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Membrane-stabilizing and protective effects of curcumin in a rotenone-induced rat model of Parkinson disease

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Abstract

Parkinson disease (PD) is a chronic progressive neurodegenerative disease characterized by both motor and non-motor features. Numerous risk factors (oxidative stress, free radical formation, and several environmental toxins) have been associated with PD. The experimental studies were carried out under in vivo conditions. Biochemical data analysis indicated that compared with the parameters of control (C) rats, rotenone-induced PD rats showed a significant decrease in the specific content of the total fraction of isoforms of O2−-producing, heat-stable, NADPH-containing associates (NLP-Nox) from membrane formations of tissues (brain, liver, lung, and small intestine). Compared with the C group indices, in the PD and PD + curcumin (PD + CU) groups there is some change in the shape of the optical absorption spectra of isoforms associated with a change in the amount of Nox in the isoform composition of the total fraction of the NLP-Nox associate. Thus, daily administration of CU (200 mg/kg, i.p.) to PD rats for 63 days had a regulatory effect, bringing the specific content and O2−-producing activity of the total fraction of NLP-Nox isoforms closer to the norm. CU has membrane-stabilizing effects in rotenone-induced PD.

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Data availability

The data for this study are available from the corresponding author on reasonable request.

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Funding

The authors declare that no funds, grants, or other support were received during the preparation of this manuscript.

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Authors and Affiliations

  1. Sensorimotor Integration Lab, Orbeli Institute of Physiology NAS RA, 0028, Yerevan, Armenia

    L. V. Darbinyan, L. E. Hambardzumyan & L. P. Manukyan

  2. Neuroendocrine Relationships Lab, Orbeli Institute of Physiology NAS RA, 0028, Yerevan, Armenia

    K. V. Simonyan

  3. H. Buniatyan Institute of Biochemistry NAS RA, 0014, Yerevan, Armenia

    M. A. Simonyan & R. M. Simonyan

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  1. L. V. Darbinyan
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  6. L. P. Manukyan
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Contributions

All authors contributed to the study conception and design. LVD, MAS, RMS, KVS, LEH and LPM designed the study and conducted the experiment. KVS, LVD and MAS wrote the manuscript. All authors contributed to the revision final version of the manuscript.

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Correspondence to K. V. Simonyan.

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This study was performed in accordance with the principles of laboratory animal care of the Ethics Committee of Yerevan State Medical University (Yerevan, Armenia) and in accordance with the decision of 22 September 2010 of the Council of European Communities [2010/63/EU] and with “ARRIVE” guidelines (Animals in Research Reporting in vivo Experiments). All applicable international, national, and/or institutional guidelines for the care and use of animals were followed. All methods were carried out in accordance with relevant guidelines and regulations.

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Darbinyan, L.V., Simonyan, K.V., Hambardzumyan, L.E. et al. Membrane-stabilizing and protective effects of curcumin in a rotenone-induced rat model of Parkinson disease. Metab Brain Dis 38, 2457–2464 (2023). https://doi.org/10.1007/s11011-023-01237-z

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