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EIF4A3-induced circCCNB1 (hsa_circ_0001495) promotes glioma progression by elevating CCND1 through interacting miR-516b-5p and HuR

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Abstract

To explore the functions of circRNA cyclin B1 (circCCNB1) in glioma and its possible mechanisms. The expression of circCCNB1, eukaryotic translation initiation factor 4A3 (EIF4A3), cyclin D1 (CCND1) and miR-516b-5p was determined by qRT-PCR, western blot or immunohistochemistry (IHC) assay. The feature of circCCNB1 was analyzed by Actinomycin D (ActD), RNase R and subcellular fraction assays. The molecule relationships were analyzed by RIP, dual-luciferase reporter and RNA pull-down assays. CCK-8, EdU and colony formation assays were performed to analyze cell proliferation. Flow cytometry analysis was executed to estimate the cell cycle. Murine xenograft model assay was used for the role of circCCNB1 in vivo. CircCCNB1 was overexpressed in glioma tissues and cells. EIF4A3 positively regulated circCCNB1 expression. CircCCNB1 knockdown repressed glioma cell proliferation and cell cycle process in vitro and blocked tumor growth in vivo. CircCCNB1 knockdown reduced CCND1 expression in glioma cells and CCND1 overexpression bated the effect of circCCNB1 knockdown on glioma cell growth. CircCCNB1 interacted with HuR to elevate CCND1 expression. miR-516b-5p could interact with circCCNB1 and CCND1. CircCCNB1 regulated glioma cell progression and CCND1 expression by miR-516b-5p and HuR. CircCCNB1 aggravated glioma cell growth by elevating CCND1 through targeting miR-516b-5p and HuR.

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Author notes

  1. Xiaoli Li and Chengmou Wang contributed equally to this work.

Authors and Affiliations

  1. Department of Neurology, Renmin Hospital, Hubei University of Medicine, No. 39, Chaoyang Middle Road, Maojian District, Shiyan, 442000, China

    Xiaoli Li, Chengmou Wang, Guanghui Chen, Wenqin Zou, Yanqing Deng & Faming Zhou

Authors
  1. Xiaoli Li
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  2. Chengmou Wang
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  3. Guanghui Chen
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  4. Wenqin Zou
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  5. Yanqing Deng
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  6. Faming Zhou
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Contributions

Faming Zhou designed and supervised the study. Xiaoli Li and Chengmou Wang conducted the experiments and drafted the manuscript. Guanghui Chen and Wenqin Zou collected and analyzed the data. Yanqing Deng contributed the methodology, operated the software and edited the manuscript.

Corresponding author

Correspondence to Faming Zhou.

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The authors declare that they have no conflicts of interest.

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The project was authorized by the Ethics Committee of Renmin Hospital, Hubei University of Medicine. All participants provided written informed consents.

The Ethics Committee of Animal Research of Renmin Hospital, Hubei University of Medicine approved the animal study.

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Li, X., Wang, C., Chen, G. et al. EIF4A3-induced circCCNB1 (hsa_circ_0001495) promotes glioma progression by elevating CCND1 through interacting miR-516b-5p and HuR. Metab Brain Dis 37, 819–833 (2022). https://doi.org/10.1007/s11011-021-00899-x

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  • DOI: https://doi.org/10.1007/s11011-021-00899-x

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