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Impact of spatial metabolomics on immune-microenvironment in oral cancer prognosis: a clinical report

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Abstract

MALDI imaging for metabolites and immunohistochemistry for 38 immune markers was used to characterize the spatial biology of 2 primary oral tumours, one from a patient with an early recurrence (Tumour R), and the other from a patient with no recurrence 2 years after treatment completion (Tumour NR). Tumour R had an increased purine nucleotide metabolism in different regions of tumour and adenosine-mediated suppression of immune cells compared to Tumour NR. The differentially expressed markers in the different spatial locations in tumour R were CD33, CD163, TGF-β, COX2, PD-L1, CD8 and CD20. These results suggest that altered tumour metabolomics concomitant with a modified immune microenvironment could be a potential marker of recurrence.

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All data has been provided in the manuscript. However if additional information is required will be shared on reasonable request to the corresponding author.

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Acknowledgements

We are grateful to M/S Bruker, Germany, for running the experiments of MALDI.

Funding

GM acknowledges the Department of Biotechnology, Government of India, New Delhi, for funding under the Systems Medicine Cluster (SyMeC, Project Reference: No./BT/Med-II/NIBMG/SyMeC/2014/Vol II).

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Author notes

  1. Swarnendu Bag

    Present address: CSIR-Institute of Genomics and Integrative Biology (IGIB), Mall Road, New Delhi, 110 007, India

Authors and Affiliations

  1. Tata Medical Center, Newtown, Kolkata, 700 160, India

    Swarnendu Bag, Soni Shaikh, Amrita Chaudhary, Pattatheyil Arun & Geetashree Mukherjee

  2. Bruker Daltonics GmbH & Co. KG, Bremen, Germany

    Janina Oetjen

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Contributions

Research planning: GM, SB; Conduct and reporting: GM, JO, SB; Conception and design: GM; Acquisition and interpretation of data: JO, SB, AC, SS; Clinical study: GM, PA; Manuscript preparation: GM, SB, SS.

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Correspondence to Geetashree Mukherjee.

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Competing interest

JO is employed at Bruker Daltonics GmbH Co. KG, a vendor for mass spectrometers. All other authors declare no competing interests.

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Bag, S., Oetjen, J., Shaikh, S. et al. Impact of spatial metabolomics on immune-microenvironment in oral cancer prognosis: a clinical report. Mol Cell Biochem 479, 41–49 (2024). https://doi.org/10.1007/s11010-023-04713-3

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  • DOI: https://doi.org/10.1007/s11010-023-04713-3

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