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Genetic mapping of a fusarium wilt resistance gene in Brassica oleracea

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Abstract

Fusarium wilt caused by Fusarium oxysporum f. sp. conglutinans is one of the most important diseases of Brassica crops, resulting in severe reductions in yield and quality. To characterize the inheritance pattern of fusarium resistance, a cross between a susceptible broccoli and a resistant cabbage was subjected to segregation analysis. Results indicated that resistance was controlled by a single dominant allele. This gene was named Foc-Bo1 and mapped to linkage group seven (O7) by both the segregation test and quantitative trait locus (QTL) analysis. The QTL on O7 was detected with a logarithm of odds score (LOD) of 19.5, which was above the threshold value with genome-wide 1% significance level (2.01). A minor QTL was also detected on O4 with a LOD score of 2.06. Inoculation tests indicated that stable expression of fusarium resistance at high temperatures required Foc-Bo1 homozygosity. The association between Foc-Bo1 and the closest simple sequence repeat marker (KBrS003O1N10) was analyzed in three F3 populations. Based on these studies, KBrS003O1N10 represents an effective marker-assisted selection (MAS) tool for breeding fusarium wilt resistance into Brassica oleracea crops. To our knowledge, this is the first paper to map the fusarium-resistance gene in Brassica species and to validate the effectiveness of MAS in improving fusarium resistance in these important plants.

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Acknowledgments

The authors sincerely thank Dr. Kadota at the National Agricultural Research Center for Tohoku Region, Japan, for kindly providing the F. oxysporum f. sp. conglutinans strain. This work was supported in part by a grant from Japan Science and Technology Agency, Japan (Adaptable and Seamless Technology Transfer Program through Target-Driven R&D No. AS221Z02511).

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Authors and Affiliations

  1. Graduate School of Science and Technology, Niigata University, 2-8050, Ikarashi, Niigata, 950-2181, Japan

    Zi-jing Pu, Motoki Shimizu, Yan-ju Zhang, Tomohiko Nagaoka, Hidetaka Hori, Ryo Fujimoto & Keiichi Okazaki

  2. Northeast Agricultural University, No. 59 Mucai Street, Xiangfang District, 150030, Haribin, China

    Zi-jing Pu & Yan-ju Zhang

  3. Division of Bioinformatics, National Agricultural Research Center, National Agriculture and Food Research Organization, 3-1-1 Kannondai, Tsukuba, Ibaraki, 305-8666, Japan

    Takeshi Hayashi

  4. National Institute of Vegetable and Tea Science, Ano, Tsu, Mie, 514-2392, Japan

    Satoru Matsumoto

Authors
  1. Zi-jing Pu
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  2. Motoki Shimizu
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  3. Yan-ju Zhang
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  4. Tomohiko Nagaoka
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  5. Takeshi Hayashi
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  6. Hidetaka Hori
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  7. Satoru Matsumoto
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  8. Ryo Fujimoto
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  9. Keiichi Okazaki
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Corresponding author

Correspondence to Keiichi Okazaki.

Additional information

Zi-jing Pu and Motoki Shimizu contributed equally to this work.

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Pu, Zj., Shimizu, M., Zhang, Yj. et al. Genetic mapping of a fusarium wilt resistance gene in Brassica oleracea . Mol Breeding 30, 809–818 (2012). https://doi.org/10.1007/s11032-011-9665-8

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  • DOI: https://doi.org/10.1007/s11032-011-9665-8

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