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Effects of four weeks lasting aerobic physical activity on cardiovascular biomarkers, oxidative stress and histomorphometric changes of heart and aorta in rats with experimentally induced hyperhomocysteinemia

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Abstract

The aim of this study was to examine the effects of hyperhomocysteinemia and aerobic physical activity on changes of cardiovascular biomarkers in sera, oxidative stress in cardiac tissue, and histomorphometric parameters of heart and aorta in rats. Experiments were conducted on male Wistar albino rats organized into four groups (n = 10, per group): C (control group): 0.9% NaCl 0.2 mL/day; H (homocysteine group): homocysteine 0.45 µmol/g b.w./day; CPA (control + physical activity group): 0.9% NaCl 0.2 mL/day and a program of physical activity on a treadmill; and HPA (homocysteine + physical activity group) homocysteine 0.45 µmol/g b.w./day and a program of physical activity on a treadmill. Substances were applied subcutaneously twice a day. Lipid peroxidation and relative activity of Mn-superoxide dismutase isoform were significantly higher in active hyperhomocysteinemic rats in comparison to sedentary animals. Atherosclerotic plaques were detected in aorta samples of active hyperhomocysteinemic rats and also, they had increased left ventricle wall and interventricular septum, and transverse diameter of cardiomyocytes compared to sedentary groups. Aerobic physical activity in the condition of hyperhomocysteinemia can lead to increased oxidative stress in cardiac tissue and changes in histomorphometric parameters of the heart and aorta, as well increased lipid parameters and cardiac damage biomarkers in sera of rats.

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The datasets generated during and/or analyzed during the current study are not publicly available, but are available from the corresponding author on reasonable request.

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Funding

This research was supported by the Ministry of Education, Science and Technological Development of the Republic of Serbia (Grant No. 175043 and No. 200110).

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Authors and Affiliations

  1. Institute of Medical Physiology “Richard Burian”, Faculty of Medicine, University of Belgrade, Visegradska 26/II, 11000, Belgrade, Serbia

    Dusan Todorovic, Marija Stojanovic & Dragan Djuric

  2. Institute of Chemistry in Medicine “Prof. Dr. Petar Matavulj”, Faculty of Medicine, University of Belgrade, Belgrade, Serbia

    Kristina Gopcevic & Ana Medic

  3. Centre of Medical Biochemistry, Clinical Centre of Serbia, Belgrade, Serbia

    Sanja Stankovic

  4. Faculty of Medicine, University of Belgrade, Belgrade, Serbia

    Boba Kotlica

  5. Institute of Histology and Embryology “Aleksandar Dj. Kostic”, Faculty of Medicine, University of Belgrade, Belgrade, Serbia

    Milica Labudovic Borovic

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  1. Dusan Todorovic
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  2. Marija Stojanovic
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  4. Ana Medic
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Contributions

DD contributed to the study conception, design, organization and funding. Material preparation, data collection and analysis were performed by DT, MS, AM, SS, and MLB. The first draft of the manuscript was written by DT and BK. All authors commented on previous versions of the manuscript. All authors read and approved the final manuscript.

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Correspondence to Dragan Djuric.

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The authors declare that the research was conducted in the absence of any commercial or financial relationships that could be construed as a potential conflict of interest.

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The study has been approved by the Ethical Council for the Welfare of Experimental Animals, Ministry of Agriculture, Forestry and Water Management, Veterinary Directorate, Republic of Serbia No: 323-07-02523/2018-05.

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Todorovic, D., Stojanovic, M., Gopcevic, K. et al. Effects of four weeks lasting aerobic physical activity on cardiovascular biomarkers, oxidative stress and histomorphometric changes of heart and aorta in rats with experimentally induced hyperhomocysteinemia. Mol Cell Biochem 478, 161–172 (2023). https://doi.org/10.1007/s11010-022-04503-3

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