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Molecular Breeding

, Volume 25, Issue 4, pp 581–592 | Cite as

Identification of quantitative trait loci for resistance to powdery mildew in a Spanish barley landrace

  • Cristina Silvar
  • Hichem Dhif
  • Ernesto IgartuaEmail author
  • Doris Kopahnke
  • Maria P. Gracia
  • José M. Lasa
  • Frank Ordon
  • Ana M. Casas
Article

Abstract

The Spanish landrace-derived inbred line SBCC97, together with other lines from the Spanish Barley Core Collection, displays high resistance to powdery mildew, caused by the fungus Blumeria graminis f. sp. hordei. The objective of this study was to map quantitative trait loci (QTLs) for resistance to powdery mildew in a recombinant inbred line population derived from a cross between SBCC97 and the susceptible cultivar ‘Plaisant’. Phenotypic analysis was performed using four B. graminis isolates, and genetic maps were constructed with mainly simple sequence repeat (SSR) markers, following a sequential genotyping strategy. Two major QTLs with large effects were identified on chromosome 7H, and they accounted for up to 45% of the total phenotypic variance. The alleles for resistance at each QTL were contributed by the Spanish parent SBCC97. One locus was mapped to the short arm of chromosome 7HS, and was flanked by the resistance gene analogue (RGA) marker S9202 and the SSR GBM1060. This corresponded to the same chromosomal region in which a major race-specific resistance gene from Hordeum vulgare ssp. spontaneum, designated as mlt, had been identified previously. The second QTL was linked tightly to marker EBmac0755, and it shared its chromosomal location with the qualitative resistance gene Mlf, which has only been described previously in the wild ancestor H. spontaneum. This is the first report of these two QTLs occurring together in cultivated barley, and it paves the way for their use in barley breeding programs that are designed to transfer resistance alleles into elite cultivars.

Keywords

Barley Powdery mildew Disease resistance Quantitative trait locus 

Notes

Acknowledgments

This work was supported by the Spanish Ministry of Education and Research (Project AGL2007-63625), and by the European Regional Development Fund. C.S. holds an I3P contract from CSIC. H.D. was supported by a Masters fellowship from IAMZ-CIHEAM.

Supplementary material

11032_2009_9354_MOESM1_ESM.doc (29 kb)
Supplementary material 1 (DOC 29 kb)

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Copyright information

© Springer Science+Business Media B.V. 2009

Authors and Affiliations

  • Cristina Silvar
    • 1
  • Hichem Dhif
    • 1
  • Ernesto Igartua
    • 1
    Email author
  • Doris Kopahnke
    • 2
  • Maria P. Gracia
    • 1
  • José M. Lasa
    • 1
  • Frank Ordon
    • 2
  • Ana M. Casas
    • 1
  1. 1.Department of Genetics and Plant ProductionAula Dei Experimental Station, CSICZaragozaSpain
  2. 2.Institute for Resistance Research and Stress ToleranceJulius Kühn-InstituteQuedlinburgGermany

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