Acute psychoactive and toxic effects of D. metel on mice explained by 1H NMR based metabolomics approach
- 188 Downloads
Datura metel L. (D. metel) is one well-known folk medical herb with wide application and also the most abused plants all over the world, mainly for spiritual or religious purpose, over-dosing of which often produces poisonous effects. In this study, mice were orally administered with the extract of D. metel once a day at doses for 10 mg/kg and 40 mg/kg for consecutive 4 days, 1H NMR based metabolomics approach aided with histopathological inspection and biochemical assays were used for the first time to study the psychoactive and toxic effects of D. metel. Histopathological inspection revealed obvious hypertrophy of hepatocytes, karyolysis and karyorrhexis in livers as well as distinct nerve cell edema, chromatolysis and lower nuclear density in brains. The increased tissue level of methane dicarboxylic aldehyde (MDA) and superoxide dismutase (SOD), decreased tissue level of glutathione (GSH) along with increased serum level of aspartate aminotransferase (AST) and alanine aminotransferase (ALT) suggested brain and liver injury induced by D. metel. Orthogonal signal correction–partial least squares–discriminant analysis (OSC–PLS–DA) of NMR profiles supplemented with correlation network analysis revealed significant altered metabolites and related pathway that contributed to oxidative stress, energy metabolism disturbances, neurotransmitter imbalance and amino acid metabolism disorders.
KeywordsD. metel Mice Toxicity NMR Metabolomics
Compliance with ethical standards
This work was supported by the National Natural Science Foundation of China (No.81173526) and the Fundamental Research Funds for the Central Universities (No. 30916011307). We are grateful to Prof. Lan Yi for 1H NMR technical assistance.
Conflict of interest
The authors declare that they have no conflict of interest.
- Devi MR, Bawari M, Paul S, Sharma G (2011) Neurotoxic and medicinal properties of Datura stramonium L.–review Assam University. J Sci Technol 7:139–144Google Scholar
- DEVI MR, BAWARI M, Paul S, Sharma G (2012) Characterization of the toxic effects induced by Datura stramonium L. leaves on mice: a behavioral, biochemical and ultrastructural approach. Asian J Pharm Clin Res 5:143–146Google Scholar
- Etibor TA, Ajibola MI, Buhari MO, Safiriyu AA, Akinola OB, Caxton-Martins EA (2015) Datura metel Administration distorts medial prefrontal cortex histology of Wistar rats world. J Neurosci 05:282–291Google Scholar
- Fatoba TA, Adeloye AA, Soladoye AO (2013) Effect of Datura stramonium seed extracts on haematological parameters of West African dwarf (WAD) bucks European. J Exp Biol 3:1–6Google Scholar
- Shoubridge E, Jeffry F, Keogh J, Radda G, Seymour A-M (1985) Creatine kinase kinetics, ATP turnover, and cardiac performance in hearts depleted of creatine with the substrate analogue β-guanidinopropionic acid Biochimica et Biophysica Acta (BBA)-Molecular. Cell Res 847:25–32Google Scholar
- Tijani AA, Adeniyi DT, Adekomi DA (2012) Datura metel is deleterious to the visual cortex of adult wistar rats. Adv Appl Sci Res 3:944–949Google Scholar
- Wannang N, Ndukwe H, Nnabuife C (2009) Evaluation of the analgesic properties of the Datura metel seeds aqueous extract. J Med Plant Res 3:192–195Google Scholar
- Weber S, Bloom B, Brown G (1992) Compiling Joy into silicon. In: Advanced Research in VLSI and Parallel Systems, MIT PressGoogle Scholar
- Yadav M, Parle M, Kadian M, Sharma K (2015) A review on psychosis and anti-psychotic plants. Asian J Pharm Clin Res 8:24–28Google Scholar