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Hsa_circ_0006732 regulates colorectal cancer cell proliferation, invasion and EMT by miR-127-5p/RAB3D axis

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Abstract

Colorectal cancer (CRC) remains a malignancy tumor with high metastasis and poor prognosis. We aimed to explore the effect of circular RNA (circRNA) hsa_circ_0006732 in the progression of CRC. Hsa_circ_0006732 expression in CRC tissues and cell lines were detected using qRT-PCR. The relationship between hsa_circ_0006732 expression and clinicopathologic characteristics of patients with CRC was analyzed. Loss-of-function assay was conducted to determine the regulatory effect of hsa_circ_0006732 on CRC cell proliferation, migration and invasion by using the CCK-8, wound-healing assay and transwell assays. Protein expression changes on epithelial mesenchymal transition (EMT)-related factors were detected by western blotting. The downstream signaling pathway was investigated by bioinformatics, dual-luciferase reporter assay. Rescue assay was further examined for prediction validation. It was found that hsa_circ_0006732 was highly expressed in CRC tissues and cell lines. Downregulation of hsa_circ_0006732 suppressed the proliferation, migration, invasion and EMT of CRC cells. Further mechanistic investigations proved that hsa_circ_0006732 functioned as a competitive endogenous RNA (ceRNA) by directly sponging of miR-127-3p, which further affected the expression of Ras-related protein Rab-3D (Rab3D). Taken together, these findings indicated that hsa_circ_0006732 might be an oncogene in CRC through the regulation of the miR-127-5p/RAB3D axis. Thus, hsa_circ_0006732 might serve as a potential therapeutic target for the treatment of CRC.

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Acknowledgements

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The authors declare that they have no funding support.

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Authors and Affiliations

  1. The First Affiliated Hospital of Jinan University, Jinan University, Guangzhou, 510000, China

    Tao Yang & Zhongkai Fan

  2. Department of General Surgery, The First Affiliated Hospital of Jinzhou Medical University, Jinzhou, 121010, China

    Tao Yang, Wei Wang & Dongsheng Li

  3. Orthopedic Department, First Affiliated Hospital of Jinzhou Medical University, No.2 Renmin Street, Jinzhou, 121000, China

    Jufeng Sun, Xianxu Yang, Ang Jia, Yinda Ma & Zhongkai Fan

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  1. Tao Yang
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  2. Jufeng Sun
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  3. Wei Wang
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  4. Dongsheng Li
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Contributions

TY, JFS conceived and designed the study. WW and DSL performed the literature search and data extraction. XXY, AJ, YDM and ZF drafted the manuscript. All authors had read and approved the final manuscript.

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Correspondence to Zhongkai Fan.

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The experimental protocol was established, according to the ethical guidelines of the Helsinki Declaration and was approved by the Animal Ethics Committee of First Affiliated Hospital of Jinzhou Medical University.

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Yang, T., Sun, J., Wang, W. et al. Hsa_circ_0006732 regulates colorectal cancer cell proliferation, invasion and EMT by miR-127-5p/RAB3D axis. Mol Cell Biochem 477, 2751–2760 (2022). https://doi.org/10.1007/s11010-022-04458-5

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  • DOI: https://doi.org/10.1007/s11010-022-04458-5

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