Skip to main content

Advertisement

SpringerLink
Log in

Identification and validation of RAPD and SCAR markers linked to the gene Er3 conferring resistance to Erysiphe pisi DC in pea

  • Published:
Molecular Breeding Aims and scope Submit manuscript

Abstract

Three genes, er1, er2 and Er3, conferring resistance to powdery mildew (Erysiphe pisi) in pea have been described so far. Because single gene-controlled resistance tends to be overcome by evolution of pathogen virulence, accumulation of several resistance genes into a single cultivar should enhance the durability of the resistance. Molecular markers linked to genes controlling resistance to E. pisi may facilitate gene pyramiding in pea breeding programs. Molecular markers linked to er1 and er2 are available. In the present study, molecular markers linked to Er3 have been obtained. A segregating F2 population derived from the cross between a breeding line carrying the Er3 gene, and the susceptible cultivar ‘Messire’ was developed and genotyped. Bulk Segregant Analysis (BSA) was used to identify Random Amplified Polymorphic DNA (RAPD) markers linked to Er3. Four RAPD markers linked in coupling phase (OPW04_637, OPC04_640, OPF14_1103, and OPAH06_539) and two in repulsion phase (OPAB01_874 and OPAG05_1240), were identified. Two of these, flanking Er3, were converted to Sequence Characterized Amplified Region (SCAR) markers. The SCAR marker SCW4637 co-segregated with the resistant gene, allowing the detection of all the resistant individuals. The SCAR marker SCAB1874, in repulsion phase with Er3, was located at 2.8 cM from the gene and, in combination with SCW4637, was capable to distinguish homozygous resistant individuals from heterozygous with a high efficiency. In addition, the validation for polymorphism in different genetic backgrounds and advanced breeding material confirmed the utility of both markers in marker-assisted selection.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Subscribe and save

Springer+ Basic
$34.99 /Month
  • Get 10 units per month
  • Download Article/Chapter or eBook
  • 1 Unit = 1 Article or 1 Chapter
  • Cancel anytime
Subscribe now

Buy Now

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Fig. 1
Fig. 2
Fig. 3

Similar content being viewed by others

References

  • Alzate-Marin AL, Nenarim H, Assis De Carvalho G, de Paula Junior TJ, Goncalves de Barros E, Alves Moreira M (1999) Improved selection with newly identified RAPD markers linked to resistance gene to four pathotypes of Colletotrichum lindemuthianum in common bean. Genet Resist 89:281–285

    CAS  Google Scholar 

  • Avila CM, Sillero JC, Rubiales D, Moreno MT, Torres AM (2003) Identification of RAPD markers linked to the Uvf-1 gene conferring hypersensitive resistance against rust (Uromyces viciae-fabae) in Vicia faba L. Theor Appl Genet 107:353–358

    Article  PubMed  CAS  Google Scholar 

  • Ek M, Eklund M, von Post R, Dayteg C, Henriksson T, Weibull P, Ceptilis A, Issac P, Tuvesson S (2005) Microsatellite markers for powdery mildew resistance in pea (Pisum sativum L.). Hereditas 142:86–91

    Article  PubMed  CAS  Google Scholar 

  • FAOSTAT data (2005) http://faostat.fao.org/

  • Fondevilla S, Carver TLW, Moreno MT, Rubiales D (2006) Macroscopic and histological characterisation of genes er1 and er2 for powdery mildew resistance in pea. Eur J Plant Pathol 115:309–321

    Article  Google Scholar 

  • Fondevilla S, Carver TLW, Moreno MT, Rubiales D (2007a) Identification and characterisation of sources of resistance to Erysiphe pisi Syd. in Pisum spp. Plant Breed 126:113–119

    Article  Google Scholar 

  • Fondevilla S, Torres AM, Moreno MT, Rubiales D (2007b) Identification of a new gene for resistance to powdery mildew in Pisum fulvum, a wild relative of pea. Breed Sci 57:181–184

    Article  Google Scholar 

  • Haley SD, Afanador LK, Kelly JD (1994) Selection of monogenic pest resistance traits with coupling- and repulsion-phase RAPDs markers. Crop Sci 34:1061–1066

    Article  Google Scholar 

  • Heringa RJ, Van Norel A, Tazelaar MF (1969) Resistance to powdery mildew (Erysiphe polygoni D.C.) in peas (Pisum sativum L.). Euphytica 18:163–169

    Google Scholar 

  • Hu XY, Ohm HW, Dweikat I (1997) Identification of RAPDs markers linked to the gene PM1 for resistance to powdery mildew in wheat. Theor Appl Genet 94:832–840

    Article  CAS  Google Scholar 

  • Janila P, Sharma B (2004) RAPD and SCAR markers for powdery mildew resistance gene er in pea. Plant Breed 123:271–274

    Article  CAS  Google Scholar 

  • Johnson E, Miklas PN, Stavely JR, Martinez-Cruzado JC (1995) RAPDs for markers-assisted selection of PI 181996 rust resistance in common bean. Theor Appl Genet 90:659–664

    Article  Google Scholar 

  • Kosambi DD (1994) The estimation of map distance from recombination values. Ann Eugen 12:172–175

    Google Scholar 

  • Lander ES, Green P, Abramson J, Barlow A, Dali MJ, Lincoln DE, Newburg L (1987) MAPMAKER: an interactive computer program for constructing genetic linkage maps of experimental and natural populations. Genomics 1:174–181

    Article  PubMed  CAS  Google Scholar 

  • Lu YH, Melero-Vara JM, García-Tejada JA, Blanchard P (2000) Development of SCAR markers linked to the gene Or5 conferring resistance to broomrape (Orobanche cumana Wallr.) in sunflower. Theor Appl Genet 100:625–632

    Article  CAS  Google Scholar 

  • Michelmore R, Paran I, Keselli V (1991) Identification of markers linked to disease-resistance genes by bulked segregant analysis: a rapid method to detect markers in specific genomic regions using segregating populations. Proc Natl Acad Sci USA 88:9828–9832

    Article  PubMed  CAS  Google Scholar 

  • Munjal RL, Chenulu VV, Hora TS (1963) Assessment of losses due to powdery mildew (Erysiphe polygoni) on pea. Indian Phytopathol 19:260–267

    Google Scholar 

  • Rubiales D, Brown JKM, Martín A (1993) Hordeum chilense resistance to powdery mildew and its potential use in cereal breeding. Euphytica 67:215–220

    Article  Google Scholar 

  • Tanksley SD, Young ND, Paterson AH, Bonierbale MW (1989) RFLP mapping in plant breeding: new tools for old sciences. Biotechnology 7:257–264

    Article  CAS  Google Scholar 

  • Timmerman-Vaughan GM, Frew TJ, Weeden NF (1994) Linkage analysis of er1, a recessive Pisum sativum gene for resistance to powdery mildew fungus (Erysiphe pisi D.C). Theor Appl Genet 88:1050–1055

    Article  Google Scholar 

  • Tiwari KR, Penner GA, Warkentin TD, Rashid KY (1997a) Pathogenic variation in Erysiphe pisi, the causal organism of powdery mildew of pea. Can J Plant Pathol 19:267–271

    Article  Google Scholar 

  • Tiwari KR, Penner GA, Warkentin TD (1997b) Inheritance of powdery mildew resistance in pea. Can J Plant Sci 77:307–310

    Google Scholar 

  • Tiwari KR, Penner GA, Warkentin TD (1998) Identification of coupling and repulsion phase RAPD markers for powdery mildew resistance gene er1 in pea. Genome 41:440–444

    Article  CAS  Google Scholar 

  • Tiwari KR, Penner GA, Warkentin TD (1999) Identification of AFLP markers for powdery mildew resistance gene er2 in pea. Pisum Genet 31:27–29

    Google Scholar 

  • Torres AM, Weeden NF, Martín A (1993) Linkage among isozyme, RFLP and RAPD markers in Vicia faba. Theor Appl Genet 85:937–945

    Article  CAS  Google Scholar 

  • Warkentin TD, Rashid KY, Xue AG (1996) Fungicidal control of powdery mildew in field pea. Can J Plant Sci 76:933–935

    CAS  Google Scholar 

  • Williams JGK, Kubelic AR, Livak KJ, Raflaski JA, Tingey SV (1990) DNA polymorphism amplified by arbitrary primers are useful as genetics markers. Nucleic Acids Res 18:6531–6535

    Article  PubMed  CAS  Google Scholar 

  • Young R, Kelly JD (1997) RAPD markers linked to three major anthracnose resistance genes in common bean. Crop Sci 37:940–946

    Article  CAS  Google Scholar 

Download references

Acknowledgments

We thank INIA for providing a grant to the senior author and projects FP6-FOOD-CT-2004-506223 and AGL2005-10181 for financial support.

Author information

Authors and Affiliations

  1. Área de Mejora y Biotecnología, IFAPA, Centro Alameda del Obispo, Apdo. 3092, 14080, Cordoba, Spain

    S. Fondevilla, M. T. Moreno & A. M. Torres

  2. CSIC, Institute of Sustainable Agriculture, Apdo. 4084, 14080, Cordoba, Spain

    D. Rubiales

Authors
  1. S. Fondevilla
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. D. Rubiales
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. M. T. Moreno
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. A. M. Torres
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to S. Fondevilla.

Rights and permissions

Reprints and permissions

About this article

Cite this article

Fondevilla, S., Rubiales, D., Moreno, M.T. et al. Identification and validation of RAPD and SCAR markers linked to the gene Er3 conferring resistance to Erysiphe pisi DC in pea. Mol Breeding 22, 193–200 (2008). https://doi.org/10.1007/s11032-008-9166-6

Download citation

  • Received:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s11032-008-9166-6

Keywords

Search

Navigation