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MicroRNA-1297 participates in the repair of intestinal barrier injury in patients with HIV/AIDS via negative regulation of PLCβ1

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Abstract

To explore the role of the miRNA-1297/phospholipase Cβ1 (PLCβ1) axis in intestinal barrier injury. Abnormally expressed miR-1297 and its target gene PLCβ1 as well as their transcriptome sequencing were confirmed by bioinformatics analysis. Next, the intestinal barrier injury was induced by lipopolysaccharide (LPS) in the CCCHIE-2 cells. Subsequently, the impacts of miR-1297 and PLCβ1 on the transcriptome were estimated. QRT-PCR and Western blotting were conducted to detect the relative mRNA and protein expressions, respectively. The cell viability and permeability were analyzed by MTT assay and fluorescent yellow detection. miR-1297 was significantly upregulated in patients with human immunodeficiency virus/acquired immunodeficiency syndrome and targeted PLCβ1. Moreover, overexpressed PLCβ1 was mainly enriched in the transforming growth factor-beta signaling pathway, while the knockdown of miR-1297 was focused on the arginine biosynthesis pathway. The overexpression of miR-1297 could reduce the PLCβ1 expression and inhibit the viability of CCCHIE-2 cells injured by LPS, while the effect of the downregulation of miR-1297 was on the opposite. Western blotting and cell fluorescence localization experiments revealed that the inhibition of miR-1297 increased the expressions of PLCβ1 and ZO-1. In addition, the upregulation of miR-1297 strengthened the permeability in cells injured by LPS, as did the knockdown of PLCβ1. miR-1297 could restrain the repair of intestinal barrier injury via negatively regulating PLCβ1 and its tight junction downstream protein ZO-1 in CCC-HIE-2 cells injured by LPS, which indicated that PLCβ1 and miR-1297 might be important targets for the repair of intestinal barrier injury.

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Funding

This research was supported by the Basic Research Plan of Yunnan Province (Joint Project of Kunming Medical College) (No. 2019FE001 (-221)).

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Authors and Affiliations

  1. Department of Clinical Laboratory, The First Affiliated Hospital of Kunming Medical University, Kunming, 650032, Yunnan, China

    Yuxia Bao

  2. Yunnan Institute of Experimental Diagnosis, Kunming, 650032, Yunnan, China

    Yuxia Bao

  3. Yunnan Key Laboratory of Laboratory Medicine, Kunming, 650032, Yunnan, China

    Yuxia Bao

  4. Yunnan Engineering Technology Center of Diagnosis and Treatment of Digestive Diseases, Kunming, 650032, Yunnan, China

    Huiming Guo, Fengrong Chen & Zunyue Zhang

  5. Department of Vascular Surgery, The First Affiliated Hospital of Kunming Medical University, No. 295 Xichang Road, Kunming, 650032, Yunnan, China

    Bin Yang & Jianyuan Gao

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  1. Yuxia Bao
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  2. Huiming Guo
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  3. Bin Yang
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  4. Fengrong Chen
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Contributions

JG conceived and supervised the study; YB and JG designed the experiments; YB and HG performed the experiments; BY provided new tools and reagents; FC and ZZ collected and analyzed the data; YB wrote the manuscript; JG made manuscript revisions.

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Correspondence to Jianyuan Gao.

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This study was approved by the Ethics Committee of the First Affiliated Hospital of Kunming Medical University and was in accordance with the National Institutes of Health guide for the care and use of laboratory animals for the care and use of laboratory animals.

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Bao, Y., Guo, H., Yang, B. et al. MicroRNA-1297 participates in the repair of intestinal barrier injury in patients with HIV/AIDS via negative regulation of PLCβ1. Mol Cell Biochem 477, 2133–2147 (2022). https://doi.org/10.1007/s11010-022-04426-z

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