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A study of hydrophobins-modified menaquinone-7 on osteoblastic cells differentiation

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A Correction to this article was published on 11 July 2023

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Abstract

Menaquinone-7 is involved in bone metabolism and can be used to prevent and treat osteoporosis. However, as a fat-soluble vitamin, menaquinone-7 has poor water solubility. As a surfactant, hydrophobins can change the affinity/hydrophobicity of the covered interface. In this study, menaquinone-7 was modified by hydrophobins, and the different addition ratios were explored. Moreover, Fourier transform infrared (FTIR), X-ray photoelectron spectroscopy (XPS), and water contact angle (WCA) measurements indicated that hydrophobins effectively bind to menaquinone-7 and greatly increase the hydrophilicity of the surface of menaquinone-7. Studies on the metabolism of MC3T3-E1 cells showed that compared with native menaquinone-7, HGFI-modified menaquinone-7 can significantly promote osteoblast differentiation but inhibit osteoclast differentiation. Besides, the Mito-Tracker Green experiments show that HGFI-modified menaquinone-7 can significantly promote the activity of mitochondria in cells. These findings indicate that hydrophobins can be used as an effective biomaterial to modify menaquinone-7, promote the formation of osteoblasts, and better to bone balance.

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Acknowledgements

This work was supported by the National Key R&D Program of China [Grant Number 2019YFA0904300 (2019YFA0904304)], the Key research and development plan of Anhui Province (Grant Number 1804b06020342), the Natural Science Foundation of Anhui Province (Grant Nos. 1908085MB48, 1908085MB43), and the Anhui Province key research and development program (Grant Number 202004b11020014).

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Authors and Affiliations

  1. Key Laboratory of High Magnetic Field and Ion Beam Physical Biology, Hefei Institutes of Physical Science, Chinese Academy of Sciences, Hefei, 230031, People’s Republic of China

    Hengfang Tang, Zhu Zhu, Zhiming Zheng, Han Wang, Chu Li, Li Wang, Genhai Zhao & Peng Wang

  2. Science Island Branch of Graduate, University of Science and Technology of China, Hefei, 230026, People’s Republic of China

    Hengfang Tang & Zhu Zhu

  3. Hefei Institute of Technology Innovation Engineering, CAS, Hefei, People’s Republic of China

    Zhiming Zheng & Peng Wang

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  1. Hengfang Tang
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Correspondence to Zhiming Zheng or Peng Wang.

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Tang, H., Zhu, Z., Zheng, Z. et al. A study of hydrophobins-modified menaquinone-7 on osteoblastic cells differentiation. Mol Cell Biochem 476, 1939–1948 (2021). https://doi.org/10.1007/s11010-021-04062-z

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