Skip to main content
SpringerLink
Log in

Mapping quantitative trait loci associated with spot blotch and net blotch resistance in a doubled-haploid barley population

  • Published:
Molecular Breeding Aims and scope Submit manuscript

Abstract

Spot blotch and net blotch are important foliar barley (Hordeum vulgare L.) diseases in Canada and elsewhere. These diseases result in significant yield reduction and, more importantly, loss of grain quality, downgrading barley from malt to feed. Combining resistance to these diseases is a breeding priority but is a significant challenge using conventional breeding methodology. In the present investigation, an evaluation of the inheritance of resistance to spot and net blotch was conducted in a doubled-haploid barley population from the cross CDC Bold (susceptible) × TR251 (resistant). The population was screened at the seedling stage in the Phytotron and at the adult-plant stage in the field for several years. Chi-squared analysis indicated one- to four-gene segregation depending on disease, isolate, plant development stage, location and year. A major seedling and adult-plant resistance quantitative trait locus (QTL), designated QRpt6, was re-confirmed for net-form net blotch resistance, explaining 32–61% of phenotypic variation in different experiments. Additional QTL for seedling and adult-plant resistance to net blotch were identified. For spot blotch resistance, a major seedling resistance QTL (QRcss1) was detected on chromosome 1H for isolate WRS1909, explaining 79% of the phenotypic variation. A highly significant QTL on 3H (QRcs3) was identified for seedling resistance to isolate WRS1908 and adult-plant resistance at Brandon, MB, Canada in 2008. The identification of QTL at only one location or from 1 year suggests spot blotch resistance is complex and highly influenced by the environment. Efforts are being made to combine spot and net blotch resistance in elite barley lines using molecular marker-assisted selection.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Fig. 1
Fig. 2

Similar content being viewed by others

References

  • Abu Qamar M, Liu ZH, Faris JD, Chao S, Edwards MC, Lai Z, Franckowiak JD, Friesen TL (2008) A region of barley chromosome 6H harbours multiple major genes associated with net type net blotch resistance. Theor Appl Genet 117:1261–1270

    Article  PubMed  CAS  Google Scholar 

  • Bilgic H, Steffenson BJ, Hayes PM (2005) Comprehensive genetic analyses reveal differential expression of spot blotch resistance in four populations of barley. Theor Appl Genet 111:1238–1250

    Article  PubMed  CAS  Google Scholar 

  • Bilgic H, Steffenson BJ, Hayes PM (2006) Molecular mapping of loci conferring resistance to different pathotypes of the spot blotch pathogen in barley. Phytopathology 96:699–708

    Article  PubMed  CAS  Google Scholar 

  • Bovill J, Lehmensiek A, Sutherland MW, Platz GJ, Usher T, Frackowiak J, Mace E (2010) Mapping spot blotch resistance genes in four barley populations. Mol Breed 26:653–666

    Article  CAS  Google Scholar 

  • Cakir M, Gupta S, Platz GJ, Ablett GA, Loughman R, Embiri LC, Poulsen D, Li CD, Lance RCM, Galwey NW, Jones MGK, Appels R (2003) Mapping and validation of the genes for resistance to Pyrenophora teres f. teres in barley (Hordeum vulgare L.). Aust J Agric Res 54:1369–1377

    Article  CAS  Google Scholar 

  • Couture L (1980) Assessment of severity of foliage diseases of cereals in cooperative evaluation tests. Can Plant Dis Surv 60:8–10

    Google Scholar 

  • Fetch TG, Steffenson BJ (1999) Rating scales for assessing infection responses of barley infected with Cochliobolus sativus. Plant Dis 83:213–217

    Article  Google Scholar 

  • Friesen TL, Faris JD, Lai Z, Steffenson BJ (2006) Identification and chromosomal location of major genes for resistance to Pyreonophora teres in a doubled-haploid barley population. Genome 49:855–859

    Article  PubMed  CAS  Google Scholar 

  • Ghazvini H, Tekauz A (2007) Virulence diversity in the population of Bipolaris sorokiniana isolates. Plant Dis 91:814–821

    Article  Google Scholar 

  • Grewal TS, Rossnagel BG, Pozniak C, Scoles GJ (2008) Mapping quantitative trait loci associated with barley net blotch resistance. Theor Appl Genet 116:529–539

    Article  PubMed  CAS  Google Scholar 

  • Grewal TS, Rossnagel BG, Scoles GJ (2010) Validation of molecular markers associated with net blotch resistance and their utilization in barley breeding. Crop Sci 50:177–184

    Article  Google Scholar 

  • Harvey BL, Rossnagel BG (1984) Harrington barley. Can J Plant Sci 64:193–194

    Article  Google Scholar 

  • Khan TN, Tekauz A (1982) Occurrence and pathogenicity of Drechslera teres isolates causing spot-type symptoms on barley in Western Australia. Plant Dis 66:423–425

    Article  Google Scholar 

  • Li J, Baga M, Rossnagel BG, Legge WG, Chibbar RN (2008) Identification of quantitative trait loci for β—glucan concentration in barley grain. J Cereal Sci 48:647–655

    Article  CAS  Google Scholar 

  • Ma ZQ, Lapitan NLV, Steffenson B (2004) QTL mapping of net blotch resistance genes in a doubled-haploid population of six-rowed barley. Euphytica 137:291–296

    Article  CAS  Google Scholar 

  • Manninen O, Kalendar R, Robinson J, Schulman AH (2000) Application of BARE-1 retrotransposon markers to the mapping of a major resistance gene for net blotch in barley. Mol Gen Genet 264:325–334

    Article  PubMed  CAS  Google Scholar 

  • Mathre DE (1997) Compendium of barley diseases, 2nd edn. American Phytopathological Society, St. Paul

    Google Scholar 

  • Meldrum SI, Platz GJ, Ogle HJ (2004) Pathotypes of Cochliobolus sativus on barley in Australia. Australas Plant Pathol 33:109–114

    Article  Google Scholar 

  • Nutter FW, Pederson VD, Foster AE (1985) Effect of inoculations with Cochliobolus sativus at specific growth stages on grain yield and quality of malting barley. Crop Sci 25:933–938

    Article  Google Scholar 

  • SAS Institute Inc. (2005) SAS/STAT user’s guide. Version 9.1. SAS Institute, Cary

    Google Scholar 

  • Steffenson BJ (1997) Net blotch. In: Mather DE (ed) Compendium of barley diseases, 2nd edn. American Phytopathological Society, St. Paul, pp 28–31

    Google Scholar 

  • Steffenson BJ, Webster RK (1992) Pathotype diversity of Pyrenophora teres f. teres on barley. Phytopathology 82:170–177

    Article  Google Scholar 

  • Steffenson BJ, Hayes PM, Kleinhofs A (1996) Genetics of seedling and adult plant resistance to net blotch (Pyrenophora teres f. teres) and spot blotch (Cochliobolus sativus) in barley. Theor Appl Genet 92:552–558

    Article  CAS  Google Scholar 

  • Tekauz A (1985) A numerical scale to classify reactions of barley to Pyrenophora teres. Can J Plant Pathol 7:181–183

    Article  Google Scholar 

  • Tekauz A (1990) Characterization and distribution of pathogenic variation in Pyrenophora teres f. teres and P. teres f. maculata from western Canada. Can J Plant Pathol 12:141–148

    Article  Google Scholar 

  • Tekauz A (2000) Evaluation of barley cultivar resistance to Pyrenophora teres using combined seedling and adult-plant reactions. In: Logue S (ed) Proceedings of the 8th international barley genetic symposium, vol 2. 22–27 Oct 2000, Department of Plant Science, University of Adelaide, Adelaide, Australia, pp 182–183

  • Tekauz A, Mueller E (2006) The leaf spot complex of barley in Manitoba, Canada: a 10-year perspective. In: Turkington TK, Orr DD, Xi K (eds) Proceedings of the 3rd international workshop on barley leaf blights 23–27 July 2006, University of Alberta Conference Centre, Edmonton, Alberta, Canada, pp 63–66

  • Valjavec-Gratian M, Steffenson BJ (1997) Pathotypes of Cochliobolus sativus on barley in North Dakota. Plant Dis 81:1275–1278

    Article  Google Scholar 

  • van den Berg CGJ (1988) Epidemiology of Pyrenophora teres and its effect on grain yield of Hordeum vulgare. Ph.D. dissertation, University of Saskatchewan, Saskatoon, Canada

  • van Ooijen JW (2004) MapQTL® 5, Software for the mapping of quantitative trait loci in experimental populations. Kyazma B.V, Wageningen

    Google Scholar 

  • Wilcoxon RD, Rasmusson DC, Miles MR (1990) Development of barley resistant to spot blotch and genetics of resistance. Plant Dis 74:207–210

    Article  Google Scholar 

  • Zhong S, Steffenson BJ (2001) Virulence and molecular diversity in Cochliobolus sativus. Phytopathology 91:469–476

    Article  PubMed  CAS  Google Scholar 

Download references

Acknowledgments

We are grateful to Xiang-min Zhang, Rob Stonehouse and Ramon Arango for technical assistance, to Doug Voth for assistance in field experiments, and to Drs. A. Tekauz and K. Turkington for supplying net blotch and spot blotch isolates. We thank Dr. W. Legge for supplying seed of the CDC Bold/TR251 population. We are grateful to Drs. R. Chibbar and J. Li for sharing mapping data of the CDC Bold/TR251 population. Sincere thanks to J. Tucker and W. Legge for screening in the field at Brandon, MB. The work was funded in part by Saskatchewan Agriculture Development Fund Projects # 20040386 & 20070025 and the Western Grains Research Foundation Check-off for barley breeding.

Author information

Author notes

  1. Tajinder S. Grewal

    Present address: GenServe Laboratories™, Saskatchewan Research Council, 125-15 Innovation Blvd., Saskatoon, SK, S7N 2X8, Canada

Authors and Affiliations

  1. Crop Development Centre/Department of Plant Sciences, University of Saskatchewan, 51 Campus Drive, Saskatoon, SK, S7N 5A8, Canada

    Tajinder S. Grewal, Brian G. Rossnagel & Graham J. Scoles

Authors
  1. Tajinder S. Grewal
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Brian G. Rossnagel
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Graham J. Scoles
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to Tajinder S. Grewal.

Electronic supplementary material

Below is the link to the electronic supplementary material.

11032_2011_9616_MOESM1_ESM.docx

Frequency distribution of 150 DH lines from the CDC Bold/TR251 population screened with (a) Pyrenophora teres f teres isolate WRS858, WRS1607 (b) P. teres f maculata isolates WRS857 and LO233 at the seedling stage in the Phytotron and (c) at the adult-plant stage in the field for NFNB resistance. Supplementary material 1 (DOCX 39 kb)

11032_2011_9616_MOESM2_ESM.docx

Frequency distribution of 164 DH lines of the CDC Bold/TR251 population screened with Cochliobolus sativus isolates WRS1909, WRS1908 and SK1-1 at the seedling stage in the Phytotron. Supplementary material 2 (DOCX 20 kb)

11032_2011_9616_MOESM3_ESM.docx

Multiple-QTL model (MQM) LOD scans of chromosome 1H where a major QTL associated with seedling resistance to WRS1909 was detected for spot blotch resistance. Vertical lines indicate significance threshold for each experiment as estimated from 1000 permutations of the data. Supplementary material 3 (DOCX 147 kb)

11032_2011_9616_MOESM4_ESM.docx

Multiple-QTL model (MQM) LOD scans of chromosome 5H where a major QTL associated with seedling resistance to WRS1909 and SK1-1 was detected for spot blotch resistance. An adult-plant resistance QTL was detected for Saskatoon 2009. Vertical lines indicate significance threshold for each experiment as estimated from 1000 permutations of the data. Supplementary material 4 (DOCX 342 kb)

Supplementary material 5 (DOC 35 kb)

Supplementary material 6 (DOC 33 kb)

Supplementary material 7 (DOC 36 kb)

Supplementary material 8 (DOC 31 kb)

Rights and permissions

Reprints and permissions

About this article

Cite this article

Grewal, T.S., Rossnagel, B.G. & Scoles, G.J. Mapping quantitative trait loci associated with spot blotch and net blotch resistance in a doubled-haploid barley population. Mol Breeding 30, 267–279 (2012). https://doi.org/10.1007/s11032-011-9616-4

Download citation

  • Received:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s11032-011-9616-4

Keywords

Search

Navigation