Metabolic Brain Disease

, Volume 33, Issue 3, pp 823–827 | Cite as

Hibiscus rosa sinensis mediate anxiolytic effect via modulation of ionotropic GABA-A receptors: possible mechanism of action

  • Zubia Begum
  • Ishrat Younus
Original Article


The current study was designed with the aim to investigate anti-anxiety potential of Hibiscus rosa sinensis roots and its possible mechanism of action. For this purpose hole board test, elevated plus maze test and light/dark exploration test were employed. The ethanol extract of plant was administered orally at two different doses i.e. 100 and 500 mg/kg for consecutive 14 days. The results of present investigation indicate that plant extract significantly (p < 0.05) increased the number of head dips and rearings as compared to control on respective days of observation. The extract increased the time of permanence in open arms and the number of head dips in elevated plus maze. In light/dark test, our study indicate that Hibiscus rosa sinensis significantly (p < 0.05) increased the time spent in light compartment and number of entries as compared to control. In addition the anxiolytic effects of HRS at highest tested dose were blocked by flumazenil, a GABA-A receptor antagonist that indicate that Hibiscus rosa sinensis potentiated the GABAergic actions. The results propose that the ethanol extract of Hibiscus rosa sinensis has prospective anxiolytic effect in mice via inhibition of ionotropic GABA receptors, using different behavioral paradigms.


Hibiscus rosa sinensis Ethanol extract Mice Anxiolytic Flumazenil Diazepam 



Authors would like to acknowledge Hamdard University Research Committee (HURC) for providing research grant to conduct this piece of work.

Compliance with ethical standards

Conflict of interest


Ethical approval

The ethical clearance of present study was approved by Ethical Review Board (ERB) of Hamdard University (No. ERB-16-01, dated 29–04-2016). Specifications described in Helsinki Resolution 1964 were adopted for animal handling in this research.


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© Springer Science+Business Media, LLC, part of Springer Nature 2018

Authors and Affiliations

  1. 1.Department of Pharmacology, Faculty of PharmacyHamdard UniversityKarachiPakistan

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