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Molecular diversity of rat brain proteins as revealed by proteomic analysis

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Summary

Multiple protein expression forms (MPEFs) presenting splicing forms or co- and posttranslation modifications, account for the vast diversity, the myriad of gene products and clearly indicate problems which proteomics research is facing. In the present study, we generated a rat brain map representing MPEFs by the use of an analytical method based on two-dimensional electrophoresis combined with mass spectrometry. Forty-nine individual proteins were selected that showed more than two spots, resulting altogether into a total number of 357 expression forms. Some proteins showed large MPEFs numbers as e.g. tubulin alpha-1 chain (24 spots), ATP synthase alpha-chain (28), beta chain (17) or septin 7 (13).

The molecular diversity observed in this map clearly shows that immunochemical or even protein chemical results from expressional studies have to be interpreted with caution, in particular if one dimensional electrophoretic or western blot techniques are applied and MPEFs are poorly resolved.

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Abbreviations

2D:

two-dimensional

2-DE:

two-dimensional gel electrophoresis

CaMK:

Ca2+/calmodulin-dependent protein kinase

DRP2:

dihydropyrimidinase related protein 2

DTT:

DL-dithiothreitol

MALDI-MS:

matrix-assisted laser desorption/ionization-mass spectrometry

MPEFs:

multiple protein expression forms

PKA:

protein kinase A

MAPK:

mitogen-activated protein kinase

PMSF:

phenylmethylsulfonyl fluoride

PTM:

posttranslational modifications

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Authors and Affiliations

  1. Department of Pediatrics, University of Vienna, Vienna, Austria

    Jae-Won Yang & Gert Lubec

  2. F. Hoffmann-La Roche, Genomics and Proteomics Technologies, Basel, Switzerland

    Jean-François Juranville & Michael Fountoulakis

  3. Institute for Animal Genetics, Himberg, Austria

    Harald Höger

  4. Center of Basic Research, Foundation for Biomedical Research of the Academy of Athens, Athens, Greece

    Michael Fountoulakis

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  1. Jae-Won Yang
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  2. Jean-François Juranville
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  3. Harald Höger
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  4. Michael Fountoulakis
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  5. Gert Lubec
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Correspondence to Gert Lubec.

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Yang, JW., Juranville, JF., Höger, H. et al. Molecular diversity of rat brain proteins as revealed by proteomic analysis. Mol Divers 9, 385–396 (2005). https://doi.org/10.1007/s11030-005-6359-3

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