Abstract
This study investigated the regulatory effects of microRNA-1278 (miR-1278) on airway inflammation, airway reconstruction, and the proliferation and apoptosis of airway smooth muscle cells (ASMCs) induced by transforming growth factor β1 (TGF-β1). The results showed that miR-1278 was upregulated in the blood and lung tissues (LTs) of patients with asthma compared with that in healthy volunteers; miR-1278 expression was also upregulated in asthmatic mice, and miR-1278 inhibition improved the LTs of asthmatic mice. Moreover, miR-1278 inhibited inflammation in asthmatic mice and counteracted the effect of TGF-β1 of induced proliferation and reduced apoptosis in ASMCs. DLRA indicated that miR-1278 targeted the 3′-UTR of Src-homology 2-containing phosphatase 1 (SHP-1). Furthermore, miR-1278 promoted ASMC proliferation, in which TGF-β1 played an important role by regulating the SHP-1/STAT3 signaling pathway. In conclusion, this study showed that miR-1278 played a critical role in the processes of airway remodeling and reduction of apoptosis by targeting SHP-1.
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The data used to support the findings of this study are available from the corresponding author upon request.
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This work was supported by the NSFC (No. 81770028); the Key Laboratory of Shenzhen Respiratory Disease (No. ZDSYS201504301616234) and the Clinical Research of Shenzhen Municipal Health and Family Planning Commission (No. SZLY2017024). This work also was supported by special funding for high-level disciplines from the Shenzhen Institute of Respiratory Diseases.
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JL, RC, YL and YZ conceived and designed the research and interpreted the results of experiments. JL and RC performed experiments, analyzed data. All authors approved final version of manuscript. JL and RC edited and revised the manuscript.
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All procedures were reviewed and approved by the Ethics Committee (EC) of Shenzhen People’s Hospital (The First Affiliated Hospital of Southern University of Science and Technology, The Second Clinical Medical College of Jinan University).
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Li, J., Chen, R., Lu, Y. et al. The microRNA-1278/SHP-1/STAT3 pathway is involved in airway smooth muscle cell proliferation in a model of severe asthma both intracellularly and extracellularly. Mol Cell Biochem 477, 1439–1451 (2022). https://doi.org/10.1007/s11010-022-04358-8
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DOI: https://doi.org/10.1007/s11010-022-04358-8