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Molecular Breeding

, Volume 23, Issue 2, pp 299–309 | Cite as

Parental genome composition and genetic classifications of derivatives from intergeneric crosses of Festuca mairei and Lolium perenne

  • Jianping P. Wang
  • Suleiman S. BughraraEmail author
  • Rouf M. A. Mian
  • Malay C. Saha
  • David A. Sleper
Article

Abstract

Intergeneric hybridization between Festuca and Lolium has been a long-term goal of forage and turfgrass breeders to generate improved cultivars by combining stress tolerance of Festuca and rapid establishment of Lolium. However, wide-distance hybridizations usually result in the wild genome being eliminated from the hybrid due to incomplete chromosome pairing and crossovers. In this study, random amplified polymorphic DNA (RAPD) and simple sequence repeat (SSR) markers were used to detect the parental genome composition of F1 hybrids and backcross, generated from crosses between Festuca mairei St. Yves (Fm) and Lolium perenne L. (Lp). Each of the hybrids exhibited integration of Fm and Lp genomes with varying levels of Fm/Lp genome ratios. However, cluster and principle component analyses of the progeny consistently revealed four groups depending on the amount of genome introgression from both parents. The parental genome composition and classifications of intergeneric progeny would be useful for breeding material selection.

Keywords

Festuca mairei Lolium perenne Introgression Hybrids Random amplified polymorphic DNA (RAPD) Simple sequence repeats (SSR) 

Abbreviations

EST

Expressed sequence tag

FA

Festuca arundinacea

Fm

Festuca mairei

FDR

First division restitution

FISH

Fluorescence in situ hybridization

GISH

Genomic in situ hybridization

LGs

Linkage groups

Lp

Lolium perenne

RAPD

Random amplified polymorphic DNA

SAHN

Sequential agglomerative hierarchical nested

SSR

Simple sequence repeats

UPGMA

Unweighted pair-group method with arithmetic mean

Supplementary material

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Copyright information

© Springer Science+Business Media B.V. 2008

Authors and Affiliations

  • Jianping P. Wang
    • 1
  • Suleiman S. Bughrara
    • 1
    Email author
  • Rouf M. A. Mian
    • 2
    • 3
  • Malay C. Saha
    • 2
  • David A. Sleper
    • 4
  1. 1.Department of Crop and Soil SciencesMichigan State UniversityEast LansingUSA
  2. 2.Forage Improvement DivisionThe Samuel Roberts Noble FoundationArdmoreUSA
  3. 3.USDA-ARSWoosterUSA
  4. 4.Division of Plant SciencesUniversity of Missouri-ColumbiaColumbiaUSA

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