Abstract
Considering that Na+,K+-ATPase is an embedded-membrane enzyme and that experimental chronic hyperprolinemia decreases the activity of this enzyme in brain synaptic plasma membranes, the present study investigated the effect of chronic proline administration on thiobarbituric acid-reactive substances, as well as the influence of antioxidant vitamins E plus C on the effects mediated by proline on Na+,K+-ATPase activity in cerebral cortex of rats. The expression of Na+,K+-ATPase catalytic subunits was also evaluated. Results showed that proline increased thiobarbituric acid-reactive substances, suggesting an increase of lipid peroxidation. Furthermore, concomitant administration of vitamins E plus C significantly prevented the increase of lipid peroxidation, as well as the inhibition of Na+,K+-ATPase activity caused by proline. We did not observe any change in levels of Na+,K+-ATPase mRNA transcripts after chronic exposure to proline and vitamins E plus C. These findings provide insights into the mechanisms through which proline exerts its effects on brain function and suggest that treatment with antioxidants may be beneficial to treat neurological dysfunctions present in hyperprolinemic patients.
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Acknowledgments
This work was supported in part by grants from Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq - Brazil) and Fundação de Amparo à Pesquisa do Estado do Rio Grande do Sul (FAPERGS).
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The authors declare that they have no conflict of interest.
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Ferreira, A.G.K., Stefanello, F.M., Cunha, A.A. et al. Role of antioxidants on Na+,K+-ATPase activity and gene expression in cerebral cortex of hyperprolinemic rats. Metab Brain Dis 26, 141–147 (2011). https://doi.org/10.1007/s11011-011-9243-0
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DOI: https://doi.org/10.1007/s11011-011-9243-0