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Downregulation of lncRNA MEG3 is involved in Parkinson’s disease

Abstract

Background

Long non-coding RNA (lncRNA) MEG3 regulates human cancers, while its role in Parkinson’s disease (PD) is unknown. The present study explored the involvement of MEG3 in PD.

Methods

This study enrolled PD patients (n = 79) and healthy controls (n = 62) who were admitted to the Second Affiliated Hospital of Nanchang University from May 2016 to March 2018. RT-qPCR was performed to measure the expression of MEG3 and LRRK2. ROC curve analysis was performed for diagnostic analysis. Cell transfections were performed to analyze the interaction between MEG3 and LRRK2. Cell apoptosis and MTT assays were performed to evaluate the effect of cell transfections on cell apoptosis and viability.

Results

MEG3 was downregulated in PD patients compared to that in the healthy controls. ROC curve analysis showed altered expression of MEG3 in PD patients. MEG3 was also down-regulated in SH-SY5Y cells treated with MPP + . Overexpression of MEG3 increased the expression levels of leucine-rich repeat kinase 2 (LRRK2) in SH-SY5Y cells. In contrast, overexpression of LRRK2 showed no significant effects on MEG3. Overexpression of MEG3 improved the viability and inhibited the apoptosis of SH-SY5Y cells pretreated with MPP + .

Conclusions

In conclusion, lncRNA MEG3 is downregulated in PD and may affect the expression of LRRK2 to regulate cell viability and apoptosis involved in PD.

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Data Availability

The data that support the findings of this study are available on request from the corresponding author.

The data are not publicly available due to their containing information that could compromise the privacy of research participants.

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Funding

This research did not receive any specific grant from funding agencies in the public, commercial, or not-for-profit sectors.

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Authors

Contributions

Hui Huang, Suyue Zheng: study concepts, literature research, clinical studies, data analysis, experimental studies, manuscript writing and review; Mingwei Lu: study design, literature research, experimental studies and manuscript editing; Hui Huang, Suyue Zheng: definition of intellectual content, clinical studies, data acquisition and statistical analysis; Hui Huang: data acquisition, manuscript preparation and data analysis.

Corresponding author

Correspondence to Mingwei Lu.

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Ethical approval and consent to participate

All patients signed the written informed consent. All producers were approved by the Second Affiliated Hospital of Nanchang University Ethics Committee. Procedures operated in this research were completed in keeping with the standards set out in the Announcement of Helsinki and laboratory guidelines of research in China.

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Not applicable.

All authors have read and approve the submission of the manuscript.

Competing interest

All other authors have no conflict of interest.

We declare that we do not have any commercial or associative interest that represents a conflict of interest in connection with the work submitted.

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Hui Huang and Suyue Zheng are co-first authors

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Cite this article

Huang, H., Zheng, S. & Lu, M. Downregulation of lncRNA MEG3 is involved in Parkinson’s disease. Metab Brain Dis 36, 2323–2328 (2021). https://doi.org/10.1007/s11011-021-00835-z

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Keywords

  • Parkinson’s disease
  • lncRNA MEG3
  • Leucine-rich repeat kinase 2