Abstract
The blood-brain barrier (BBB) comprises the interface between blood, brain and cerebrospinal fluid. Its primary function, which is mainly carried out by tight junctions, is to stabilize the tightly controlled microenvironment of the brain. To study the development and maintenance of the BBB, as well as various roles their intrinsic mechanisms that play in neurological disorders, suitable measurements are required to demonstrate integrity and functional changes at the interfaces between the blood and brain tissue. Markers and plasma proteins with different molecular weight (MW) are used to measure the permeability of BBB. In addition, the expression changes of tight-junction proteins form the basic structure of BBB, and imaging modalities are available to study the disruption of BBB. In the present review, above mentioned methods are depicted in details, together with the pros and cons as well as the differences between these methods, which maybe benefit research studies focused on the detection of BBB breakdown.
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This work was supported by National Natural Science Foundation of China (82000803), Guangdong Basic and Applied Basic Research Foundation (2019A1515010980), the Fundamental Research Funds for the Central Universities (20ykpy96).
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Nannan Sun, Chaohui Duan had the idea for the article. Huixin Sun, Huiling Hu had drafted and critically revised the manuscript. All the authors approved final version of manuscript.
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Sun, H., Hu, H., Liu, C. et al. Methods used for the measurement of blood-brain barrier integrity. Metab Brain Dis 36, 723–735 (2021). https://doi.org/10.1007/s11011-021-00694-8
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DOI: https://doi.org/10.1007/s11011-021-00694-8