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Total glycosides of Paeony shows Neuroprotective effects against Semen Strychni-induced neurotoxicity by recovering secretion of hormones and improving brain energy metabolism

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Abstract

In this study, we investigated the protective effect of total glycosides of paeony against Semen Strychni-induced neurotoxicity and discussed some probably mechanisms. Levels of estrone, estradiol, estriol and growth hormone in male rats’ serum were determined by ELISA, levels of ATP and substances associated with energy metabolism in rats’ brain were determined by HPLC and levels of progesterone was determined by a UPLC-MS/MS method. The results showed that neurotoxicity induced by Semen Strychni could cause a significant decrease (p < 0.05, compare to the blank group) in secretion of estrogens and GH and disorder brain energy metabolism at the same time. While, rats with total glycosides of paeony pre-protection (orally administrated with total glycosides of paeony for 15 days before administrating Semen Strychni extract) showed a much better condition in the secretion of hormones and brain energy metabolism, and showed no significant changes in most of those associated substances when comparing to the blank group. Our study indicated that total glycosides of paeony have neuroprotective effects on Semen Strychni-induced neurotoxicity. It could recover the disordered hormone secretion and improve the brain energy metabolism. Total glycosides of paeony is potential to be further used in clinic to protect against neurotoxicity induced by other reasons.

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Abbreviations

TBI:

traumatic brain injury

SAH:

subarachnoid hemorrhage

HIE:

hypoxic ischemic encephalopathy

GHD:

Growth Hormone Deficiency

ELISA:

Enzyme Linked Immunosorbent Assay

HPLC:

High Performance Liquid Chromatography

UPLC-MS/MS:

Ultra-performance Liquid Chromatography Tandem Mass-spectrometry

P4:

progesterone

MPA:

medroxyprogesterone acetate

E1:

estrone

E2:

estradiol

E3:

estriol

GH:

growth hormone

ATP:

adenosine triphosphate

ADP:

adenosine diphosphate

AMP:

adenosine monophosphate

E.C.:

Energy Charge

TGP:

total glycosides of paeony

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Acknowledgements

This research was funded by the National Natural Science Foundation of China No.81673577.

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Authors and Affiliations

  1. School of Pharmacy, Shenyang Pharmaceutical University, Shenyang, 110016, China

    Chenzhi Hou & Xiaohui Chen

  2. Southern Research Institute, 2000 9th Ave.s., Birmingham, AL, 35205, USA

    Ruowen Zhang

  3. School of Traditional Chinese Materia Medica, Shenyang Pharmaceutical University, Shenyang, 110016, China

    Kexia Zhang

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  1. Chenzhi Hou
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  2. Ruowen Zhang
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  4. Xiaohui Chen
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Correspondence to Xiaohui Chen.

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Hou, C., Zhang, R., Zhang, K. et al. Total glycosides of Paeony shows Neuroprotective effects against Semen Strychni-induced neurotoxicity by recovering secretion of hormones and improving brain energy metabolism. Metab Brain Dis 32, 2033–2044 (2017). https://doi.org/10.1007/s11011-017-0082-5

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