Abstract
D-galactose (D-gal) is a carbohydrate widely distributed in regular diets. However, D-gal administration in rodents is associated with behavioral and neurochemical alterations similar to features observed in aging. In this regard, this study aimed to investigate the effects of D-gal exposure, in different periods, in rats’ brain regions’ activities of creatine kinase (CK) and tricarboxylic acid (TCA) cycle enzymes. Male adult Wistar rats received D-gal (100 mg/kg, gavage) for 1, 2, 4, 6 or 8 weeks. CK and TCA enzymes’ activities were evaluated in rats’ prefrontal cortex and hippocampus. In general, the results showed an increase in citrate synthase (CS) and succinate dehydrogenase (SDH) activities in animals treated with D-gal compared to the control group in the prefrontal cortex and hippocampus. Also, in the fourth week, the malate dehydrogenase (MD) activity increased in the hippocampus of rats that received D-gal compared to control rats. In addition, we observed an increase in the CK activity in the prefrontal cortex and hippocampus in the first and eighth weeks of treatment in the D-gal group compared to the control group. D-gal administration orally administered modulated TCA cycle enzymes and CK activities in the prefrontal cortex and hippocampus, which were also observed in aging and neurodegenerative diseases. However, more studies using experimental models are necessary to understand better the impact and contribution of these brain metabolic abnormalities associated with D-gal consumption for aging.
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Experimental Neurology Laboratory (Brazil) is funded by grants from Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq), Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES), Fundação de Amparo à Pesquisa e Inovação do Estado de Santa Catarina (FAPESC) and UNESC. JB is 2 CNPq Research Fellow. ELS and JQ are 1 CNPq Research Fellow.
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Study design: Josiane Budni; Michelle Lima Garcez; Emílio Luiz Streck; João Quevedo. Study conduct and data collection: Arleide Braga Brandão; Sabrina da Silva; Michelle Lima Garcez; Francielle Mina; Tatiani Bellettini-Santos; Gabriel Casagrande Zabot; Eduarda Behenck Medeiros; Giselli Scaini. Data analysis: Giselli Scaini; Michelle Lima Garcez; Jade de Oliveira. Drafting manuscript: Josiane Budni; Michelle Lima Garcez; Jade de Oliveira; Emílio Luiz Streck; João Quevedo. Wrote the manuscript: Josiane Budni; Michelle Lima Garcez; Jade de Oliveira. Approving final version of manuscript: all the authors.
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Budni, J., Braga Brandão, A., da Silva, S. et al. Oral administration of D-galactose increases brain tricarboxylic acid cycle enzymes activities in Wistar rats. Metab Brain Dis 36, 1057–1067 (2021). https://doi.org/10.1007/s11011-021-00682-y
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DOI: https://doi.org/10.1007/s11011-021-00682-y