Abstract
Kernel length in rice (Oryza sativa L.) is controlled by various quantitative trait loci of which GS3 is the most important, being responsible for 80–90% of the variation in kernel length. A mutation in the second exon of this gene has been reported to be associated with maximum variations in the kernel length. We have developed a simple PCR-based marker system named DRR-GL which targets the functional nucleotide polymorphism at GS3. This marker system has the advantages that it is easy to use, saves time and cost, and is amenable for large-scale marker-assisted selection for the trait of kernel length. Validation of this marker in a segregating population and 152 rice varieties, which includes 30 elite basmati varieties, reveals its effective co-segregation and association with the traits of kernel length as well as kernel elongation after cooking. We recommend utilization of this simple, low-cost marker system in breeding programs targeted at improvement of key rice grain quality traits, kernel length and kernel elongation.
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The authors are grateful to the Department of Biotechnology, Government of India, for providing funds for carrying out the research work.
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Ramkumar, G., Sivaranjani, A.K.P., Pandey, M.K. et al. Development of a PCR-based SNP marker system for effective selection of kernel length and kernel elongation in rice. Mol Breeding 26, 735–740 (2010). https://doi.org/10.1007/s11032-010-9492-3
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DOI: https://doi.org/10.1007/s11032-010-9492-3