We identified a set of sound-responsive genes in plants using a sound-treated subtractive library and demonstrated sound regulation through mRNA expression analyses. Under both light and dark conditions, sound up-regulated expression of rbcS and ald. These are also light-responsive genes and these results suggest that sound could represent an alternative to light as a gene regulator. Ald mRNA expression increased significantly with treatment at 125 and 250 Hz, whereas levels decreased significantly with treatment at 50 Hz, indicating a frequency-specific response. To investigate whether the ald promoter responds to sound, we generated transgenic rice plants harboring a chimeric gene comprising a fusion of the ald promoter and GUS reporter. In three independent transgenic lines treated with 50 or 250 Hz for 4 h, GUS mRNA expression was up-regulated at 250 Hz, but down-regulated at 50 Hz. Thus, the sound-responsive mRNA expression pattern observed for the ald promoter correlated closely with that of ald, suggesting that the 1,506 bp ald promoter is sound-responsive. Therefore, we propose that in transgenic plants, specific frequencies of sound treatment could be used to regulate the expression of any gene fused to the ald promoter.
This is a preview of subscription content, log in to check access.
Buy single article
Instant access to the full article PDF.
Price includes VAT for USA
Subscribe to journal
Immediate online access to all issues from 2019. Subscription will auto renew annually.
This is the net price. Taxes to be calculated in checkout.
Bhagyalakshmi A, Berthiaume F, Reich KM, Frangos JA (1992) Fluid shear stress stimulates membrane phospholipid metabolism in cultured human endothelial cells. J Vasc Res 29(6):443–449
Bogre L, Ligterink W, Meskiene I, Barker PJ, Heberle-Bors E, Husisson NS, Hirt H (1996) Wounding induces the rapid and transient activation of a specific MAP kinase pathway. Plant Cell 9:75–83
Bouch W, Jiping S, Biao L, Jie L, Chuanren D (2004) Soundwave stimulation triggers the content change of the endogenous hormone of the Chrysanthemum mature callus. Colloids Surf 37:107–112
Braam J (1992) Regulated expression of the calmodulin-related TCH genes in cultures Arabidopsis cells: induction by calcium and heat shock. PNAS 80:3212–3216
Braam J (2005) In touch: plant responses to mechanical stimuli. New Physiol 165:373–389
Braam J, Davis RW (1990) Rain-, wind-, and touch-induced expression of calmodulin and calmodulin-related genes in Arabidopsis. Cell 60:357–364
Braam J, Sistrunk ML, Sistrunk DH, Xu W, Purugganan MM, Antosiewicz DM, Campbell P, Johnson KA (1977) Plant responses to environmental stress: regulation and function of the Arabidopsis TCH genes. Planta 203:S35–S41
Creath K, Schwartz GE (2004) Measuring effects of music, noise, and healing energy using a seed germination bioassay. J Altern Complement Med 10(1):113–122
Galston AW, Slayman CL (1979) The not-so-secret life of plants. Am Sci 67:337–344
Hiei Y, Komari S, Kumashiro T (1994) Efficient transformation of rice (Oryza sativa L.) mediated by Agrobacterium and sequence analysis of the boundaries of the T-DNA. Plant J 6:271–282
Hofgen R, Willmitzer L (1988) Storage of competent cells for Agrobacterium transformation. Nucleic Acids Res 16:9877
Hsieh HJ, Frangos JA (1991) Shear stress increases endothelial platelet-derived growth factor mRNA levels. Am J Physiol 260:H642–646
Hsieh HJ, Li NQ, Frangos JA (1993) Pulsatile and steady flow induces c-fos expression in human endothelial cells. J Cell Physiol 154:143–151
Johnson KA, Sistrunk ML, Polisensky DH, Braam J (1998) Arabidopsis thaliana responses to mechanical stimulation do not required ETR1 or EIN2. Plant Physiol 116:643–649
Knight MR, Campbell AK, Smith SM, Trewavas AJ (1992) Transgenic plant aequorin reports the effects of touch and cold-shock and elicitors on cytoplasmic calcium. Nature (London) 352:524–526
Mizoguchi T, Gotoh Y, Nishida E, Yamaguchi-Shinozaki K, Hayashida N, Iwasaki T, Kamada T, Shinozaki K (1994) Characterization of two cDNAs that encode MAP kinase homologues in Arabidopsis thaliana and analysis of the possible role of auxin in activating such kinase activities in cultured cells. Plant J 5:111–122
Mizoguchi T, Hayashida N, Yamaguchi-Shinozaki K, Matsumoto K, Shinozaki K (1995) Two genes that encode ribosomal-protein S6 kinase homologs are induced by cold or salinity stress in Arabidopsis thaliana. FEBS Lett 358:199–204
Mizoguchi T, Irie K, Hirayama T, Hayashida N, Yamaguchi-Shinozaki K, Matsumoto K, Shinozaki K (1996) A gene encoding a mitogen-activated protein kinase is induced simultaneously with genes for a mitogen-activated protein kinase and an S6 ribosomal protein kinase by touch, cold, and water stress in Arabidopsis thaliana. PNAS 93:765–769
Reich KM, Gay CV, Frangos JA (1990) Fluid shear stress as a mediator of osteoblast cyclic adenosine monophosphate production. Cell Physiol 143(1):100–104
Sambrook J, Fritsch EF, Maniatis T (1989) Molecular cloning: a laboratory manual, 2nd edn. Cold Spring Harbor Laboratory Press, Cold Spring Harbor, NY, USA
Sistrunk ML, Antosiewicz DM, Purugganan MM, Braam J (1994) Arabidopsis TCH3 encodes a novel Ca2+ binding protein and shows environmentally induced and tissue-specific regulation. Plant Cell 6:1553–1565
Takakashi H, Suge H, Kato T (1992) Growth promotion by vibration at 50 Hz in rice and cucumber seedlings. Plant Cell Physiol 32:729–732
Weinberger P, Burton C (1981) The effect of sonication on the growth of some tree seeds. Can J Forest Res 11:840–844
Weinberger P, Das G (1972) The effect of an audible and low ultrasound frequency on the growth of synchronized cultures of Scenedesmus obtusiculus. Can J Bot 50:361–366
Weinberger P, Measures M (1978) Effects of the intensity of audible sound on the growth and development of Rideau winter wheat. Can J Bot 57:1036–1039
Woodhead M, Davies HV, Brennan RM, Taylor MA (1998) The isolation of genomic DNA from blackcurrant (Ribes nigrum L.). Mol Biotechnol 9:243–246
Yoshida S, Forno DA, Cook JH, Gomez KA (1976) Laboratory manual for physiological studies of rice. International Rice Research Institute, Philippines, pp 61–66
This study was financially supported by the Crop Functional Genomics Project of the 21C Frontier Program governed by the Ministry of Science and Technology and Rural Development Administration (RDA), and by the Biogreen21 Program of the RDA.
About this article
Cite this article
Jeong, M., Shim, C., Lee, J. et al. Plant gene responses to frequency-specific sound signals. Mol Breeding 21, 217–226 (2008). https://doi.org/10.1007/s11032-007-9122-x
- Frequency-specific expression
- Sound-induced genes