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The effect of maternal period nutritional status on oro-sensorial fat perception and taste preference in rats

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Abstract

CD36 and GPR120 play an important role in the perception and preference for fat-rich food consumption. We aimed to investigate the relationship between oro-gustatory perception of lipids, fatty taste preference, and maternal (Gestation + Lactation)-maturation period nutrition status in offspring Sprague–Dawley rats. In our study, mother rats were fed with control (C) or high-fat diets (HFD) during gestation (21 days) and lactation (21 days) periods. After weaning, the offspring were fed with control (C) or high-fat diets (HFD) during the maturation (120 days) period. Daily calorie intake and weekly body weight measurements were monitored. Two-bottle preference (TBPT) and licking tests measured the fat perceptions and preferences. Plasma levels of insulin, leptin, glucose, and triglyceride were measured. The protein and mRNA expressions of CD36 and GPR120 in the circumvallate papillae (CVP) were determined. The 48 h TBPT results revealed that maternal HFD-exposed offspring rats significantly preferred 2% rapeseed oil solution regardless of the type of maturation diet. According to the licking test, C/C group (C diet exposed group in maternal and maturation periods) offspring licked 0.1% oleic acid-containing water more than C/HFD (C diet exposed in maternal period and HFD exposed group in maturation period) and HFD/HFD group. (HFD exposed group in maternal and maturation periods) groups. Plasma insulin and leptin concentrations significantly increased in HFD/HFD groups compared to C/C group. CD36 protein expressions were significantly lower in HFD/HFD than C/HFD and HFD/C groups. GPR120 and GNAT3 mRNA expressions in HFD/C group were significantly higher than in C/HFD group. Our results suggest that HFD exposure during maternal and maturation period may play a role in fat perception/preference through oral lipid sensors.

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The datasets generated during and/or analyzed during the current study are available from the corresponding author on reasonable request.

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Acknowledgements

We thank Dr. Deniz Atasoy (Iowa University, Department of Neuroscience and Pharmacology) for his technical advice in designing licking tests.

Funding

This project is supported by The Scientific and Technological Research Council of Turkey (TUBITAK) Project number: 117S384.

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Authors and Affiliations

  1. Faculty of Medicine, Department of Physiology, Yeditepe University, Ataşehir, 34755, Istanbul, Turkey

    Elif Günalan, Meyli Ezgi Karagöz, Cihan Civan Cıvaş, Volkan Adem Bilgin, Cihan Suleyman Erdogan, Aylin Güven, Bayram Yılmaz & Burcu Gemici

  2. Faculty of Health Science, Department of Nutrition and Dietetics, Istanbul Health and Technology University, Istanbul, Turkey

    Elif Günalan

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Contributions

EG: performed experiments, analyzed the results and assisted in writing the manuscript. MEK, CCC and AG: assisted in performing the experiments. VAB and CSE: assisted in writing the manuscript. BY: supervised the project and the manuscript. BG: designed the project, found the budget from TUBITAK, supervised the experimental procedure and experiments, analyzed the data, and wrote the manuscript.

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Correspondence to Bayram Yılmaz or Burcu Gemici.

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The study protocol was approved by the Yeditepe University Experimental Animal Ethics Committee (protocol # 17.12.2016.575).

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Günalan, E., Karagöz, M.E., Cıvaş, C.C. et al. The effect of maternal period nutritional status on oro-sensorial fat perception and taste preference in rats. Mol Cell Biochem 478, 2861–2873 (2023). https://doi.org/10.1007/s11010-023-04703-5

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