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Circular RNA circSLC8A1 inhibits the proliferation and invasion of glioma cells through targeting the miR-214-5p/CDC27 axis

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Abstract

Circular RNA circSLC8A1 is one of the cancer-related circRNAs that is implicated in various cancers. However, studies focusing on the role of circSLC8A1 in glioma is rare. Here we attempted to evaluate the biological function of circSLC8A1 in glioma and explore the potential mechanism. The relative expression of circSLC8A1, miR-214-5p and CDC27 in tissues and cell lines was determined by qRT-PCR. Cell proliferation and invasion were respectively measured by CCK-8 and transwell assays. Protein level of CDC27 was analyzed by western blot. Luciferase reporter assay was performed to confirm the regulatory interaction of cirRNA-miRNA-mRNA. Lowly expressed circSLC8A1 was observed in both glioma tissues and cell lines. Further biological analyses showed that circSLC8A1 inhibits the cell proliferation and invasion of glioma cells. CircSLC8A1 directly sponged miR-214-5p and inhibited miR-214-5p expression in glioma cells. CDC27 was a direct target of miR-214-5p and could be regulated by miR-214-5p. Moreover, miR-214-5p mimics and CDC27 knockdown reversed the inhibitory effects of circSLC8A1 on cell proliferation and invasion. Taken together, our results demonstrated a tumor suppressive role of circSLC8A1 in glioma through regulation of glioma cells proliferation and invasion. The effects of circSLC8A1 were mediated by miR-214-5p/CDC27 axis. Our study provided a new understanding of the occurrence and development of glioma.

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Data availability

The data that support the findings of this study are available from the corresponding author.

upon reasonable request.

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Authors and Affiliations

  1. Department of Neurosurgery, Yuncheng Central Hospital of Shanxi Medical University, Yuncheng, 044000, China

    Jiabao Zhu, Haizheng Cui, Baochen Zhang & Guoqiang Xue

  2. Department of Neurosurgery, Shaanxi Provincial People’s Hospital, Xi’an, 710068, China

    Xiaobin Liu

  3. Department of Pathology, Yuncheng Central Hospital of Shanxi Medical University, Yuncheng, 044000, China

    Zhonghua Luan

  4. Department of Radiology, Yuncheng Central Hospital of Shanxi Medical University, Yuncheng, 044000, China

    Wei Xue

Authors
  1. Jiabao Zhu
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  2. Xiaobin Liu
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  3. Zhonghua Luan
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  4. Wei Xue
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  5. Haizheng Cui
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  6. Baochen Zhang
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  7. Guoqiang Xue
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Contributions

Jiabao Zhu and Xiaobin Liu designed the study, conducted the experiments and drafted the manuscript. Haizheng Cui and Baochen Zhang collected and analyzed the data. Zhonghua Luan and Wei Xue contributed the methodology and analyzed the data. Guoqiang Xue operated wrote the manuscript.

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Correspondence to Jiabao Zhu.

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These studies were approved by Hospital Ethic Review Committee (APPROVAL NO. KYLL2019032).

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Zhu, J., Liu, X., Luan, Z. et al. Circular RNA circSLC8A1 inhibits the proliferation and invasion of glioma cells through targeting the miR-214-5p/CDC27 axis. Metab Brain Dis 37, 1015–1023 (2022). https://doi.org/10.1007/s11011-022-00915-8

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  • DOI: https://doi.org/10.1007/s11011-022-00915-8

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