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SRAP-based mapping and QTL detection for inflorescence-related traits in chrysanthemum (Dendranthema morifolium)

Molecular Breeding volume 27, pages 11–23 (2011)Cite this article

Abstract

Chrysanthemum (Dendranthema morifolium) is an economically important ornamental species and comprises a large proportion of the flower industry in south-east Asian and European countries. In this study, a segregating population of 142 F1 progeny of the cross between the two chrysanthemum cultivars ‘Yuhualuoying’ and ‘Aoyunhanxiao’ was used to construct two separate genetic linkage maps via a double pseudo-testcross mapping strategy. Genotyping was performed using 500 SRAP primer combinations, of which about 50% were informative. This allowed the definition of 896 SRAP loci, of which about 23% showed some segregation distortion. The ‘Yuhualuoying’ map consisted of 333 testcross markers arranged into 57 linkage groups (LGs). It covered >1,900 cM with a mean inter-marker distance of 6.9 cM. The map constructed from ‘Aoyunhanxiao’ comprised 342 test cross markers arranged into 55 LGs. It spanned nearly 1,900 cM, with a mean inter-marker distance of 6.6 cM. The markers were distributed rather uniformly along both maps. A quantitative trait loci analysis was conducted to investigate the pattern of inheritance of three inflorescence traits. This led to the detection of 12 putative loci at a LOD score >2.5, of which four each specified flower diameter, ray floret layer number, and ray floret length. This study provides molecular mapping information on marker-assisted selection programs for the improvement of multiple traits of interest.

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Acknowledgments

This research was financially supported by the National Nature Science Foundation of China (Grant No. 30871724).

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Authors and Affiliations

  1. College of Horticulture, Nanjing Agricultural University, 210095, Nanjing, China

    Fei Zhang, Sumei Chen, Fadi Chen, Weimin Fang, Yu Chen & Fengtong Li

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  1. Fei Zhang
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  3. Fadi Chen
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Correspondence to Fadi Chen.

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Zhang, F., Chen, S., Chen, F. et al. SRAP-based mapping and QTL detection for inflorescence-related traits in chrysanthemum (Dendranthema morifolium). Mol Breeding 27, 11–23 (2011). https://doi.org/10.1007/s11032-010-9409-1

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