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miR-219 overexpressing oligodendrocyte progenitor cells for treating compression spinal cord injury

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Abstract

Oligodendrocyte progenitor cells (OPCs) transplantation has been considered a promising treatment for spinal cord injury, according to previous studies. Recent research shed light on the importance of microRNA 219 (miR-219) in oligodendrocyte development, so here miR-219-overexpressing OPCs (miR-219 OPCs) were transplanted in animal models of spinal cord injury to evaluate the impact of miR-219 on oligodendrocyte differentiation and functional recovery in vivo. Our findings demonstrate that transplanted cells were distributed in the tissue sections and contributed to reducing the size of cavity in the injury site. Interestingly, miR-219 promoted OPC differentiation into mature oligodendrocyte expressing MBP in vivo whereas in absence of miR-219, less number of cells differentiated into mature oligodendrocytes. An eight week evaluation using the Basso Beattie Bresnahan (BBB) locomotor test confirmed improvement in functional recovery of hind limbs. Overall, this study demonstrated that miR-219 promoted differentiation and maturation of OPCs after transplantation and can be used in cell therapy of spinal cord injury.

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All data generated or analyzed during this study are included in this published article.

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Funding

This study was funded by Tehran University of Medical Sciences (grant number 93-04-87-27710).

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Authors and Affiliations

  1. Department of Medical Biotechnology, School of Advanced Technologies in Medicine, Tehran University of Medical Sciences, Tehran, Iran

    Bahareh Nazari & Esmaeil Sadroddiny

  2. Department of Anatomical Sciences, School of Medicine, Ardabil University of Medical Sciences, Ardabil, Iran

    Zeinab Namjoo

  3. Department of Anatomy, School of Medicine, Iran University of Medical Sciences, Tehran, Iran

    Fatemeh Moradi

  4. Department of Applied Cell Sciences, Faculty of Medicine, Kashan University of Medical Sciences, Kashan, Iran

    Mansure Kazemi

  5. Department of Tissue Engineering and Applied Cell Sciences, School of Advanced Technologies in Medicine, Tehran University of Medical Sciences, Tehran, Iran

    Somayeh Ebrahimi-Barough & Jafar Ai

Authors
  1. Bahareh Nazari
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  2. Zeinab Namjoo
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  3. Fatemeh Moradi
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  4. Mansure Kazemi
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  5. Somayeh Ebrahimi-Barough
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  6. Esmaeil Sadroddiny
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  7. Jafar Ai
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Contributions

Jafar Ai, Esmaeil Sadroddiny, and Bahareh Nazari designed the study. Bahareh Nazari, Zeinab Namjoo, and Fateme Moradi performed the practical parts of the study. Bahareh Nazari, Mansure Kazemi, and Somayeh Ebrahimi-Barough contribute to writing the manuscript. Esmaeil Sadroddiny, and Jafar Ai supervised the study. All authors read and approved the final manuscript.

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Correspondence to Esmaeil Sadroddiny or Jafar Ai.

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All applicable international, national, and/or institutional guidelines for the care and use of animals were followed.

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Nazari, B., Namjoo, Z., Moradi, F. et al. miR-219 overexpressing oligodendrocyte progenitor cells for treating compression spinal cord injury. Metab Brain Dis 36, 1069–1077 (2021). https://doi.org/10.1007/s11011-021-00701-y

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  • DOI: https://doi.org/10.1007/s11011-021-00701-y

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