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Vitamin D accelerates the subdural hematoma clearance through improving the meningeal lymphatic vessel function

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Abstract

Subdural hematoma (SDH) drains into the extracranial lymphatic system through the meningeal lymphatic vessels (mLVs) but the formation of SDH impairs mLVs. Because vitamin D (Vit D) can protect the endothelial cells, we hypothesized that Vit D may enhance the SDH clearance. SDH was induced in Sprague–Dawley rats and treated with Vit D or vehicle. Hematoma volume in each group was measured by H&E staining and hemoglobin quantification. Evans blue (EB) quantification and red blood cells injection were used to evaluated the drainage of mLVs. Western blot analysis and immunofluorescence were conducted to assess the expression of lymphatic protein markers. We also examined the inflammatory factors levels in subdural space by ELISA. Vit D treatment significantly reduced SDH volume and improved the drainage of SDH to cervical lymph nodes. The structure of mLVs in SDH rats were protected by Vit D, and the expressions of LYVE1, PROX1, FOXC2, and VE-cadherin were increased after Vit D treatment. The TNF-α, IL-6, and IL-8 levels were reduced in Vit D group. In vitro, Vit D also increased the VE-cadherin expression levels under inflammation. Vit D protects the structure of mLVs and enhances the absorption of SDH, partly by the anti-inflammatory effect of Vit D.

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The data are available from the corresponding author on reasonable request.

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Funding

This study was funded by the National Natural Science Foundation of China (No. 82271394, No.82071390, No. 81671221).

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  1. Yupeng Chen, Xuanhui Liu and Jiangyuan Yuan have contributed equally to this work.

Authors and Affiliations

  1. Department of Neurosurgery, Tianjin Medical University General Hospital, Tianjin, China

    Yupeng Chen, Xuanhui Liu, Jiangyuan Yuan, Shiying Dong, Meng Nie, Weiwei Jiang, Di Wu, Mingqi Liu, Tao Liu, Chenrui Wu, Chuang Gao, Jianning Zhang & Rongcai Jiang

  2. Ministry of Education, Tianjin Neurological Institute, Key Laboratory of Post Neuro-injury Neuro-repair and Regeneration in Central Nervous System, Tianjin Medical University General Hospital, 154 Anshan Road, Helping District, Tianjin, 300052, China

    Yupeng Chen, Xuanhui Liu, Jiangyuan Yuan, Shiying Dong, Meng Nie, Weiwei Jiang, Di Wu, Mingqi Liu, Tao Liu, Chenrui Wu, Chuang Gao, Jianning Zhang & Rongcai Jiang

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Yupeng Chen, Xuanhui Liu and Jiangyuan Yuan contributed equally to this work. All authors reviewed the manuscript.

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Correspondence to Chuang Gao, Jianning Zhang or Rongcai Jiang.

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Chen, Y., Liu, X., Yuan, J. et al. Vitamin D accelerates the subdural hematoma clearance through improving the meningeal lymphatic vessel function. Mol Cell Biochem (2024). https://doi.org/10.1007/s11010-023-04918-6

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