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Quantitative proteomics analysis of human vitreous in rhegmatogenous retinal detachment associated with choroidal detachment by data-independent acquisition mass spectrometry

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Abstract

The prognosis of rhegmatogenous retinal detachment (RRD) with choroidal detachment (RRDCD) is often poor and complicated. This study focused on the identification of the characteristic proteins and signal pathways associated with the etiology of RRDCD and to provide guidance for diagnosis and treatment of RRDCD. In this study, vitreous humor samples were obtained from 16 RRDCD patients, 14 with RRD, 12 with idiopathic epiretinal macular membrane (IEMM), and 5 healthy controls from donated corpse eyes. Data-independent acquisition mass spectrometry and bioinformatics analysis were employed to identify differentially expressed proteins (DEPs). In the vitreous humor, 14,842 peptides were identified. Patients with RRDCD had 249 DEPs (93 upregulated and 156 downregulated), with 89 in patients with RRD and 61 in patients with IEMM. Enrichment analysis of the GO and Kyoto Encyclopedia of Genes and Genomes DEP databases indicated functional clusters related to inflammation and immunity, protein degradation and absorption, cell adhesion molecules (CAMs), the hedgehog signaling pathway, and lipid metabolism. Weighted gene co-expression network analysis showed that DEPs with positive co-expression of RRDCD participated in immune-related pathways led by the complement and coagulation cascade, whereas DEPs with negative co-expression of RRDCD participated in protein degradation and absorption, CAMs, and the hedgehog signaling pathway. In summary, our study provides important clues and the theoretical basis for exploring the pathogenesis, progression, and prognosis of ocular fundus disease.

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Data availability

The datasets analyzed during the current study are available from the corresponding author on reasonable request.

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Acknowledgements

We thank the Shanghai Applied Protein Technology Co., Ltd. (APTBIO) for technological assistance.

Funding

This study was supported by the Social Development Project of Jiangsu Provincial Science and Technology Department (BE2017627), the high-end talent project of Wuxi Health Committee, the youth project of Wuxi Health Committee (Q202038), and the reserve top talent project of Wuxi Municipal Health Commission (HB2020032).

Author information

Author notes

  1. Shasha Luo and Huiyan Xu have contributed equally.

Authors and Affiliations

  1. Department of Ophthalmology, Nanjing Medical University Affiliated Wuxi Second Hospital, 68 Zhongshan Road, Wuxi, 214002, Jiangsu Province, People’s Republic of China

    Shasha Luo, Huiyan Xu, Lufei Yang, Xuechun Gong, Jinyan Shen, Xuan Chen & Zhifeng Wu

  2. Department of Ophthalmology, Affiliated Wuxi Clinical College of Nantong University, Wuxi, Jiangsu Province, People’s Republic of China

    Shasha Luo, Huiyan Xu & Zhifeng Wu

Authors
  1. Shasha Luo
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  2. Huiyan Xu
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  3. Lufei Yang
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  4. Xuechun Gong
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  5. Jinyan Shen
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  6. Xuan Chen
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  7. Zhifeng Wu
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Contributions

Conceptualization, ZW and SL; methodology, SL and HX; formal analysis, SL; investigation, SL, HX, LY, and XG; resources, JS and XC; writing—original draft preparation, SL and HX; writing—review and editing, ZW.

Corresponding author

Correspondence to Zhifeng Wu.

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Conflict of interest

The authors declare that the research was conducted in the absence of any commercial or financial relationships that could be construed as a potential conflict of interest.

Ethical approval

This study adhered to the guidelines of the Declaration of Helsinki and the ethics committee of Nanjing Medical University affiliated Wuxi No. 2 Hospital approved the study (Wuxi, China. Identification code: 2019Y-30).

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All the participating patients in this study provided signed, informed consent prior to the study.

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Luo, S., Xu, H., Yang, L. et al. Quantitative proteomics analysis of human vitreous in rhegmatogenous retinal detachment associated with choroidal detachment by data-independent acquisition mass spectrometry. Mol Cell Biochem 477, 1849–1863 (2022). https://doi.org/10.1007/s11010-022-04409-0

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  • DOI: https://doi.org/10.1007/s11010-022-04409-0

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