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Metrological support for DNA sequencing

  • Medical and Biological Measurements
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Measurement Techniques Aims and scope

We discuss the operating principle of a pyrosequencer, which is an instrument for sequential reading of the nucleotide sequence of DNA molecules in pyrophosphate sequencing (pyrosequencing). We formulate the basic principles of metrological support for DNA sequencing and we present the results of studies of the metrological characteristics of the GS FLX pyrosequencer. We list the major problems encountered in designing a system for metrological support of DNA sequencing.

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References

  1. M. Margulies et al., “Genome sequencing in microfabricated high-density picolitre reactors,” Nature, No. 437, 376–380 (2005).

    Google Scholar 

  2. S. S. Golubev et al., “Selection of a reference standard for determination of DNA nucleotide sequences,” Izmer. Tekhn., No. 12, 45–47 (2011).

    Google Scholar 

  3. Federal Law of the Russian Federation No. 102-FZ, Assurance of the Uniformity in Measurement, Chap. 1, Sec. 2.

  4. International Vocabulary of Metrology. Basic and General Concepts and Associated Terms [Russian translation], Professional NPO, St. Petersburg (2010).

  5. V. A. Kuznetsov, L. K. Isaev, and I. A. Shaiko, Metrology [in Russian], STANDARTINFORM, Moscow (2005).

    Google Scholar 

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This work was done with the financial support of the Ministry of Education and Science of the Russian Federation (State Contract 16.648.11.3005 within the Federal Targrted Program on Infrastructure Development for the Nano Industry in the Russian Federation in 2008–2010).

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Authors and Affiliations

  1. All-Russia Research Institute of Metrological Service (VNIIMS), Moscow, Russia

    S. S. Golubev, S. A. Kononogov, Yu. A. Kudeyarov & P. Yu. Nikolaeva

  2. Bioengineering Center, Russian Academy of Sciences, Moscow, Russia

    A. V. Mardanov, N. V. Ravin & K. G. Skryabin

Authors
  1. S. S. Golubev
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  2. S. A. Kononogov
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  3. Yu. A. Kudeyarov
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  4. A. V. Mardanov
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  5. P. Yu. Nikolaeva
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  6. N. V. Ravin
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  7. K. G. Skryabin
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Corresponding author

Correspondence to S. A. Kononogov.

Additional information

Translated from Izmeritel’naya Tekhnika, No. 3, pp. 64–68, March, 2012.

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Golubev, S.S., Kononogov, S.A., Kudeyarov, Y.A. et al. Metrological support for DNA sequencing. Meas Tech 55, 345–350 (2012). https://doi.org/10.1007/s11018-012-9962-6

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  • DOI: https://doi.org/10.1007/s11018-012-9962-6

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