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Metabolic Brain Disease

, Volume 31, Issue 5, pp 1105–1112 | Cite as

Reduced glutamate in white matter of male neonates exposed to alcohol in utero: a 1H-magnetic resonance spectroscopy study

  • F. M. HowellsEmail author
  • K. A. Donald
  • A. Roos
  • R. P. Woods
  • H. J. Zar
  • K. L. Narr
  • D. J. Stein
Original Article

Abstract

In utero exposure to alcohol leads to a spectrum of fetal alcohol related disorders (FASD). However, few studies used have used proton magnetic resonance spectroscopy (1H-MRS) to understand how neurochemical disturbances relate to the pathophysiology of FASD. Further, no studies to date have assessed brain metabolites in infants exposed to alcohol in utero. We hypothesize that neonates exposed to alcohol in utero will show decreased glutamatergic activity, pre-emptive of their clinical diagnosis or behavioural phenotype. Single voxel 1H-MRS data, sampled in parietal white and gray matter, were acquired from 36 neonates exposed to alcohol in utero, and 31 control unexposed healthy neonates, in their 2nd-4th week of life. Metabolites relative to creatine with phosophocreatine and metabolites absolute concentrations using a water reference are reported. Male infants exposed to alcohol in utero were found to have reduced concentration of glutamate with glutamine (Glx) in their parietal white matter (PWM), compared to healthy male infants (p = 0.02). Further, male infants exposed to alcohol in utero had reduced concentration and ratio for glutamate (Glu) in their PWM (p = 0.02), compared to healthy male infants and female infants exposed to alcohol in utero. Female infants showed higher relative Glx and Glu ratios for parietal gray matter (PGM, p < 0.01), compared to male infants. We speculate that the decreased Glx and Glu concentrations in PWM are a result of delayed oligodendrocyte maturation, which may be a result of dysfunctional thyroid hormone activity in males exposed to alcohol in utero. Further study is required to elucidate the relationship between Glx and Glu, thyroid hormone activity, and oligodendrocyte maturation in infants exposure to alcohol in utero.

Keywords

MRS Alcohol exposure Gray matter Oligodendrocytes Thyroid hormone 

Abbreviations

1H-MRS

Proton magnetic resonance spectroscopy

FASD

fetal alcohol spectrum disorders

Glx

glutamate with glutamine concentration

Glu

glutamate concentration

PWM

parietal white matter

PGM

parietal gray matter

Notes

Acknowledgments

We thank two MRI experts who assisted during the development of the MRI protocol – Dr André van der Kouwe, MGH Harvard University, USA, and Dr Stefan Blüml, University of Southern California, USA. Mrs Robyn Kalan, our study’s neonatal nurse, who cared for the infants during scan preparation and during the MRI scan. We thank the study staff and the staff at Paarl Hospital, Mbekweni and TC Newman clinics for their support of the study. We also wish to thank the Drakenstein child lung and health research team and staff, led by PI Dr Heather Zar, who without, this study would not have been possible. Lastly, we thank the families and infants who participated in this study.

Compliance with ethical standards

Financial support

The study was supported by a Foundation of Alcohol Research grant as well as the Bill and Melinda Gates Foundation [OPP 1017641]. FMH also acknowledges her funding source, administered by the Institute of Infectious Diseases and Molecular Medicine, University of Cape Town, a Hasso Plattner mid-career development programme award.

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Copyright information

© Springer Science+Business Media New York 2016

Authors and Affiliations

  • F. M. Howells
    • 1
    Email author
  • K. A. Donald
    • 2
  • A. Roos
    • 3
  • R. P. Woods
    • 4
  • H. J. Zar
    • 2
  • K. L. Narr
    • 4
  • D. J. Stein
    • 1
  1. 1.Department of Psychiatry and Mental HealthUniversity of Cape TownCape TownSouth Africa
  2. 2.Department of Paediatrics and Child HealthRed Cross War Memorial Children’s Hospital and University of Cape TownCape TownSouth Africa
  3. 3.Medical Research Council Anxiety and Stress Disorders UnitUniversity of StellenboschStellenboschSouth Africa
  4. 4.Departments of Neurology and of Psychiatry and Biobehavioral SciencesUniversity of California Los AngelesLos AngelesUSA

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