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Metabolic Brain Disease

, Volume 32, Issue 3, pp 743–749 | Cite as

Phytochemical screening, acute toxicity, anxiolytic and antidepressant activities of the Nelumbo nucifera fruit

  • Muhammad Ali Rajput
  • Rafeeq Alam KhanEmail author
Original Article

Abstract

Recently use of herbal therapies and diet rich in flavonoids and vitamin C have increased significantly to treat minor to modest anxiety disorders and various forms of depression. But further research and studies are necessary to evaluate the pharmacological & toxicological effects of plants. Hence present study was designed to conduct phytochemical screening, acute toxicity study, anxiolytic and antidepressant activities of the ethanol extract of Nelumbo nucifera fruit in order to ascertain its therapeutic potential. The qualitative phytochemical screening of the seed pods of the N. nucifera fruit extract exposed the existence of flavonoids, saponins, alkaloids, tannins and terpenoids in it. The acute toxicity of the N. nucifera fruit extract in mice revealed its LD50 value to be greater than 5000 mg/kg. Antianxiety activity was determined by elevated plus maze and light and dark test using 35 male Wister rats weighing 200–220 g which were equally divided in to 5 groups. The animals used in EPM underwent testing in light and dark box just 30 min after EPM. The antidepressant effect was assessed by forced swimming test using 35 male albino mice weighing 20–25 g equally divided in to 5 groups. In elevated plus maze, N. nucifera fruit extract exhibited substantial rise in number of open arm entries and time spent in open arms at dose 50 mg/kg while highly noteworthy increase in both parameters were observed at extract doses 100 and 200 mg/kg as compared to control. In light dark test highly significant increase in the percentage of time spent in light compartment was observed as compared to control. In forced swimming test highly noteworthy decline in duration of immobility was recorded at doses 100 and 200 mg/kg on 15th day i-e after administration of 14 doses, as compared to control; whereas same doses demonstrated significant decrease as compared to control in duration of immobility after single dose administration i-e on 2nd day of experiment. Thus N. nucifera fruit have exhibited strong anxiolytic and antidepressant effects and proved to have a great potential for therapeutic applications such as anxiety and depression and thus encourage more preclinical and clinical trials in this field.

Keywords

Nelumbo nucifera Anxiolytic Elevated plus maze Light and dark test 

Notes

Acknowledgements

The authors are thankful to Dr. Mohtesheem, Associate Professor and Chairman Department of Pharmacognosy for identification and authentication of N. nucifera fruits. The authors are also thankful to Prof. Dr. Iqbal Chaudary, Director, HEJ, Research Institute of Chemistry for granting permission to use Institute laboratory and workshop facilities.

References

  1. Ahmed S, Naved A, Khan RA, Siddiqui S (2015) Analgesic activities of methanol extract of Terminalia chebula fruit. Pharmacology & Pharmacy 6:547–553. doi: 10.4236/pp.2015.612056 CrossRefGoogle Scholar
  2. Edeoga HO, Okwu DE, Mbaebie BO (2005) Phytochemical constituents of some Nigerian medicinal plants. Afr J Biotechnol 4:685–688CrossRefGoogle Scholar
  3. Gong ZH, Li YF, Zhao N, Yang HJ, Su RB, Luo ZP, Li J (2006) Anxiolytic effect of agmatine in rats and mice. Eur J Pharmacol 550:112–116CrossRefPubMedGoogle Scholar
  4. Hossain MS, Ahmed M, Islam A (2010) Hypolipidemic and hepatoprotective effects of different fractions of ethanolic extract of immature leaves of Mangifera indica (Linn.) in alloxan induced diabetic rats. IJPSR 1:132–138Google Scholar
  5. Kessler A, Sahin-Nadeem H, Lummis SC, Weigel I, Pischetsrieder M, Buettner A, Villmann C (2014) GABAA receptor modulation by terpenoids from Sideritis extracts. Mol Nutr Food Res 58:851–862CrossRefPubMedGoogle Scholar
  6. Kumar NG, Sanjay BK (2005) Involvement of GABA in nootropic and anxiolytic activity of saponins of Albizzia Lebbeck leaves. Acta Hortic 675:115–121Google Scholar
  7. Kumar S, Sharma A (2005) Anti-anxiety activity studies of various extracts of Turnera aphrodisiaca ward. J Herb Pharmacother 5:13–21CrossRefPubMedGoogle Scholar
  8. Lorke D (1983) A new approach to practical acute toxicity testing. Arch Toxicol 54:275–287CrossRefPubMedGoogle Scholar
  9. Mansouri MT, SoltaniM NB, Farbood Y, Mashak A, Sarkaki A (2014) A possible mechanism for the anxiolytic-like effect of gallic acid in the rat elevated plus maze. Pharmacol Biochem Behav 117:40–46. doi: 10.1016/j.pbb.2013.12.011 CrossRefPubMedGoogle Scholar
  10. Michel B, Martine H (2003) The mouse light/dark box test. Eur J Pharmacol 463:55–65CrossRefGoogle Scholar
  11. Mukherjee PK, Mukherjee D, Maji AK, Rai S, Mukherjee PK, Saha K, Pal M, Saha BP, Heinrich M (2009) The sacred lotus (Nelumbo nucifera) – phytochemical and therapeutic profile. J Pharm Pharmacol 61:407–422CrossRefPubMedGoogle Scholar
  12. National Advisory Committee for Laboratory Animal Research (2004) Guidelines on the care and use of animals for scientific purposes. P 24Google Scholar
  13. National Institute of Health Guidelines for Care and Use of Laboratory Animals in Biomedical Research (2010) Guide for the care and use of laboratory animals. Prepublication draft. 8th edition. The National Academies Press Washington, D.C: 6, 47Google Scholar
  14. O’Donovan A, Slavich GM, Epel ES, Neylan TC (2013) Exaggerated neurobiological sensitivity to threat as a mechanism linking anxiety with increased risk for diseases of aging. Neurosci Biobehav Rev 37:96–108CrossRefPubMedGoogle Scholar
  15. Paudel KR, Panth N (2015) Phytochemical profile and biological activity of Nelumbo nucifera. Evid Based Complement Alternat Med 2015:789124. doi: 10.1155/ 2015/789124 CrossRefPubMedPubMedCentralGoogle Scholar
  16. Porsolt RD, Le Pichon M, Jalfre M (1977) Depression: a new animal model sensitive to antidepressant treatments. Nature 266:730–732CrossRefPubMedGoogle Scholar
  17. Rajput MA, Khan RA, Qazi N, Feroz Z (2012) Effect of methanol extract of ajwain (Trachyspermum Ammi L) on blood coagulation in rats. JLUMHS 11:105–108Google Scholar
  18. Rajput MA, Khan RA, Feroz Z (2013) Evaluation of antiepileptic activity of methanol extract of Trachyspermum Ammi. L. Arch Biol Sci Belgrade 65:815–819CrossRefGoogle Scholar
  19. Rajput MA, Khan RA, Feroz Z (2015) Evaluation of anxiolytic activity of methanol extract of Trachyspermum Ammi. L. Int J Pharm Pharm Sci 7:69–71Google Scholar
  20. Rauniar GP, Deo S, Bhattacharya SK (2007) Evaluation of anxiolytic activity of tensarin in mice. Kathmandu Univ Med J 18:188–194Google Scholar
  21. Reddy CM, Gawade SP (2006) Evaluation of the effects of photooxidized Echis carinatus venom on learning, memory and stress. J. Venom. Anim. Toxins incl. Trop. Dis 12(4) Botucatu. doi: 10.1590/S1678–91992006000400010
  22. Riaz A, Khan RA (2014) Effect of Punica granatum on behavior in rats. Afr J Pharm Pharmacol 8:1118–1126Google Scholar
  23. Sofowora EA (1993) Phytochemical assays. In: Medicinal plants and traditional medicine in Africa, 3rd edn. Spectrum Books Ltd, Nigeria, pp 150–153Google Scholar
  24. Sridhar KR, Bhat R (2007) Lotus: a potential nutraceutical source. J Agri Technol 3:143–155Google Scholar
  25. Sugimoto Y, Furutani S, Itoh A, Tanahashi T, Nakajima H, Oshiro H, Sun S, Yamada J (2008) Effects of extracts and neferine from the embryo of Nelumbo nucifera seeds on the central nervous system. Phymed 15(12):1117–1124Google Scholar
  26. Sugimoto Y, Furutani S, Nishimura K, Itoh A, Tanahashi T, Nakajima H, Oshiro H, Sun S, Yamada J (2010) Antidepressant-like effects of neferine in the forced swimming test involve the serotonin1A (5-HT1A) receptor in mice. Eur J Pharmacol 634:62–67CrossRefPubMedGoogle Scholar
  27. Sugimoto Y, Nishimura K, Itoh A, Tanahashi T, Nakajima H, Oshiro H, Sun S, Toda T, Yamada J (2015) Serotonergic mechanisms are involved in antidepressant-like effects of bisbenzylisoquinolines liensinine and its analogs isolated from the embryo of Nelumbo nucifera Gaertner seeds in mice. J Pharm Pharmacol 67:1716–1722CrossRefPubMedGoogle Scholar
  28. Taiwo AE, Leite FB, Lucena GM, Barros M, Silveira D, Silva MV, Ferreira VM (2012) Anxiolytic and antidepressant-like effects of Melissa officinalis (lemon balm) extract in rats: influence of administration and gender. Indian J Pharmacol 44:189–192CrossRefPubMedPubMedCentralGoogle Scholar
  29. Walf AA, Frye CA (2007) The use of the elevated plus maze as an assay of anxiety-related behavior in rodents. Nat Protoc 2:322–328CrossRefPubMedPubMedCentralGoogle Scholar
  30. Walpole RE (1982) Introduction of statistics. Third edition, Macmillan publishing company, Inc. N Y 1982:247–304Google Scholar
  31. Wei XY, Yang JY, Wang JH, Wu CF (2007) Anxiolytic effect of saponins from panax quinoque folium in mice. J Ethnopharmacol 111(3):613–618CrossRefPubMedGoogle Scholar
  32. William ZP, Leo EH (2007) Antidepressant agents, Katzung ed 10th; 2007, pp 475–488Google Scholar
  33. Wilner P (1984) The validity of animal models of depression. Psychopharmacology 83:1–16CrossRefGoogle Scholar
  34. Yoshimura H, Yamakawa K (2000) Animal models for behavioral disorder in females. Brain Sci 22:49–54Google Scholar

Copyright information

© Springer Science+Business Media New York 2017

Authors and Affiliations

  1. 1.Department of Pharmacology, Faculty of Pharmacy and Pharmaceutical SciencesUniversity of KarachiKarachiPakistan

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