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Up-regulation of Vps4A promotes neuronal apoptosis after intracerebral hemorrhage in adult rats

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Abstract

Vps4, vacuolar protein sorting 4, belongs to ATPases Associated with diverse cellular Activities (AAA) protein family which is made up of Vps4A and Vps4B. Previous studies demonstrated that Vps4A plays vital roles in diverse aspects such as virus budding, the efficient transport of H-Ras to the PM (plasma membrane) and the involvement in the MVB (multivesiculate bodies) pathway. Interestingly, Vps4A is also expressed in the brain. However, the distribution and function of Vps4A in ICH diseases remain unclear. In this study, we show that Vps4A may be involved in neuronal apoptosis during pathophysiological processes of intracerebral hemorrhage (ICH). Based on the results of Western blot and immunohistochemistry, we found a remarkable up-regulation of Vps4A expression surrounding the hematoma after ICH. Double labeled immunofluorescence showed that Vps4A was co-expressed with NeuN but rarely with astrocytes and microglia. Morever, we detected that neuronal apoptosis marker active caspase-3 had co-localizations with Vps4A. Additionaly, Vps4A knockdown in vitro specifically leads to decreasing neuronal apoptosis coupled with increased Akt phosphorylation. All datas suggested that Vps4A was involved in promoting neuronal apoptosis via inhibiting Akt phosphorylation after ICH.

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Authors and Affiliations

  1. Department of Rehabilitation, the Second People’s Hospital of Nantong, Nantong, Jiangsu Province, 226001, China

    Jianbing Ren, Xuelei Tao, Chenwei Duan, Jianbin Ge & Hongjian Lu

  2. Jiangsu Province Key Laboratory for Inflammation and Molecular Drug Target, Nantong University, Nantong, Jiangsu Province, 226001, China

    Debin Yuan, Lili Xie, Chenwei Duan, Yifeng Bao & Yunfeng He

  3. Department of Neurosurgery, Affiliated Hospital of Nantong University, Nantong, Jiangsu Province, 226001, China

    Debin Yuan, Yifeng Bao & Yunfeng He

  4. Department of Neurology, Affiliated Hospital of Nantong University, Nantong, Jiangsu Province, 226001, China

    Lili Xie

Authors
  1. Jianbing Ren
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  2. Debin Yuan
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  3. Lili Xie
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  4. Xuelei Tao
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  5. Chenwei Duan
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  6. Yifeng Bao
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  7. Yunfeng He
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  8. Jianbin Ge
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  9. Hongjian Lu
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Correspondence to Jianbin Ge or Hongjian Lu.

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No potential conflicts of interest were disclosed.

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Jianbing Ren and Debin Yuan contributed equally to this work.

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Ren, J., Yuan, D., Xie, L. et al. Up-regulation of Vps4A promotes neuronal apoptosis after intracerebral hemorrhage in adult rats. Metab Brain Dis 32, 565–575 (2017). https://doi.org/10.1007/s11011-016-9943-6

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  • DOI: https://doi.org/10.1007/s11011-016-9943-6

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