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Maternal Inheritance of Chloroplasts between Brassica rapa and F1-hybrids Demonstrated by cpDNA Markers Specific to Oilseed Rape and B. rapa

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Abstract

Controlled reciprocal crosses between B. rapa and F1-hybrids (B. napus (♀) ×  B. rapa), giving 20 pair-crossings, were made to reveal possible irregularities in chloroplast inheritance during production of BC1s. Despite the close relationship of chloroplasts in B. rapa and B. napus, development of PCR-based molecular markers specific to B. rapa chloroplasts and B. napus chloroplasts was successful. Offspring from each cross were investigated and among these, we found no irregular chloroplast inheritance, since their plastid genotypes in all cases were identical to that of their mother. With a certainty of 95% our data indicate that the probability that chloroplasts are being inherited paternally is less than 0.015. In oilseed rape, pollen-mediated transgene-dispersal poses a well-known risk. Our results support development of transplastomic oilseed rape as an approach to reduce transgene dispersal.

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References

  • F.J. Belzile (2002) ArticleTitleTransgenic, transplastomic and othergenetically modified plants: a Canadian perspective Biochimie 84 1111–1118 Occurrence Handle10.1016/S0300-9084(02)00018-4 Occurrence Handle12595139 Occurrence Handle1:CAS:528:DC%2BD3sXht1Kns70%3D

    Article  PubMed  CAS  Google Scholar 

  • J.D. Bewley F.D. Hempel S. McCormick P. Zambryski (2000) Reproductive development B.B. Buchanan W. Gruissem R.L. Jones (Eds) Biochemistry & Molecular Biology of Plants The American Society of Plant Physiologists USA 988–1043

    Google Scholar 

  • R. Bock (2001) ArticleTitleTransgenic plastids in basic research and plant biotechnology J. Mol. Biol. 312 425–438 Occurrence Handle10.1006/jmbi.2001.4960 Occurrence Handle11563907 Occurrence Handle1:CAS:528:DC%2BD3MXmvV2isrs%3D

    Article  PubMed  CAS  Google Scholar 

  • B.P. Carlin T.A. Louis (1996) Bayes and Empirical Bayes Methods for Data Analysis Chapman & Hall London

    Google Scholar 

  • D. Chamberlain C.N. Stewart (1999) ArticleTitleTransgene escape and transplastomics Nat. Biotechnol. 17 330–331 Occurrence Handle10.1038/7882 Occurrence Handle10207875 Occurrence Handle1:CAS:528:DyaK1MXitl2itLo%3D

    Article  PubMed  CAS  Google Scholar 

  • Y.M. Charters A. Robertson M.J. Wilkinson G. Ramsay (1996) ArticleTitlePCR analysis of oilseed rape cultivars (Brassica napus L. ssp oleifera) using 5′-anchored simple sequence repeat(SSR) primers Theor. Appl. Genet. 92 442–447 Occurrence Handle1:CAS:528:DyaK28XltFOjtLs%3D

    CAS  Google Scholar 

  • J.L. Corriveau A.W. Coleman (1988) ArticleTitleRapid screening method to detect potential biparental inheritance of plastid DNA and results for over 200 angiosperm species Am. J. Bot. 75 1443–1458

    Google Scholar 

  • H. Daniell M.S. Khan L. Allison (2002) ArticleTitleMilestones inchloroplast genetic engineering: an environmentally friendly era in biotechnology Trends Plant Sci. 7 84–91 Occurrence Handle10.1016/S1360-1385(01)02193-8 Occurrence Handle11832280 Occurrence Handle1:CAS:528:DC%2BD38XhvFyku7s%3D

    Article  PubMed  CAS  Google Scholar 

  • H. Daniell B. Muthukumar S.B. Lee (2001) ArticleTitleMarker free transgenic plants: engineering the chloroplast genome without the use of antibiotic selection Curr. Genet. 39 109–116 Occurrence Handle10.1007/s002940100185 Occurrence Handle11405095 Occurrence Handle1:CAS:528:DC%2BD3MXivFais7w%3D

    Article  PubMed  CAS  Google Scholar 

  • B. Cosa ParticleDe W. Moar S.B. Lee M. Miller H. Daniell (2001) ArticleTitleOverexpression of the Bt cry2Aa2 operon in chloroplasts leads to formation of insecticidal crystals Nat. Biotechnol. 19 71–74 Occurrence Handle11135556

    PubMed  Google Scholar 

  • L.B. Hansen H.R. Siegismund R.B. Jørgensen (2001) ArticleTitleIntrogression between oilseed rape (Brassica napus L.) and its weedy relative B. rapa L in a natural population Genet. Resour. Crop Evol. 48 621–627 Occurrence Handle10.1023/A:1013825816443

    Article  Google Scholar 

  • L.B. Hansen H.R. Siegismund R.B. Jørgensen (2003) ArticleTitleProgressive introgression between Brassica napus (oilseed rape) and B. rapa Heredity 91 276–283 Occurrence Handle10.1038/sj.hdy.6800335 Occurrence Handle12939629 Occurrence Handle1:CAS:528:DC%2BD3sXms1Cqs7s%3D

    Article  PubMed  CAS  Google Scholar 

  • R. Haygood A.R. Ives D.A. Andow (2004) ArticleTitlePopulation genetics of transgene containment Ecol. Lett. 7 213–220 Occurrence Handle10.1111/j.1461-0248.2004.00575.x

    Article  Google Scholar 

  • B.K. Hou Y.H. Zhou L.H. Wan Z.L. Zhang G.F. Shen Z.H. Chen Z.M. Hu (2003) ArticleTitleChloroplast transformation in oilseed rape Transgenic Res. 12 111–114 Occurrence Handle10.1023/A:1022180315462 Occurrence Handle12650529 Occurrence Handle1:CAS:528:DC%2BD3sXnsFamtQ%3D%3D

    Article  PubMed  CAS  Google Scholar 

  • M.M. Johannessen T.N. Mikkelsen R.B. Jørgensen (2002) ArticleTitleCO2exploitation and genetic diversity in winter varieties of oilssed rape (Brassica napus); varieties of tomorrow Euphytica 128 75–86

    Google Scholar 

  • R.B. Jørgensen B. Andersen (1994) ArticleTitleSpontaneous hybridization between oilseed rape (Brassica napus) and weedy B.campestris (Brassicaceae): a risk of growing genetically modified oilseed rape Am. J. Bot. 81 1620–1626

    Google Scholar 

  • M.S. Khan P. Maliga (1999) ArticleTitleFlourescent antibiotic resistance marker for tracking plastid transformation in higher plants Nat. Biotechnol. 17 910–914 Occurrence Handle10471936 Occurrence Handle1:CAS:528:DyaK1MXlslOmurw%3D

    PubMed  CAS  Google Scholar 

  • A.-S. Kiang V. Connolly D. McConnell T. Kavanagh (1994) ArticleTitlePaternal inheritance of mitochondria and chloroplasts in Festucapratensis-Loliumperenne intergeneric hybrids Theor. Appl. Genet. 87 681–688 Occurrence Handle10.1007/BF00222893

    Article  Google Scholar 

  • L. Landbo R.B. Jørgensen (1997) ArticleTitleSeed germination in weedy Brassica campestrisits hybrids with B. napus; Implications for risk assesment of oilseed rape Euphytica. 97 209–216 Occurrence Handle10.1023/A:1003032804340

    Article  Google Scholar 

  • G.Y. Lu G.S. Yang T.D. Fu (2004) ArticleTitleMolecular mapping of a dominant genic male sterility gene Ms in rapeseed (Brassica napus) Plant Breed. 123 262–265 Occurrence Handle1:CAS:528:DC%2BD2cXlvF2lt7c%3D

    CAS  Google Scholar 

  • T.R. Mikkelsen B. Andersen R.B. Jørgensen (1996) ArticleTitleThe risk of crop transgene spread Nature 380 31 Occurrence Handle10.1038/380031a0 Occurrence Handle1:CAS:528:DyaK28XhsFeis7w%3D

    Article  CAS  Google Scholar 

  • B.G. Milligan (1998) Total DNA isolation A.R. Hoelzel (Eds) Molecular Genetic Analysis of Populations: A Practical Approach IRL Press Oxford 29–64

    Google Scholar 

  • H.L. Mogensen (1996) ArticleTitleThe hows and whys of cytoplasmic inheritance in seed plants Am. J. Bot. 83 383–404

    Google Scholar 

  • M. Murgia S. Detchepare J.L. Vanwent M. Cresti (1991) ArticleTitle Brassica napus pollen development during generative cell and sperm cell-formation Sex. Plant Reprod. 4 176–181

    Google Scholar 

  • M. Pertl T.P. Hauser C. Damgaard R.B. Jørgensen (2002) ArticleTitleMale fitness of oilseed rape (Brassica napus), weedy B.rapatheir F1 hybrids when pollinating B. rapa seeds Heredity 89 212–218 Occurrence Handle10.1038/sj.hdy.6800131 Occurrence Handle12209392 Occurrence Handle1:CAS:528:DC%2BD38Xms1eqsL0%3D

    Article  PubMed  CAS  Google Scholar 

  • A.K. Pradhan S. Prakash A. Mukhopadhyay D. Pental (1992) ArticleTitlePhylogeny of Brassicaallied genera based on variation in chloroplast and mitochondrial DNA patterns – Molecular and taxonomic classifications are incongruous Theor. Appl. Genet. 85 331–340 Occurrence Handle10.1007/BF00222878

    Article  Google Scholar 

  • X. Reboud C. Zeyl (1994) ArticleTitleOrganelle inheritance in plants Heredity 72 132–140

    Google Scholar 

  • S. Ruf M. Hermann I.J. Berger H. Carrer R. Bock (2001) ArticleTitleStable genetic transformation of tomato plastids and expression of a foreign protein in fruit Nat. Biotechnol. 19 870–875 Occurrence Handle10.1038/nbt0901-870 Occurrence Handle11533648 Occurrence Handle1:CAS:528:DC%2BD3MXmvVajtLo%3D

    Article  PubMed  CAS  Google Scholar 

  • S. Scott M. Wilkinson (1999) ArticleTitleLow probability of chloroplast movement from oilseed rape (Brassica napus) into wild Brassica rapa Nat. Biotechnol. 17 390–393 Occurrence Handle10.1038/8623 Occurrence Handle10207890 Occurrence Handle1:CAS:528:DyaK1MXitl2gs74%3D

    Article  PubMed  CAS  Google Scholar 

  • V.A. Sidorov D. Kasten S.Z. Pang P.T.J. Hajdukiewicz J.M. Staub N.S. Nehra (1999) ArticleTitleStable chloroplast transformation in potato: use of green fluorescent protein as a plastid marker Plant J. 19 209–216 Occurrence Handle10.1046/j.1365-313X.1999.00508.x Occurrence Handle10476068 Occurrence Handle1:CAS:528:DyaK1MXmtlClsb8%3D

    Article  PubMed  CAS  Google Scholar 

  • S.R. Sikdar G. Serino S. Chaudhuri P. Maliga (1998) ArticleTitlePlastid transformation in Arabidopsis thaliana Plant Cell Rep. 18 20–24 Occurrence Handle10.1007/s002990050525 Occurrence Handle1:CAS:528:DyaK1MXhvF2htbw%3D

    Article  CAS  Google Scholar 

  • M. Skarjinskaia Z. Svab P. Maliga (2003) ArticleTitlePlastid transformation in Lesquerella fendlerian oilseed Brassicacea Transgenic Res. 12 115–122 Occurrence Handle10.1023/A:1022110402302 Occurrence Handle12650530 Occurrence Handle1:CAS:528:DC%2BD3sXnsFalsw%3D%3D

    Article  PubMed  CAS  Google Scholar 

  • A. Somanchi S.P. Mayfield (1999) ArticleTitleNuclear-chloroplast signalling Curr. Opin. Plant Biol. 2 404–409 Occurrence Handle10.1016/S1369-5266(99)00013-8 Occurrence Handle10508759 Occurrence Handle1:CAS:528:DyaK1MXmvFSis7k%3D

    Article  PubMed  CAS  Google Scholar 

  • C. Spillane M.D. Curtis U. Grossniklaus (2004) ArticleTitleApomixis technology development – virgin births in farmers′ fields? Nat. Biotechnol. 22 687–691 Occurrence Handle10.1038/nbt976 Occurrence Handle15175691 Occurrence Handle1:CAS:528:DC%2BD2cXksV2gt70%3D

    Article  PubMed  CAS  Google Scholar 

  • B.K. Staniland P.B.E. Mcvetty L.F. Friesen S. Yarrow G. Freyssinet M. Freyssinet (2000) ArticleTitleEffectiveness of border areas in confining the spread of transgenic Brassica napus pollen Can. J. Plant Sci. 80 521–526

    Google Scholar 

  • Z. Svab P.T.J. Hajdukiewicz P. Maliga (1990) ArticleTitleStable transformation of plastids in higher-plants Proc. Natl. Acad. Sci. USA 87 8526–8530 Occurrence Handle11607112 Occurrence Handle1:CAS:528:DyaK3MXhslWn

    PubMed  CAS  Google Scholar 

  • P. Taberlet L. Gielly G. Pautou J. Bouvet (1991) ArticleTitleUniversal primers for amplification of three non-coding regions of chloroplast DNA Plant Mol. Biol. 17 1105–1109 Occurrence Handle10.1007/BF00037152 Occurrence Handle1932684 Occurrence Handle1:CAS:528:DyaK38Xhslel

    Article  PubMed  CAS  Google Scholar 

  • Tolstrup K., Andersen S.B., Boelt B., Buus M., Gylling M. Holm P.B., Kjellson G., Pedersen S., Østergard H., Mikkel- sen S.A.Report from the Danish working group on the co- existence of genetically modified crops with conventional organic crops. 2003. Frederiksberg Bogtryk. DIAS report, Plant Production Ref Type: Report.

  • InstitutionalAuthorNameU.N. (1935) ArticleTitleGenome-analysis in Brassica with special reference to the experimental formation of B. napus and peculiar mode of fertilization Jpn. J. Bot. VII 389–453

    Google Scholar 

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Authors and Affiliations

  1. Plant Research Department, Risø National Laboratory, Roskilde, DK-4000, Denmark

    Marina M. Johannessen, Bente A. Andersen & Rikke B. Jørgensen

  2. Department of Terrestrial Ecology, National Environmental Research Institute, DK-8600, Silkeborg, Denmark

    Christian Damgaard

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  1. Marina M. Johannessen
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  2. Bente A. Andersen
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  3. Christian Damgaard
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  4. Rikke B. Jørgensen
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Correspondence to Rikke B. Jørgensen.

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Johannessen, M.M., Andersen, B.A., Damgaard, C. et al. Maternal Inheritance of Chloroplasts between Brassica rapa and F1-hybrids Demonstrated by cpDNA Markers Specific to Oilseed Rape and B. rapa. Mol Breeding 16, 271–278 (2005). https://doi.org/10.1007/s11032-005-0236-8

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  • DOI: https://doi.org/10.1007/s11032-005-0236-8

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