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High-resolution mapping and gene prediction of Xanthomonas Oryzae pv. Oryzae resistance gene Xa7

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Abstract

Bacterial blight (BB) caused by Xanthomonas oryzae pv. oryzae (Xoo) is a devastating disease in rice worldwide. The resistance gene Xa7, which provides dominant resistance against the pathogen with avirulence (Avr) gene AvrXa7, has proved to be durably resistant to BB. A set of SSR markers were selected from the “gramene” database based on the Xa7 gene initial mapping region on chromosome 6. These markers were used to construct a high-resolution genetic map of the chromosomal region surrounding the Xa7 gene. An F2 mapping population with 721 highly susceptible individuals derived from a cross between the near isogenic lines (NILs) IRBB7 and IR24 were constructed to localize the Xa7 gene. In a primary analysis with eleven polymorphic SSR markers, Xa7 was located in approximately the 0.28-cM region. To walk closer to the target gene, recombinant F2 individuals were tested using newly developed STMS (sequence tagged microsatellite) markers. Finally, the Xa7 gene was mapped to a 0.21-cM interval between the markers GDSSR02 and RM20593. The Xa7-linked markers were landed on the reference sequence of cv. Nipponbare through bioinformatics analysis. A contig map corresponding to the Xa7 gene was constructed. The target gene was assumed to span an interval of approximately 118.5-kb which contained a total of fourteen genes released by the TIGR Genome Annotation Version 5.0. Candidate-gene analysis of Xa7 revealed that the fourteen genes encode novel domains that have no amino acid sequence similar to other cloned Xa(xa) genes.

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Acknowledgments

This research was supported by grants from the National High Technique Project (2006AA100101), the Guangdong Province Science and technique Project (0711124900076), the Guangdong Provincial Natural Science Foundation (2005B20101006), and the Fundamental Science Foundation of Guangdong Academy of Agricultural Science.

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  1. Zhanghui Huang

    Present address: The Rice Research Institute, Guangdong Academy of Agricultural Sciences, Guangzhou, 510640, China

Authors and Affiliations

  1. The Plant Protection Research Institute, Guangdong Academy of Agricultural Sciences, Guangzhou, 510640, China

    Shen Chen, Liexian Zeng, Jianyuan Yang & Xiaoyuan Zhu

  2. Laboratory of Plant Bacteria, College of Resources and Environmental Sciences, South China Agricultural University, Guangzhou, 510642, China

    Zhanghui Huang & Qiongguang Liu

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  1. Shen Chen
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Correspondence to Xiaoyuan Zhu.

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Shen Chen and Zhanghui Huang are contributed equally to this work.

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Chen, S., Huang, Z., Zeng, L. et al. High-resolution mapping and gene prediction of Xanthomonas Oryzae pv. Oryzae resistance gene Xa7 . Mol Breeding 22, 433–441 (2008). https://doi.org/10.1007/s11032-008-9187-1

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