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Introgression of the low-gossypol seed & high-gossypol plant trait in upland cotton: Analysis of [(Gossypium hirsutum × G. raimondii)² × G. sturtianum] trispecific hybrid and selected derivatives using mapped SSRs

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Abstract

In order to select genotypes of Gossypium hirsutum genetically balanced and expressing the low-gossypol seed & high-gossypol plant trait introgressed from the Australian wild diploid species G. sturtianum, the [(G. hirsutum × G. raimondii)² × G. sturtianum] triple hybrid was backcrossed to G. hirsutum and autopollinated to produce backcross and selfed progenies. Two hundred and six mapped SSR markers of G. hirsutum were used to monitor the introgression of SSR alleles specific to G. sturtianum and G. raimondii in the selected progenies. A high level of heterozygosity, varying from 25 to 100%, was observed for all G. sturtianum-specific SSR markers conserved in the most advanced progenies. These results indicate the existence of segregation distortion factors that are associated with the genes controlling the researched trait. This study represents a starting point to map the genes involved in the expression of the trait and better understand its genetic determinism.

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Acknowledgments

The Ph.D. scholarship of the first author was provided by the Belgian General Direction of International Cooperation for Development.

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Authors and Affiliations

  1. Department of Tropical Crop Husbandry and Horticulture, Department of Analytical Chemistry, Gembloux Agricultural University, Passage des Déportés 2, 5030, Gembloux, Belgium

    H. Benbouza, F. B. H. Diouf, D. Sarr, N. Konan, J. P. Baudoin & G. Mergeai

  2. UMR DAP, TA 70/03, CIRAD, Avenue Agropolis, 34398, Montpellier Cedex 05, France

    J. M. Lacape & B. Courtois

  3. Département de Biotechonologie, Centre Wallon de Recherche agronomique, 5030, Gembloux, Belgium

    J. M. Jacquemin

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  1. H. Benbouza
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  2. J. M. Lacape
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  3. J. M. Jacquemin
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  4. B. Courtois
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  5. F. B. H. Diouf
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  8. J. P. Baudoin
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  9. G. Mergeai
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Correspondence to G. Mergeai.

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Benbouza, H., Lacape, J.M., Jacquemin, J.M. et al. Introgression of the low-gossypol seed & high-gossypol plant trait in upland cotton: Analysis of [(Gossypium hirsutum × G. raimondii)² × G. sturtianum] trispecific hybrid and selected derivatives using mapped SSRs. Mol Breeding 25, 273–286 (2010). https://doi.org/10.1007/s11032-009-9331-6

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