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Evidence of oxidative stress in brain and liver of young rats submitted to experimental galactosemia

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Abstract

Galactosemia is a disorder of galactose metabolism, leading to the accumulation of this carbohydrate. Galactosemic patients present brain and liver damage. For evaluated oxidative stress, 30-day-old males Wistar rats were divided into two groups: galactose group, that received a single injection of this carbohydrate (5 μmol/g), and control group, that received saline 0.9 % in the same conditions. One, twelve or twenty-four hours after the administration, animals were euthanized and cerebral cortex, cerebellum, and liver were isolated. After one hour, it was found a significant increase in TBA-RS levels, nitrate and nitrite and protein carbonyl contents in cerebral cortex, as well as protein carbonyl content in the cerebellum and in hepatic level of TBA-RS, and a significant decrease in nitrate and nitrite contents in cerebellum. TBA-RS levels were also found increased in all studied tissues, as well as nitrate and nitrite contents in cerebral cortex and cerebellum, that also present increased protein carbonyl content and impairments in the activity of antioxidant enzymes of rats euthanized at twelve hours. Finally, animals euthanized after twenty-four hours present an increase of TBA-RS levels in studied tissues, as well as the protein carbonyl content in cerebellum and liver. These animals also present an increased nitrate and nitrite content and impairment of antioxidant enzymes activities. Taken together, our data suggest that acute galactose administration impairs redox homeostasis in brain and liver of rats.

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Acknowledgments

The present work was supported by grants from University of Southern Santa Catarina, Coordination for the Improvement of Higher Education Personnel, Center for Excellence in Neuroscience of Santa Catarina (Foundation for Support of Scientific and Technological Research of the State of Santa Catarina/Support Program for Centers of Excellence) and National Counsel of Technological and Scientific Development.

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Authors and Affiliations

  1. Laboratório de Erros Inatos do Metabolismo, Programa de Pós-graduação em Ciências da Saúde, Unidade Acadêmica de Ciências da Saúde, Universidade do Extremo Sul Catarinense, Av. Universitária, 1105, bloco S, sala 6, Criciúma, SC, 88806-000, Brazil

    Márcia B. Castro, Bruna K. Ferreira, José Henrique Cararo, Adália E. Chipindo, Marina L. Magenis & Patrícia F. Schuck

  2. Universidade Regional Integrada do Alto Uruguai e das Missões, Erechim, RS, Brazil

    Márcia B. Castro

  3. Laboratório de Fisiopatologia Experimental, Programa de Pós-graduação em Ciências da Saúde, Unidade Acadêmica de Ciências da Saúde, Universidade do Extremo Sul Catarinense, Criciúma, SC, Brazil

    Monique Michels

  4. Laboratório de Imunopatologia Clínica e Experimental, Programa de Pós-graduação em Ciências da Saúde, Universidade do Sul de Santa Catarina, Tubarão, SC, Brazil

    Lucinéia G. Danielski & Fabricia Petronilho

  5. Departamento de Química, Instituto de Ciências Exatas e da Terra, Universidade Federal de Mato Grosso, Cuiabá, MT, Brazil

    Marcos R. de Oliveira

  6. Instituto de Bioquímica Médica Leopoldo de Meis, Universidade Federal do Rio de Janeiro, Rio de Janeiro, RJ, Brazil

    Gustavo C. Ferreira

  7. Laboratório de Bioenergética, Programa de Pós-graduação em Ciências da Saúde, Unidade Acadêmica de Ciências da Saúde, Universidade do Extremo Sul Catarinense, Criciúma, SC, Brazil

    Emilio L. Streck

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  1. Márcia B. Castro
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  2. Bruna K. Ferreira
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Correspondence to Patrícia F. Schuck.

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Castro, M.B., Ferreira, B.K., Cararo, J.H. et al. Evidence of oxidative stress in brain and liver of young rats submitted to experimental galactosemia. Metab Brain Dis 31, 1381–1390 (2016). https://doi.org/10.1007/s11011-016-9865-3

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