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Major quantitative trait loci for seminal root morphology of wheat seedlings

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Abstract

Vigorous early root growth at seedling stage has been shown to be important for efficient acquisition of nutrients in wheat (Triticum aestivum L.). Identifying quantitative trait loci (QTL) for early root growth can facilitate the selection of wheat varieties with efficient nutrient use. A recombinant inbred line population derived from two Chinese wheat varieties, Xiaoyan 54 and Jing 411, was grown hydroponically at seedling stage. The maximum root length (MRL), primary root length (PRL), lateral root length (LRL), total root length (TRL), and root tip number (RN) of seminal roots were measured using the WinRHIZO Root Analyser. Numerous QTL for the investigated root traits were detected with QTL numbers varying from two to six, depending on the traits. Among them, two loci had major effects on primary (MRL and PRL) and lateral (LRL and RN) root parameters, respectively. The QTL (namely qTaLRO-B1) between Xgwm210 and Xbarc1138.2 on chromosome 2B explained 68.0 and 59.0% of phenotypic variations in MRL and PRL, respectively; the major QTL between Xgwm570 and Xgwm169.2 on chromosome 6A explained 30.5 and 24.5% of phenotypic variations in LRL and RN, respectively. These two major loci showed linkage with previous reported QTL for yield component and nutrient uptake. Detailed analysis of qTaLRO-B1 indicated that the positive allele of qTaLRO-B1 showed dominance over the negative allele, which showed impairment in primary root elongation. The existence of major QTL for root trait and their linkage with agronomic traits and nutrient uptake will facilitate the design of root morphology for better yield performance and efficient nutrient use.

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Acknowledgments

This research was supported by the National Natural Science Foundation of China (30890133) and the Ministry of Science and Technology of China (2009CB118300 and 2010CB125900).

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Correspondence to Yiping Tong or Aimin Zhang.

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Yongzhe Ren and Xue He contributed equally to this work.

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11032_2011_9605_MOESM1_ESM.ppt

Roots of the parents and selected RILs of the Xiaoyan 54 × Jing 411 RIL population in the first trial. A Roots of the parents Xiaoyan 54 and Jing 411; B roots of the selected RILs. Supplementary material 1 (PPT 326 kb)

Supplementary material 2 (DOC 40 kb)

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Ren, Y., He, X., Liu, D. et al. Major quantitative trait loci for seminal root morphology of wheat seedlings. Mol Breeding 30, 139–148 (2012). https://doi.org/10.1007/s11032-011-9605-7

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  • DOI: https://doi.org/10.1007/s11032-011-9605-7

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