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TP53-mediated miR-2861 promotes osteogenic differentiation of BMSCs by targeting Smad7

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Molecular and Cellular Biochemistry Aims and scope Submit manuscript

Abstract

Bone defect seriously affects the quality of life. Meanwhile, osteogenic differentiation in BMSCs could regulate the progression of bone defect. Transcription factors are known to regulate the osteogenic differentiation in BMSCs. The study aimed to investigate the detailed mechanism by which TP53 regulates the osteogenic differentiation. To study bone defect in vitro, BMSCs were isolated from spinal cord injury rats. CCK-8 assay was applied to test the cell viability. The mineralized nodules in BMSCs was tested by alizarin red staining. Meanwhile, TUNEL staining and flow cytometry were performed to test the cell apoptosis. mRNA expression was tested by qRT-PCR. Starbase and dual-luciferase reporter assay were used to predict the downstream mRNA of miR-2861. Moreover, western blot was applied to detect the protein expressions (TP53 and Smad7). BMSCs were successfully isolated from rats. The expressions of miR-2861 were significantly upregulated in osteogenic medium, compared with growth medium. MiR-2861 inhibitor significantly decreased the levels of OCN, ALP, BSP, and Runx2 in BMSCs. In addition, miR-2861 inhibitor notably inhibited the mineralized nodules, viability, and induced the apoptosis of BMSCs. Smad7 was identified to be the downstream target of miR-2861, and knockdown of Smad7 notably reversed miR-2861 inhibitor-induced inhibition of osteogenic differentiation and promotion of apoptosis in BMSCs. Moreover, miR-2861 was transcriptionally regulated by TP53 in BMSCs. TP53-meidiated miR-2861 promotes osteogenic differentiation of BMSCs by targeting Smad7. Thereby, our research might provide new methods for bone defect treatment.

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Data availability

The datasets used or analyzed during the current study are available from the corresponding author on reasonable request.

Code availability

Not Applicable.

Abbreviations

BMSCs:

Bone marrow mesenchymal stem cells

TP53:

Tumor protein P53

miRNAs:

MicroRNAs

DMEM:

Dulbecco’s modified eagle medium

GM:

Growth medium

OM:

Osteogenic medium

FBS:

Fetal bovine serum

CCK-8:

Cell counting kit-8

qRT-PCR:

Quantitative real-time PCR

ChIP:

Chromatin immunoprecipitation

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Authors and Affiliations

  1. Department of Hand and Foot Surgery, Brain Hospital of Hunan Province, No. 427, Section 3 of Furong Middle Road, Changsha, 410007, Hunan Province, China

    Xian-Pei Zhou, Qi-Wei Li & Zi-Zhen Shu

  2. Department of Gastrointestinal Surgery, Second Xiangaya Hospital, Central South University, No. 139 Renmin Road, Furong District, Changsha, 410011, Hunan Province, China

    Yang Liu

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  1. Xian-Pei Zhou
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  4. Yang Liu
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Contributions

X-PZ made substantial contributions to the conception or design of the work and revised it critically for important intellectual content; Q-WL agrees to be accountable for all aspects of the work in ensuring that questions related to the accuracy or integrity of any part of the work are appropriately investigated and resolved; Z-ZS drafted the work; YL performed the acquisition, analysis, and interpretation of data; All authors approved the version to be published.

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Correspondence to Xian-Pei Zhou or Yang Liu.

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Zhou, XP., Li, QW., Shu, ZZ. et al. TP53-mediated miR-2861 promotes osteogenic differentiation of BMSCs by targeting Smad7. Mol Cell Biochem 477, 283–293 (2022). https://doi.org/10.1007/s11010-021-04276-1

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  • DOI: https://doi.org/10.1007/s11010-021-04276-1

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