Molecular Breeding

, Volume 22, Issue 2, pp 201–215 | Cite as

Mapping QTL for climbing ability and component traits in common bean (Phaseolus vulgaris L.)

  • Oscar E. Checa
  • Matthew W. Blair


Common bean (Phaseolus vulgaris L.) varies in growth habit from aggressive climbing types to bush beans. Growth habit is determined by a combination of factors including determinate versus indeterminate growth, total plant height, degree of branching and internode length. Together these factors make up climbing ability. The objective of this research was to determine the quantitative trait loci (QTL) controlling climbing ability in a F5:8 recombinant inbred line population derived from an inter-gene pool cross of an aggressive indeterminate climbing bean with type IV growth habit (G2333) by an indeterminate bush bean of type IIb growth habit (G19839). The population was planted in four randomized complete block design experiments across environments that varied in altitude (from 1,000 to 1,750 masl) and soil fertility (low versus high phosphorus). QTL were identified for plant height, internode length and number of branches per plant on a genetic map covering all common bean linkage groups with a total length of 1,175 cM. In addition a scale was developed to evaluate overall climbing ability and was also used to identify QTL. A total of 7 QTL were found for plant height, 9 for climbing ability, 6 for internode length and 1 for branch number. The largest number and most significant QTL were found on the lower half of linkage group B04 suggesting a major pleiotropic locus for growth habit traits at this location of the genome that is distinct from previously characterized genes which control plant morphology of the crop.


Growth habit Internode length Plant architecture and plant height 



Branch number


Climbing ability


Days after planting


Internode length


Likelihood ratio


Plant height


Quantitative trait loci


Recombinant inbred line



We are grateful to Steve Beebe for population development; to Ivan Ochoa and Hector Fabio Buendía for laboratory collaboration; and to Yercil Viera and Alcides Hincapié for their collaboration in the field. This study was financed by FONTAGRO and the International Center for Tropical Agriculture (CIAT) and was part of a PhD dissertation at the National University of Colombia.


  1. Bassett MJ (1997) Tight linkage between the Fin locus for plant habit and the Z locus for partly colored seed coat patterns in common bean. J Am Soc Hortic Sci 122:656–658Google Scholar
  2. Basten CJ, Weir BS, Zeng ZB (2001) QTL cartographer: a reference manual and tutorial for QTL mapping. Department of Statistics, North Carolina State University, RaleighGoogle Scholar
  3. Blair MW, Pedraza F, Buendia HF, Gaitan E, Beebe SE, Gepts P, Tohme J (2003) Development of a genome-wide, anchored microsatellite map for common bean (Phaseolus vulgaris L). Theor Appl Genet 107:1362–1374PubMedCrossRefGoogle Scholar
  4. Blair MW, Iriarte G, Beebe S (2006) QTL analysis of yield traits in an advanced backcross population derived from a cultivated Andean × wild common bean (Phaseolus vulgaris L.) cross. Theor Appl Genet 112:1149–1163PubMedCrossRefGoogle Scholar
  5. Bliss FA (1971) Inheritance of growth habit and time of flowering in beans Phaseolus vulgaris L. J Am Soc Hortic Sci 93:715–717Google Scholar
  6. Broughton WJ, Hernandez G, Blair M, Beebe S, Gepts P, Vanderleyden J (2003) Beans (Phaseolus spp.)—model food legumes. Plant Soil 252:55–128CrossRefGoogle Scholar
  7. Checa O, Ceballos H, Blair MW (2006) Generation means analysis of climbing ability in common bean (Phaseolus vulgaris L.) J Hered 97:456–465PubMedCrossRefGoogle Scholar
  8. Churchill GA, Doerge RW (1994) Empirical threshold values for quantitative trait mapping. Genetics 138:963–971PubMedGoogle Scholar
  9. Coyne DP (1967) Photoperiodism: inheritance and linkage studies in Phaseolus vulgaris. J Hered 58:313–314Google Scholar
  10. Davis DW, Frazier WA (1966) Inheritance of some growth habit components in certain types of bush lines of Phaseolus vulgaris L. J Am Soc Hortic Sci 88:384–392Google Scholar
  11. Debouck D (1991) Systematics and morphology. In: van Schoonhoven A, Voysest O (eds) Common beans: research for crop improvement. CAB International, Walingford, pp 55–117Google Scholar
  12. Emerson RA (1904) Heredity in bean hybrids. Ann Rep Nebr Agric Exp St 17:33–78Google Scholar
  13. Emerson RA (1916) A genetic study of plant height in Phaseolus vulgaris L. Nebr Agric Exp State Res Bul 7Google Scholar
  14. Evans A (1973) Exploitation of the variability in plant architecture in Phaseolus vulgaris. In: Wall D (ed) Potential of field bean and other food legumes in Latin America. CIAT, Cali, pp 279–286Google Scholar
  15. Evans A (1976) Beans. In: Simmmonds NW (ed) Evolution of crop plants. Longman, London, pp 168–172Google Scholar
  16. Falconer DS (1960) Introduction to quantitative genetics. Ronald Press, New YorkGoogle Scholar
  17. Frazier WA, Baggett JR, Sistrunk WA (1958) Transfer of certain blue lake bean pod characters to bush beans. Proc Am Soc Hortic Sci 71:416–421Google Scholar
  18. Freyre R, Skroch PW, Blondon A, Shirmohadali A, Johnson WC, Llaca V, Nodari RO, Pereira PA, Tsai S, Tohme J, Dron M, Nienhuis J, Vallejos CE, Gepts P (1998) Towards an integrated linkage map of common bean. 4. Development of a core linkage map alignment of RFLP maps. Theor Appl Genet 97:847–856CrossRefGoogle Scholar
  19. Gentry HS (1969) Origin of the common bean, Phaseolus vulgaris L. Econ Bot 23:55–69Google Scholar
  20. Koening R, Gepts P (1989) Segregation and linkage of genes for seed proteins, isozymes and morphological traits in common bean (Phaseolus vulgaris). J Hered 80:455–459Google Scholar
  21. Koinange EMK, Singh AP, Gepts P (1996) Genetic control of domestication syndrome in common bean. Crop Sci 36:1037–1045CrossRefGoogle Scholar
  22. Kolkman JM, Kelly JD (2003) QTL conferring resistance and avoidance to white mold in common bean. Crop Sci 43:539–548CrossRefGoogle Scholar
  23. Kooiman HN (1931) Monograph on the genetics of Phaseolus. Bibliogr Genet 8:195–413Google Scholar
  24. Kornegay J, White JW, Ortiz de la Cruz O (1992) Growth habit and gene pool effect on inheritance of yield in common bean. Euphytica 62:171–180CrossRefGoogle Scholar
  25. Lamprecht H (1947) The inheritance of the slender-type of Phaseolus vulgaris and some other results. Agric Hortique Genetica 5:72–84Google Scholar
  26. McClean PE, Lee RK, Otto C, Gepts P, Bassett MJ (2002) Molecular and phenotypic mapping of genes controlling seed coat pattern and color in common bean (Phaseolus vulgaris L.). J Hered 93:148–152PubMedCrossRefGoogle Scholar
  27. Miklas PN, Kelly JD, Beebe SE, Blair MW (2006) Common bean breeding for resistance against biotic and abiotic stresses. Euphytica 147:105–131CrossRefGoogle Scholar
  28. Norton JB (1915) Inheritance of growth habit in the common bean. Am Nat 49:242–261CrossRefGoogle Scholar
  29. Ochoa IE, Blair MW, Lynch JP (2006) QTL analysis of adventitious root formation in common bean (Phaseolus vulgaris L.) under contrasting phosphorus availability. Crop Sci 46:1609–1621CrossRefGoogle Scholar
  30. Ortega S (1968) Contribución al estudio de la herencia del hábito de crecimiento en Phaseolus vulgaris L. Agron Trop 28:87–115Google Scholar
  31. SAS (1985) SAS user’s guide. Statistics SAS Institute, Inc., CaryGoogle Scholar
  32. Singh SP (1982) A key for identification of different growth habits of Phaseolus vulgaris L. Annu Rep Bean Improv Coop 25:92–95Google Scholar
  33. Singh SP (1991) Breeding for seed yield. In: van Schoonhoven A, Voysest O (eds) Common bean: research for crop improvement. C.A.B. International, Wallingford/CIAT, Cali, pp 383–443Google Scholar
  34. Tar’an B, Michaels T, Pauls P (2002) Genetic mapping of agronomic traits in common bean. Crop Sci 42:544–556CrossRefGoogle Scholar
  35. Vallejos CE, Ney S, Sakiyama NS, Chase DC (1992) A molecular marker based linkage map of Phaseolus vulgaris L. Genetics 131:733–740PubMedGoogle Scholar
  36. Woolley J, Lépiz R, Portes TA, Voss J (1991) Bean cropping systems. In: Schoonhoven A, Voysest O (eds) Common beans: research for crop improvement. CAB International, Walingford, pp 707–730Google Scholar
  37. Yarnell SH (1965) Cytogenetics of vegetable crops IV. Legumes Bot Rev 31:347–330Google Scholar

Copyright information

© Springer Science+Business Media B.V. 2008

Authors and Affiliations

  1. 1.Faculty of Agricultural SciencesUniversidad de NariñoPastoColombia
  2. 2.Bean ProjectCentro Internacional de Agricultura Tropical (CIAT)CaliColombia

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