessed 9 Dec 2011

  • Vollmanna J, Moritza T, Kargla C, Baumgartnerb S, Wagentristl H (2007) Agronomic evaluation of camelina genotypes selected for seed quality characteristics. Indust Crops Prod 26:270–277

    Article  Google Scholar 

  • Westerfeld WW (1945) A colorimetric determination of blood acetoin. J Biol Chem 161:495–502

    CAS  Google Scholar 

  • Whaley CM, Wilson HP, Westwood JH (2007) A new mutation in plant ALs confers resistance to five classes of ALS-inhibiting herebicides. Weed Sci 55:83–90

    Article  CAS  Google Scholar 

  • Wright TR, Penner D (1998) Cell celection and inheritance of imidazolinone resistance in sugar beet (Beta vulgaris). Theor Appl Genet 96:612–620

    Article  CAS  Google Scholar 

  • Yu Q, Zhang XQ, Hashem A, Walsh MJ, Powles SB (2003) ALS gener prolene (197) mutations confer ALS herbicide resistance in eight seperate wild radish (Raphanus raphanistrum). Weed Sci 51:831–838

    Article  CAS  Google Scholar 

  • Zubr J (1997) Oil-seed crop: camelina sativa. Indust Crops Prod 6:113–119

    Article  Google Scholar 

Download references

Acknowledgments

This work was supported by funding from the Sun Grant Initiative and the Washington Biofuels Cropping Systems Research and Extension Project. The authors are grateful to Dr. Chuntao Yin for assistance in characterizing the ALS gene family in camelina. PPNS No. 0586, College of Agricultural, Human, and Natural Resource Sciences, Agricultural Research Center, Project No. WNP00663, Washington State University.

Author information

Authors and Affiliations

  1. Department of Crop and Soil Sciences, Washington State University, Pullman, WA, 99163, USA

    Dustin T. Walsh, Ian C. Burke & Scot H. Hulbert

  2. Department of Plant Pathology, Washington State University, Pullman, WA, 99163, USA

    Ebrahiem M. Babiker & Scot H. Hulbert

Authors
  1. Dustin T. Walsh
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Ebrahiem M. Babiker
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Ian C. Burke
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. Scot H. Hulbert
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to Scot H. Hulbert.

Rights and permissions

Reprints and Permissions

About this article

Cite this article

Walsh, D.T., Babiker, E.M., Burke, I.C. et al. Camelina mutants resistant to acetolactate synthase inhibitor herbicides. Mol Breeding 30, 1053–1063 (2012). https://doi.org/10.1007/s11032-011-9689-0

Download citation

  • Received:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s11032-011-9689-0

Keywords

  • Dose response
  • Seed mutagenesis
  • Herbicide resistance