Skip to main content

Advertisement

SpringerLink
Log in

Physical mapping of puroindoline b-2 genes and molecular characterization of a novel variant in durum wheat (Triticum turgidum L.)

  • Published:
Molecular Breeding Aims and scope Submit manuscript

Abstract

The puroindoline genes (Pina and Pinb) are the functional components of the common or bread wheat (Triticum aestivum L.) grain hardness locus that are responsible for kernel texture. In this study, four puroindoline b-2 variants were physically mapped using nulli-tetrosomic lines of bread wheat cultivar Chinese Spring and substitution lines of durum wheat (Triticum turgidum L.) cultivar Langdon. Results indicated that Pinb-2v1 was on 7D of Chinese Spring, Pinb-2v2 on 7B of Chinese Spring, Pinb-2v3 on 7B of Chinese Spring and Langdon, and Pinb-2v4 on 7A of Chinese Spring and Langdon. A new puroindoline b-2 variant, designated Pinb-2v5, was identified at the puroindoline b-2 locus of durum wheat cultivar Langdon, with a difference of only five single nucelotide polymorphisms compared with Pinb-2v4. Sequencing results indicated that, in comparison with the Pinb-2v3 sequence (AM99733 and GQ496618 with one base-pair modification of G to T at 6th position, designated Pinb-2v3a) in bread wheat cultivar Witchta, the coding region of Pinb-2v3 in 12 durum wheat cultivars had a single nucleotide change from T to C at the 311th position, resulting in a corresponding amino acid change from valine to alanine at the 104th position. This new allele was designated Pinb-2v3b. The study of puroindoline b-2 gene polymorphism in CIMMYT and Italian durum wheat germplasm and discovery of a novel puroindoline b-2 variant could provide useful information for further understanding the molecular and genetic basis of kernel hardness and illustrating gene duplication events in wheat.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Subscribe and save

Springer+ Basic
$34.99 /Month
  • Get 10 units per month
  • Download Article/Chapter or eBook
  • 1 Unit = 1 Article or 1 Chapter
  • Cancel anytime
Subscribe now

Buy Now

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Fig. 1
Fig. 2
Fig. 3

Similar content being viewed by others

References

  • Bhave M, Morris CF (2008a) Molecular genetics of puroindolines and related genes: allelic diversity in wheat and other grasses. Plant Mol Biol 66:205–219

    Article  PubMed  CAS  Google Scholar 

  • Bhave M, Morris CF (2008b) Molecular genetics of puroindolines and related genes: regulation of expression, membrane binding properties and applications. Plant Mol Biol 66:221–231

    Article  PubMed  CAS  Google Scholar 

  • Bihan TL, Blochet JE, Desormeaux A, Marion D, Pezolet M (1996) Determination of the secondary structure and conformation of puroindolines by infrared and Raman spectroscopy. Biochemistry 35:12712–12722

    Article  PubMed  Google Scholar 

  • Capparelli R, Borriello G, Giroux MJ, Amoroso MG (2003) Puroindoline a-gene expression is involved in association of puroindolines to starch. Theor Appl Genet 107:1463–1468

    Article  PubMed  CAS  Google Scholar 

  • Chantret N, Salse J, Sabot F, Rahman S, Bellec A, Laubin B, Dubois I, Dossat C, Sourdille P, Joudrier P, Gautier MF, Cattolico L, Beckert M, Aubourg S, Weissenbach J, Caboche M, Bernard M, Leroy P, Chalhoub B (2005) Molecular basis of evolutionary events that shaped the hardness locus in diploid and polyploid wheat species (Triticum and Aegilops). Plant Cell 17:1033–1045

    Article  PubMed  CAS  Google Scholar 

  • Chen M, Wilkinson M, Tosi P, He G, Shewry P (2005) Novel puroindoline and grain softness protein alleles in Aegilops species with the C, D, S, M and U genomes. Theor Appl Genet 111:1159–1166

    Article  PubMed  CAS  Google Scholar 

  • Chen F, He ZH, Xia XC, Xia LQ, Zhang XY, Lillemo M, Morris CF (2006) Molecular and biochemical characterization of puroindoline a and b alleles in Chinese landraces and historical cultivars. Theor Appl Genet 112:400–409

    Article  PubMed  CAS  Google Scholar 

  • Chen F, He ZH, Chen DS, Zhang CL, Zhang Y, Xia XC (2007a) Influence of puroindoline alleles on milling performance and qualities of Chinese noodles, steamed bread and pan bread in spring wheats. J Cereal Sci 45:59–66

    Article  CAS  Google Scholar 

  • Chen F, Yu Y, Xia X, He Z (2007b) Prevalence of a novel puroindoline b allele in Yunnan endemic wheats (Triticum aestivum ssp. Yunnanense King). Euphytica 156:39–46

    Article  CAS  Google Scholar 

  • Chen F, Beecher B, Morris CF (2010a) Physical mapping and a new variant of Puroindoline b-2 genes in wheat. Theor Appl Genet 120:745–751

    Article  PubMed  Google Scholar 

  • Chen F, Zhang FY, Cheng XY, Morris CF, Xu HX, Dong ZD, Zhan KH, He ZH, Xia XC, Cui DQ (2010b) Influence on wheat grain yield traits of puroindoline b-2 gene in bread wheat (Triticum aestivum L.) of Yellow and Huai Valley of China. J Cereal Sci, accepted

  • Gautier MF, Aleman ME, Guirao A, Marion D, Joudrier P (1994) Triticum aestivum puroindolines, two basic cystine-rich seed proteins: cDNA sequence analysis and development gene expression. Plant Mol Biol 25:43–51

    Article  PubMed  CAS  Google Scholar 

  • Giroux MJ, Morris CF (1997) A glycine to serine change in puroindoline b is asssociated with wheat grain hardness and low levels of starch-surface friabilin. Theor Appl Genet 95:857–864

    Article  CAS  Google Scholar 

  • Giroux MJ, Morris CF (1998) Wheat grain hardness results from highly conserved mutations in the friabilin components puroindoline a and b. Proc Natl Acad Sci USA 95:6262–6266

    Article  PubMed  CAS  Google Scholar 

  • Gollan P, Smith K, Bhave M (2007) Gsp-1 genes comprise a multigene family in wheat that exhibits a unique combination of sequence diversity yet conservation. J Cereal Sci 45:184–198

    Article  CAS  Google Scholar 

  • Igrejas G, Leroy P, Charmet G, Gaborit T, Marion D, Branlard G (2002) Mapping QTLs for grain hardness and puroindoline content in wheat (Triticum aestivum L.). Theor Appl Genet 106:19–27

    PubMed  CAS  Google Scholar 

  • Kooijman M, Orsel R, Hessing M, Hamer RJ, Bekkers ACAPA (1997) Spectroscopic characterisation of the lipid-binding properties of wheat puroindolines. J Cereal Sci 26:145–159

    Article  CAS  Google Scholar 

  • Lillemo M, Chen F, Xia XC, William M, Pena RJ, Trethowan R, He ZH (2006) Puroindoline grain hardness alleles in CIMMYT bread wheat. J Cereal Sci 44:86–92

    Article  CAS  Google Scholar 

  • Luo LT, Zhang JR, Yang GX, Li Y, Li KX, He GY (2008) Expression of puroindoline a enhances leaf rust resistance in transgenic tetraploid wheat. Mol Biol Rep 35:195–200

    Article  PubMed  CAS  Google Scholar 

  • Massa AN, Morris CF, Gill BS (2004) Sequence diversity of puroindoline-a, puroindoline-b, and the grain softness protein genes in Aegilops tauschii cross. Crop Sci 44:1808–1816

    Article  CAS  Google Scholar 

  • Mattern PJ, Morris R, Schmidt JW, Johnson VA (1973) Location of genes for kernel properties in the wheat cultivar ‘Cheyenne’ using chromosome substitution lines. In: Proceedings of International Wheat Genetics Symposium, Columbi, Mo., 1–6 August 1973. Edited by Sears ERand Sears LMS. Agricultural Experimental Station, Univeristy of Missouri, Columbia, Mo, pp 703–707

  • McIntosh RA, Devos KM, Dubcovsky J, Rogers WJ, Morris CF, Appels R, Anderson OD (2006) Catalogue of gene symbols for wheat: 2006 supplement, published online at: http://wheat.pw.usda.gov/ggpages/wgc/2006upd.html

  • McIntosh RA, Devos KM, Dubcovsky J, Rogers WJ, Morris CF, Appels R, Somers DJ, Anderson OA (2007) Catalogue of gene symbols for wheat: 2007 Supplement. Ann Wheat Newslett 53: 159–180 (Also published on-line at http://wheat.pw.usda.gov/ggpages/wpc/2007upd.html)

  • Morris CF (2002) Puroindolines: the molecular genetic basis of wheat grain hardness. Plant Mol Biol 48:633–647

    Article  PubMed  CAS  Google Scholar 

  • Morris CF, Bhave M (2008) Reconciliation of D-genome puroindoline allele designations with current DNA sequence data. J Cereal Sci 48:277–287

    Article  CAS  Google Scholar 

  • Ragupathy R, Cloutier S (2008) Genome organization and retrotransposon driven molecular evolution of the endosperm Hardness (Ha) locus in Triticum aestivum cv Glenlea. Mol Genet Genom 280:467–481

    Article  CAS  Google Scholar 

  • Rahman S, Jolly CJ, Skerritt JH, Wallosheck A (1994) Cloning of a wheat 15 kDa grain softness protein (GSP). GSP is a mixture of different puroindoline-like polypeptides. Eur J Biochem 223:917–925

    Article  PubMed  CAS  Google Scholar 

  • Sourdille P, Perretant MR, Charmet G, Leroy P, Gautier MF, Joudrier P, Nelson JC, Sorrells ME, Bernard M (1996) Linkage between RFLP markers and genes affecting kernel hardness in wheat. Theor Appl Genet 93:580–586

    Article  CAS  Google Scholar 

  • Turner AS, Bradburne RP, Fish L, Snape JW (2004) New quantitative trait loci influencing grain texture and protein content in bread wheat. J Cereal Sci 40:51–60

    Article  CAS  Google Scholar 

  • Wilkinson M, Wan Y, Tosi P, Leverington M, Snape J, Mitchel RAC, Shewry PR (2008) Identification and genetic mapping of variant forms of puroindoline b expressed in developing wheat grain. J Cereal Sci 48:722–728

    Article  CAS  Google Scholar 

  • Xia LQ, Chen F, He ZH, Chen XM, Morris CF (2005) Occurrence of puroindoline alleles in Chinese winter wheats. Cereal Chem 82:38–43

    Article  CAS  Google Scholar 

Download references

Acknowledgments

The authors thank Dr. Roberto Tuberosa (Department of Agro-Environmental Science and Technology, University of Bologna) and wheat breeder Mr. Tianmin Shen (Henan Tianmin Seed Co., Ltd, China) for kindly providing the seeds of durum wheat cultivars. This research was financially supported by grants from the National 948 project (2010-Z21), 973 project (2009CB118300) and 863 project (2006AA100102) of China.

Author information

Authors and Affiliations

  1. Department of Agronomy, Henan Agricultural University, 450002, Zhengzhou, China

    F. Chen, H.-X. Xu, F.-Y. Zhang, Z.-D. Dong, K.-H. Zhan, X.-Y. Cheng & D.-Q. Cui

  2. Institute of Crop Science, National Wheat Improvement Center, Chinese Academy of Agricultural Sciences (CAAS), 12 Zhongguancun South Street, 100081, Beijing, China

    X.-C. Xia & Z.-H. He

  3. CIMMYT China Office, c/o CAAS, 12 Zhongguancun South Street, 100081, Beijing, China

    Z.-H. He

  4. The State Key Laboratory of Plant Cell and Chromosomal Engineering, Institute of Genetics and Developmental Biology, Chinese Academy of Sciences (CAS), 100101, Beijing, China

    D.-W. Wang

  5. Henan Agricultural University, 95 Wenhua Road or 63 Nongye Road, 450002, Zhengzhou, China

    F. Chen & D.-Q. Cui

Authors
  1. F. Chen
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. H.-X. Xu
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. F.-Y. Zhang
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. X.-C. Xia
    View author publications

    You can also search for this author in PubMed Google Scholar

  5. Z.-H. He
    View author publications

    You can also search for this author in PubMed Google Scholar

  6. D.-W. Wang
    View author publications

    You can also search for this author in PubMed Google Scholar

  7. Z.-D. Dong
    View author publications

    You can also search for this author in PubMed Google Scholar

  8. K.-H. Zhan
    View author publications

    You can also search for this author in PubMed Google Scholar

  9. X.-Y. Cheng
    View author publications

    You can also search for this author in PubMed Google Scholar

  10. D.-Q. Cui
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding authors

Correspondence to F. Chen or D.-Q. Cui.

Rights and permissions

Reprints and permissions

About this article

Cite this article

Chen, F., Xu, HX., Zhang, FY. et al. Physical mapping of puroindoline b-2 genes and molecular characterization of a novel variant in durum wheat (Triticum turgidum L.). Mol Breeding 28, 153–161 (2011). https://doi.org/10.1007/s11032-010-9469-2

Download citation

  • Received:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s11032-010-9469-2

Keywords

Search

Navigation