Abstract
A key challenge in marker-assisted selection (MAS) for molecular plant breeding is to develop markers linked to genes of interest which are applicable to multiple breeding populations. In this study representative F2 plants from a cross Mandalup (resistant to anthracnose disease) × Quilinock (susceptible) of Lupinus angustifolius were used in DNA fingerprinting by Microsatellite-anchored Fragment Length Polymorphism (MFLP). Nine candidate MFLP markers linked to anthracnose resistance were identified, then ‘validated’ on 17 commercial cultivars. The number of “false positives” (showing resistant-allele band but lack of the R gene) for each of the nine candidate MFLP markers on the 17 cultivars ranged from 1 to 9. The candidate marker with least number of false positive was selected, sequenced, and was converted into a co-dominant, sequence-specific, simple PCR based marker suitable for routine implementation. Testing on 180 F2 plants confirmed that the converted marker was linked to the R gene at 5.1 centiMorgan. The banding pattern of the converted marker was consistent with the disease phenotype on 23 out of the 24 cultivars. This marker, designated “AnManM1”, is now being used for MAS in the Australian lupin breeding program. We conclude that generation of multiple candidate markers, followed by a validation step to select the best marker before conversion to an implementable form is an efficient strategy to ensure wide applicability for MAS.
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Buirchell BJ, Sweetingham MW (2006) Invited paper: lupin genetic improvement for targeted environments and markets. In: Ground-breaking stuff. Proceedings of the 13th Australian Agronomy Conference, 10–14 September 2006, Perth, Western Australia. Australian Society of Agronomy. ISBN 1 920842 31 4
Boersma JG, Pollotta M, Li C, Buirchell BJ, Sivasithamparam K, Yang H (2005) Construction of a genetic linkage map using MFLP, and identification of molecular markers linked to domestication genes in narrow-leafed lupin (Lupinus angustifolius). Cell Mol Biol Lett 10:331–344
Clements JC, Buirchell BJ, Yang H, Smith PMC, Sweetingham MW, Smith CG (2005) Chapter 9, Lupin. In: Singh RJ, Jauhar PP (eds) Genetic resources, chromosome engineering, and crop improvement, vol 1, grain legumes. CRC Press, Florida, USA, pp 231–323
Eagles HA, Bariana HS, Ogbonnaya FC, Rebetzke GJ, Hollamby GJ, Henry RJ, Henschke PH, Carter M (2001) Implementation of markers in Australian wheat breeding. Aust J Agric Res 52:1349–1356
Gupta PK, Varshney RK, Sharma PC, Ramesh B (1999) Molecular markers and their applications in wheat breeding. Plant Breed 118:369–390
Holland JB (2004) Implementation of molecular markers for quantitative traits in breeding programs—challenges and opportunities. Proceedings of the 4th International Crop Science Congress, 26 Sep–1 Oct 2004. Brisbane, Australia (http://www.cropscience.org.au)
Johnson ES, Wolff MF, Wernsman EA (2002) Marker-assisted selection for resistance to black shank disease in tobacco. Plant Dis 86:1303–1309
Koebner R, Summers R (2002) The impact of molecular markers on the wheat breeding paradigm. Cell Mol Biol Lett 7:695–702
Manly KF, Cudmore Jr RH, Meer JM (2001) MapManager QTX, cross-platform software for genetic mapping. Mamm Genome 12:930–932
Michelmore RW, Paran I, Kesseli RV (1991) Identification of markers linked to disease-resistance genes by bulked segregant analysis: a rapid method to detect markers in specific gemonic regions by using segregating populations. Proc Natl Acad Sci USA 88:9828–9832
Molnar SJ, James LE, Kasha KJ (2000) Inheritance and RAPD tagging of multiple genes for resistance to net blotch in barley. Genome 43:223–231
Nirenberg HI, Feiler U, Hagedorn G (2002) Description of Colletotrichum lupini comb. Nov. in modern terms. Mycologia 94:307–320
Ogbonnaya FC, Moullet O, Eastwood RF, Lollmorgen J, Eagles H, Appels R, Lagudah ES (1998) The use of molecular markers to pyramid cereal cyst nematode resistance genes in wheat. Proceedings 9th International Wheat Genetics Symposium, vol 3. Saslotoon, August 1998, pp 138–139. University Extension Press, Saskatoon, Saskatchewan
Ogbonnaya FC, Subrahmanyam NC, Moullet O, de Majnik J, Eagles HA, Brown JS, Eastwood RF, Kollmorgen J, Appels R, Lagudah ES (2001) Diagnostic DNA markers for cereal cyst nematode resistance in bread wheat. Aust J Agric Res 52:1367–1374
Shankar M, Sweetingham MW, Cowling WA (2002) Identification of alleles at two loci controlling resistance to Phomopsis stem blight in narrow-leafed lupin (Lupinus angustifolius L.). Euphytica 125:35–44
Sharp PJ, Johnston S, Brown G, McIntosh RA, Pallotta M, Carter M, Bariana HS, Khatkar S, Lagudah ES, Singh RP, Khairallah M, Potter R, Jones MGK (2001) Validation of molecular markers for wheat breeding. Aust J Agric Res 52:1357–1366
Snape JW (2004) Challenges of integrating conventional breeding and biotechnology: a personal view! Proceedings of the 4th International Crop Science Congress, 26 Sep–1 Oct 2004. Brisbane, Australia (http://www.cropscience.org.au)
Staub JE, Serquen FC (1996) Genetic markers, map construction, and their application in plant breeding. HortScience 31:729–740
Thomas GJ, Sweetingham MW (2004) Cultivar and environment influence the development of lupin anthracnose caused by Colletotrichum lupini. Australas Plant Pathol 33:571–577
Vos P, Hogers R, Bleeker M, Reijans M, Lee T, Hornes M, Frijters A, Pot J, Peleman J, Kuiper M, Zabeau M (1995) AFLP: a new technique for DNA fingerprinting. Nucleic Acids Res 23:4407–4414
Wu K, Jones R, Danneberger L, Scolnik PA (1994) Detection of microsatellite polymorphisms without cloning. Nucleic Acids Res 22:3257–3258
Yang H, Sweetingham MW (1998) The taxonomy of Colletotrichum isolates associated with lupin anthracnose. Aust J Agric Res 49:1213–1223
Yang H, Sweetingham MW, Cowling WA, Smith PMC (2001) DNA fingerprinting based on microsatellite-anchored fragment length polymorphisms, and isolation of sequence-specific PCR markers in lupin (Lupinus angustifolius L.). Mol Breed 7:203–209
Yang H, Shankar M, Buirchell BJ, Sweetingham MW, Caminero C, Smith PMC (2002) Development of molecular markers using MFLP linked to a gene conferring resistance to Diaporthe toxica in narrow-leafed lupin (Lupinus angustifolius L.). Theor Appl Genet 105:265–270
Yang H, Boersma JG, You M, Buirchell BJ, Sweetingham MW (2004) Development and implementation of a sequence-specific PCR marker linked to a gene conferring resistance to anthracnose disease in narrow-leafed lupin (Lupinus angustifolius L.). Mol Breed 14:145–151
You M, Boersma JG, Buirchell BJ, Sweetingham MW, Siddique KHM, Yang H (2005) A PCR-based molecular marker applicable for marker-assisted selection for anthracnose disease resistance in lupin breeding. Cell Mol Biol Lett 10:123–134
Young ND (1999) A cautiously optimistic vision for marker-assisted breeding. Mol Breed 5:505–510
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This research was supported by the Grain Research and Development Corporation (Australia) project DAW0102.
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Yang, H., Renshaw, D., Thomas, G. et al. A strategy to develop molecular markers applicable to a wide range of crosses for marker assisted selection in plant breeding: a case study on anthracnose disease resistance in lupin (Lupinus angustifolius L.). Mol Breeding 21, 473–483 (2008). https://doi.org/10.1007/s11032-007-9146-2
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DOI: https://doi.org/10.1007/s11032-007-9146-2