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Circ_0000011 promotes cerebral ischemia/reperfusion injury via miR-27a-3p-dependent regulation of NRIP1

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A Correction to this article was published on 09 November 2022

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Abstract

Background

Cerebral ischemia/reperfusion (I/R) can result in brain function impairments. Circular RNAs (circRNAs) have emerged as vital regulators in cerebral I/R injury. However, the functions of mmu_circ_0000011 in cerebral I/R injury are still unclear. Thus, in this study, we aimed to explore the effect of mmu_circ_0000011 on cerebral I/R injury.

Methods

Oxygen-glucose deprivation and reperfusion (OGD/R)-induced HT-22 cells were used to mimic the condition of cerebral I/R injury in vitro. Cell Counting Kit-8 (CCK-8) assay, lactate dehydrogenase (LDH) assay, 5’-ethynyl-2’-deoxyuridine (EdU) assay and flow cytometry analysis were utilized to assess cell viability, LDH release, proliferation and apoptosis, respectively. qRT-PCR and western blot were performed to determined the levels of circ_0000011, miR-27a-3p and NRIP1. Dual-luciferase reporter assay and RNA pull-down assay were utilized to analyze the targeting relation of circ_0000011, miR-27a-3p and NRIP1.

Results

OGD/R treatment inhibited HT-22 cell viability and promoted LDH release, cell apoptosis and inflammation. Circ_0000011 level was increased in OGD/R-induced HT-22 cells. Silencing of circ_0000011 promoted cell proliferation and inhibited LDH release, apoptosis and inflammation in OGD/R-treated HT-22 cells. For mechanism analysis, circ_0000011 was demonstrated to sponge miR-27a-3p, which directly targeted NRIP1. MiR-27a-3p inhibition or NRIP1 overexpression ameliorated the impacts of circ_0000011 silencing on cell proliferation, LDH release, apoptosis and inflammation in OGD/R-treated HT-22 cells.

Conclusions

Circ_0000011 promotes OGD/R-induced HT-22 cell impairments by elevating NRIP1 through sponging miR-27a-3p.

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Authors and Affiliations

  1. Department of neurological function, the First Affiliated Hospital of Kangda College of Nanjing Medical University, Lianyungang, China

    Xiaolin Ren

  2. Department of Neurology, the First Affiliated Hospital of Kangda College of Nanjing Medical University, Lianyungang, China

    Xinyu Zhou

  3. Department of Neurology, the Affiliated Lianyungang Hospital of Xuzhou Medical University, No. 182, Tongguan North Road, Haizhou District, Lianyungang City, Jiangsu, China

    Xinyu Zhou

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  1. Xiaolin Ren
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Xiaolin Ren designed and performed the research; Xinyu Zhou analyzed the data; Xiaolin Ren wrote the manuscript. All authors read and approved the final manuscript.

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Correspondence to Xinyu Zhou.

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Ren, X., Zhou, X. Circ_0000011 promotes cerebral ischemia/reperfusion injury via miR-27a-3p-dependent regulation of NRIP1. Metab Brain Dis 38, 295–306 (2023). https://doi.org/10.1007/s11011-022-00972-z

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